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Effect of anti-Mullerian hormone on germ cells of maturing rat testis

Research Project

Project/Area Number 24591504
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pediatrics
Research InstitutionUniversity of Yamanashi

Principal Investigator

KENJI Ohyama  山梨大学, 総合研究部, 医学研究員 (80051861)

Co-Investigator(Renkei-kenkyūsha) OHTA Masanori  山梨大学, 総合研究部, 医学研究員 (80233146)
SANO Tomoaki  山梨大学, 総合研究部, 医学研究員 (80447705)
YAGASAKI Hideaki  山梨大学, 総合研究部, 助教 (00377540)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords抗ミュラー管ホルモン / 生殖細胞 / 精巣 / 精子形成 / 抗ミュラー管ホルモン受容体 / 精毋細胞 / Smad / セルトリ細胞 / 精母細胞 / 精子細胞 / 性成熟 / アンドロゲン受容体
Outline of Final Research Achievements

The expression of AMH and AMHR2 mRNA and protein was determined to clarify the effects of of AMH on germ cells. <Methods> Animal:SD rats, Germ cell isolation:collagenase digestion and percoll gradient, Quantitative real-time PCR:postnatal 5day-35day testes, In situ hybridization(Amh, Amhr2 mRNA):21D and 49D testes, Immunohistochemistry (AMH, AMHR2 antibody):10-49D testes. <Results> In situ hybridization of 21D testis, expressions of Amh and Amhr2 were observed in spermatocytes, but negative on spermatogonia. Immunohistochemical stainings of AMH and AMHR2 in 21D were observed in spermatocytes and Sertoli cells, but not spermatogonia. The staining of AMH in 49D was positive in spermatocytes and round spermatids. The staning of AMHR2 was positive in spermatocytes, round spermatids and elongated spermatids. These results indicate that AMH expressed in developing germ cells is crucial molecule to lead precise spermatogenesis by binding to AMHR2 in spermatocyte and spermatids.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (11 results)

All 2014 2013 2012

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (6 results) (of which Invited: 2 results)

  • [Journal Article] 性分化疾患の性自認に関する調査研究2013

    • Author(s)
      大山建司,深見真紀、緒方勤
    • Journal Title

      日本小児科学会雑誌

      Volume: 117 Pages: 1403-1408

    • NAID

      10031195068

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 性分化疾患の新たな知見と臨床現場での対応2013

    • Author(s)
      大山建司
    • Journal Title

      日本小児科学会雑誌

      Volume: 117 Pages: 1722-1727

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Expression of a Novel Sphingosine 1-Phosphate Receptor Motif Protein Gene in Maturing Rat Testes2012

    • Author(s)
      Khalid AM, Asano A, Hosaka YZ, Ohyama K, Ohta M, Yamano Y.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 76 Issue: 9 Pages: 1769-1773

    • DOI

      10.1271/bbb.120379

    • NAID

      130004137977

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Regulation of Rat Tetratricopeptide Repeat Domain 29 Gene Expression by Follicle-Stimulating Hormone2012

    • Author(s)
      Ohta M, Ohyama K, Asano A, Yokota s, Khalid AM, Yamano Y
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 76 Issue: 8 Pages: 1540-1543

    • DOI

      10.1271/bbb.120293

    • NAID

      130004137938

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Rat Stem-Cell Leukemia Gene Expression Increased during Testis Maturation2012

    • Author(s)
      Khalid AM, Asano A, Hosaka YZ, Ohta M, Ohyama K, Yamano Y.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 76 Issue: 11 Pages: 2118-2123

    • DOI

      10.1271/bbb.120503

    • NAID

      130004137862

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] ラット精巣における抗ミュラー管ホルモンとその受容体の発現変動2014

    • Author(s)
      大山建司,田辺良子、小林基章、太田正法
    • Organizer
      第48回日本小児内分泌学会
    • Place of Presentation
      アクトシティ浜松(静岡県浜松市)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 胎児期のアンドロゲン効果に異常をきたす性分化疾患患者の性同一性2013

    • Author(s)
      大山建司,深見真紀、緒方勤
    • Organizer
      第47回日本小児内分泌学会
    • Place of Presentation
      浅草ビューホテル(東京都台東区)
    • Related Report
      2013 Research-status Report
  • [Presentation] AMHの精巣機能に及ぼす影響2012

    • Author(s)
      大山建司、太田正法、田辺良子
    • Organizer
      第16回小児分子内分泌研究会
    • Place of Presentation
      グランドパーク小樽 ( 小樽市 )
    • Related Report
      2012 Research-status Report
  • [Presentation] AMHは精子形成を抑制する(優秀演題賞)2012

    • Author(s)
      大山建司、太田正法、田辺良子
    • Organizer
      第46回日本小児内分泌学会
    • Place of Presentation
      大阪国際会議場 ( 大阪府 )
    • Related Report
      2012 Research-status Report
  • [Presentation] 性分化疾患をめぐる医療、社会の状況2012

    • Author(s)
      大山建司
    • Organizer
      第11回日本遺伝看護学会
    • Place of Presentation
      清泉寮( 山梨県清里 )
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 性の分化とその異常2012

    • Author(s)
      大山建司
    • Organizer
      第7回群馬小児神経・内分泌代謝カンファレンス
    • Place of Presentation
      前橋テルサ ( 前橋市 )
    • Related Report
      2012 Research-status Report
    • Invited

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Published: 2013-05-31   Modified: 2019-07-29  

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