| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
We addressed whether the pathogenesis of CINCA syndrome, a congenital bone and joint disease characterized by epiphyseal overgrowth, could be recapitulated by using neural crest-derived chondroprogenitor differentiation from using patient-derived iPS cells. 3D chondrogenic assays showed mutant iPS cells produced significantly huge chondrogenous pellets than wild type iPS cells. Furthermore, in vivo chongrogenic assays by subcutaneous xenotranplation into immunodeficient mice showed that huge chondrogenenous tissues, consisting of immature chondroprogenitors, were obtained, which recapitulates the pathological condition of the syndrome.
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