| Project/Area Number |
24591492
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Infectious disease medicine
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| Research Institution | Hokuriku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SADARARI Hidetaka 北陸大学, 教育能力開発センター, 助教 (60121274)
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| Co-Investigator(Renkei-kenkyūsha) |
EIZURU Yoshito 鹿児島大学, 医学部, 名誉教授 (00041351)
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| Research Collaborator |
YAMADA Rie 北陸大学, 薬学部, 助手
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | サイトメガロウイルス / トリシン / 抗ウイルス薬 / ケモカイン / STAT3 / siRNA |
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| Outline of Final Research Achievements |
It has been reported that treatment with tricin (4’,5,7-trihydroxy-3’,5’-dimethoxyflavone), a material in the rice family plants, after human cytomegalovirus (HCMV) infection significantly suppressed infectious virus production in the human embryonic fibroblast cell line (HEL). In this paper, we examined the mechanisms for the anti-HCMV activities of tricin in HEL cells. Exposure of fibroblasts to tricin inhibited infectious HCMV production, with concomitant decreases in levels of transcripts of the CXC chemokine (CXCL11) and CC chemokine (CCL2) genes. We also found that the transcripts of the HCMV immediate early (IE) gene and replication of HCMV were lower in CXCL11 and/or CCL2 gene-knockdown cells. These results suggest that tricin is a novel compound with potential anti-HCMV activity and that CXCL11 and/or CCL2 are one of the chemokines involved in HCMV replication. In addition, it is possible that CXCL11 and/or CCL2 are the one of the targets of tricin.
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