Identifying the regulatory mechanisms and molecular functions of transcription factor, SF1, during ovarian development in mice
| Project/Area Number |
24591503
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 性分化 / 卵巣発生 / SF1 / 再生医学 / 転写制御 / Sex determination / ovarian development / transcription factor / FOXL2 / WT1 |
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| Outline of Final Research Achievements |
Steroidogenic factor 1 (SF1) plays key roles in gonadal development. Initially, the Sf1 gene is expressed in mouse fetal gonads of both sexes from 9.5 dpc, but later is up-regulated in testes and down-regulated in ovaries after sex determination. While Sf1 expression is activated and maintained by Wilms Tumor 1 (WT1) and LIM homeobox 9 (LHX9), the mechanism of sex-specific regulation remains unclear, especially suppressive mechanisms in ovary. We focused on the transcription factor Forkhead box L2 (FOXL2) which has been considered to be important for ovarian folliculogenesis and granulosa cell development. By using in vitro analysis and Foxl2 knock out mice, we showed that FOXL2 negatively regulates Sf1 expression by antagonizing WT1-KTS during early ovarian development in mice and provide new mechanistic insight into the differentiation of ovaries, especially granulosa cell differentiation.
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Report
(4 results)
Research Products
(9 results)
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[Presentation] FOXL2 transcriptionally represses Sf1 expression by antagonizing WT1 during ovarian development in mice2015
Author(s)
Kei Takasawa, Kenichi Kashimada, Emanuele Pelosi, Masatoshi Takagi, Tomohiro Morio, Hiroshi Asahara, David Schlessinger, Shuki Mizutani, Peter Koopman
Organizer
7th International Symposium on the Biology of Vertebrate Sex Determination
Place of Presentation
USA, Hawaii, Kona
Year and Date
2015-04-13 – 2015-04-17
Related Report
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