Study of mechanism and control of bacterial translocation from the gastrointestinal tract

• Project/Area Number: 24591491
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: Tokyo Medical University
• Principal Investigator: MATSUMOTO Tetsuya 東京医科大学, 医学部, 教授 (10256688)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: バクテリアル・トランスロケーション / 腸内細菌 / プロバイオティクス / 緑膿菌 / サルモネラ / 菌血症・敗血症 / 炎症性腸疾患

Outline of Final Research Achievements

Bacterial translocation (BT) is defined as the passage of viable bacteria from the gastrointestinal tract to extraintestinal sites via lymphatic and/or blood vessel routes. We studied the mechanisms of BT by using immunosuppressive mouse model with cyclophosphamide treatment and the results showed intragastric administration of Pseudomonas aeruginosa frequently cause systemic spread of translocating bacteria to cause lethal sepsis. Administration of ampicillin induced exacerbation of BT and sepsis.

Research Products

• [Journal Article] Effectiveness of antibiotic combination therapy as evaluated by the Break-point Checkerboard Plate method for multidrug-resistant Pseudomonas aeruginosa in clinical use. (2014)
  • Author(s): Nakamura I, Yamaguchi T, Tsukimori A, Sato A, Fukushima S, Mizuno Y, Matsumoto T.
  • Journal Title: J Infect Chemother. Volume: 20 Issue: 4 Pages: 266-269
  • DOI: 10.1016/j.jiac.2013.12.005
  • Peer Reviewed
• [Journal Article] In vivo challenging of polymyxins and levofloxacin eye drop against multidrug-resistant Pseudomonas aeruginosa keratitis. (2014)
  • Author(s): Tajima K, Miyake T, Koike N, Hattori T, Kumakura S, Yamaguchi T, Matsumoto T, Fujita K, Kuroda M, Ito N, Goto H.
  • Journal Title: J Infect Chemother. Volume: 20 Issue: 6 Pages: 343-349
  • DOI: 10.1016/j.jiac.2013.10.015
  • Peer Reviewed
• [Journal Article] Arbekacin: another novel agent for treating infections due to methicillin-resistant Staphylococcus aureus and multidrug-resistant Gram-negative pathogens. (2014)
  • Author(s): Matsumoto T.
  • Journal Title: Clinical Pharmacology: Advances and Applications Volume: 6 Pages: 139-148
  • DOI: 10.2147/cpaa.s44377
  • Peer Reviewed
• [Journal Article] Clinical aspects of infection with methicillin-resistant Staphylococcus aureus USA300 strain, generally regarded as community-acquired, in Japan. (2013)
  • Author(s): Nakamura I, Yamaguchi T, Miura Y, Shimizu H, Fukushima S, Mizuno Y, Matsumoto T.
  • Journal Title: Jpn J Infect Dis. Volume: 5 Pages: 416-420
  • NAID: 130003381719
  • Peer Reviewed
• [Journal Article] Antimicrobial susceptibility of Enterococcus strains used in clinical practice as probiotics. (2013)
  • Author(s): Yamaguchi T, Miura Y, Matsumoto T.
  • Journal Title: J Infect Chemother. Volume: 6 Issue: 6 Pages: 1109-1115
  • DOI: 10.1007/s10156-013-0633-6
  • Peer Reviewed
• [Journal Article] Activation of COL1A2 promoter in human fibroblasts by Escherichia coli. (2012)
  • Author(s): Miyazaki H, Kobayashi R, Ishikawa H, Awano N, Yamagoe S, Miyazaki Y, Matsumoto T.
  • Journal Title: FEMS Immunol Med Microbiol Volume: 65 Issue: 3 Pages: 481-487
  • DOI: 10.1111/j.1574-695x.2012.00979.x
  • Peer Reviewed
• [Journal Article] Inhibitory effects of lactoferrin on biofilm formation in clinical isolates of Pseudomonas aeruginosa (2012)
  • Author(s): Kamiya H, Ehara T, Matsumoto T
  • Journal Title: J Infect Chemother Volume: 18 Issue: 1 Pages: 47-52
  • DOI: 10.1007/s10156-011-0287-1
  • NAID: 10030226366
  • Peer Reviewed
• [Presentation] fficacy Of Combination Therapy Against Multidrug-resistant Pseudomonas aeruginosa Compared With Monotherapy Against Drug-sensitive Pseudomonas aeruginosa (2014)
  • Author(s): I. Nakamura, H. Fujita, A. Tsukimori, T. Kobayashi, A. Sato, S. Fukushima, Y. Mizuno, T. Matsumoto.
  • Organizer: Interscience Conference of Antimicrobial Agents and Chemotherapy
  • Place of Presentation: Washington DC (USA).
  • Year and Date: 2014-09-07
• [Presentation] Evaluation of a PCR Assay for Multiple Detection of Pathogens causing Community- Acquired Pneumonia. (2013)
  • Author(s): T. Matsumoto, T. Yamaguchi, R. Sekizawa, Y. Kuwata, R. Aso, H. Mitsutake, M. Fujimoto, K. Tsuruta.
  • Organizer: 28th International Congress of Chemotherapy and Infection
  • Place of Presentation: Yokohama
• [Remarks] 東京医科大学微生物学講座
  • URL: http://www.tokyo-med.ac.jp/microbiology/
• [Remarks] 東京医科大学微生物学講座
  • URL: http://www.tokyo-med.ac.jp/microbiology/
• [Remarks] 東京医科大学微生物学講座
  • URL: http://www.tokyo-med.ac.jp/microbiology/