| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
I mainly analyzed the mechanism of liver-specific suppression of SCOT gene.
1)Liver specific suppression activity was not observed in human and mouse 3’non-coding region sequences when examined by a luciferase assay. 2)Using ChiP assay with RNA polymerase 2 antibody, transcriptional activity of SCOT hene regions were compared with that of GAPDH in mouse heart, kidney and liver tissues. SCOT transcription in the liver was much low comparing to that in the kidney and heart. These data suggest that SCOT gene expression is suppressed mainly at the transcriptional level in liver. I also confirmed that a missense mutation c.949G>A(D317N) caused exon 10 skipping in the mini-gene splicing experiment, indicating that this mutation functions as a splicing mutation rather than amissense mutation.
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