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Identifying the regulatory mechanisms and molecular functions of transcription factor, SF1, during ovarian development in mice

Research Project

Project/Area Number 24591503
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pediatrics
Research InstitutionTokyo Medical and Dental University

Principal Investigator

KASHIMADA Kenichi  東京医科歯科大学, 医学部附属病院, 講師 (80451938)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords性分化 / 卵巣発生 / SF1 / 再生医学 / 転写制御 / Sex determination / ovarian development / transcription factor / FOXL2 / WT1
Outline of Final Research Achievements

Steroidogenic factor 1 (SF1) plays key roles in gonadal development. Initially, the Sf1 gene is expressed in mouse fetal gonads of both sexes from 9.5 dpc, but later is up-regulated in testes and down-regulated in ovaries after sex determination. While Sf1 expression is activated and maintained by Wilms Tumor 1 (WT1) and LIM homeobox 9 (LHX9), the mechanism of sex-specific regulation remains unclear, especially suppressive mechanisms in ovary. We focused on the transcription factor Forkhead box L2 (FOXL2) which has been considered to be important for ovarian folliculogenesis and granulosa cell development. By using in vitro analysis and Foxl2 knock out mice, we showed that FOXL2 negatively regulates Sf1 expression by antagonizing WT1-KTS during early ovarian development in mice and provide new mechanistic insight into the differentiation of ovaries, especially granulosa cell differentiation.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (9 results)

All 2015 2014 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (6 results) Remarks (2 results)

  • [Journal Article] FOXL2 transcriptionally represses Sf1 expression by antagonizing WT1 during ovarian development in mice2014

    • Author(s)
      Takasawa K, Kashimada K, Pelosi E, Takagi M, Morio T, Asahara H, Schlessinger D, Mizutani S, Koopman P
    • Journal Title

      The FASEB Journal

      Volume: 28 (5) Issue: 5 Pages: 2020-28

    • DOI

      10.1096/fj.13-246108

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed
  • [Presentation] FOXL2 transcriptionally represses Sf1 expression by antagonizing WT1 during ovarian development in mice2015

    • Author(s)
      Kei Takasawa, Kenichi Kashimada, Emanuele Pelosi, Masatoshi Takagi, Tomohiro Morio, Hiroshi Asahara, David Schlessinger, Shuki Mizutani, Peter Koopman
    • Organizer
      7th International Symposium on the Biology of Vertebrate Sex Determination
    • Place of Presentation
      USA, Hawaii, Kona
    • Year and Date
      2015-04-13 – 2015-04-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] 卵巣発生初期において卵巣特異的転写因子FOXL2はWT1-KTSに拮抗しSf1の発現を抑制する2015

    • Author(s)
      高澤啓, Emanuele Pelos, 高木正稔, Dagmar Wilhelm, 森尾友宏, 浅原弘嗣, David Schlessinger, 水谷修紀, Peter Koopman, 鹿島田健一
    • Organizer
      日本生殖内分泌学会
    • Place of Presentation
      大阪市 千里ライフサイエンスセンタ―
    • Year and Date
      2015-01-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] 卵巣発生初期において卵巣特異的転写因子FOXL2はWT1-KTSに拮抗しSf1の発現を抑制する2014

    • Author(s)
      高澤啓, Emanuele Pelos, 高木正稔, Dagmar Wilhelm, 森尾友宏, 浅原弘嗣, David Schlessinger, 水谷修紀, Peter Koopman, 鹿島田健一
    • Organizer
      日本小児内分泌学会
    • Place of Presentation
      浜松オークラアクトシティ
    • Year and Date
      2014-09-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] FOXL2 transcriptionally represses Sf1 expression by antagonizing WT1 during ovarian development in mice2014

    • Author(s)
      Kei Takasawa, Kenichi Kashimada, Emanuele Pelosi, Masatoshi Takagi, Tomohiro Morio, Hiroshi Asahara, David Schlessinger, Shuki Mizutani, Peter Koopman
    • Organizer
      米国内分泌学会
    • Place of Presentation
      シカゴ
    • Year and Date
      2014-06-21 – 2014-06-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] 卵巣発生初期において卵巣特異的転写因子FOXL2はWT1-KTSに拮抗しSf1の発現を抑制する2014

    • Author(s)
      高澤啓, Emanuele Pelos, 高木正稔, Dagmar Wilhelm, 森尾友宏, 浅原弘嗣, David Schlessinger, 水谷修紀, Peter Koopman, 鹿島田健一
    • Organizer
      日本内分泌学会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2014-04-24 – 2014-04-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] O-3-9-1 卵巣発生初期において卵巣特異的転写因子 FOXL 2 は WT 1 -KTS に拮抗し Ad 4 BP/SF- 1 の発現 を抑制する2014

    • Author(s)
      高澤啓, 高木正稔, 浅原弘嗣, 森尾友宏, 水谷修紀, D Schlessinger, P. Koopman, 鹿島田健一
    • Organizer
      第87回日本内分泌学会
    • Place of Presentation
      福岡
    • Related Report
      2013 Research-status Report
  • [Remarks] 東京医科歯科大学小児科 ホームページ

    • URL

      http://www.tmd.ac.jp/med/ped/home.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 東京医科歯科大学小児科学教室 新着情報

    • URL

      http://www.tmd.ac.jp/med/ped/index.html

    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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