Characterization of peptide fragment of neurosecretory protein VGF precursor (373-417) in the mouse brain

• Project/Area Number: 24591528
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Nippon Medical School
• Principal Investigator: TAKESHI Asano 日本医科大学, 医学部, 准教授 (70277490)
• Co-Investigator(Kenkyū-buntansha): OZAWA Hitoshi 日本医科大学, 医学研究科, 教授 (60169290)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 急性脳症 / 髄液 / ペプチド / 核内蛋白 / VGF / VGFペプチド / 脳内発現 / 脳症 / 脳内内分泌物質 / VGF precursor / peptide

Outline of Final Research Achievements

The aim of the investigation was to explore the characterization of peptide fragment of neurosecretory protein VGF precursor (373-417) in the brain. We made the antibodies against the peptide and stained the mouse and rat brain tissue. We found the antibodies recognized nuclear protein in the neural cells in cerebral cortex, alveus hippocamp, nigra by immunohistochemistry and western blot. These results suggested the VGF peptide might act as nuclear protein.