| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Outline of Final Research Achievements |
Here we demonstrate that p115RhoGEF, a guanine nucleotide exchange factor for Rho, directly interacts with a protein at the nuclear periphery to organize the nuclear envelope and transcriptional regulators and thus promote differentiation of human leukaemic HL60 cells. Translocation of p115RhoGEF from the cytoplasm to the nucleus, in response to all-trans retinoic acid is initiated upon its phosphorylation on the specific phosphorylation site. This novel p115RhoGEF signal into the nucleus differentiates leukaemic cells, accompanied by the canonical p115RhoGEF-RhoA-actin organization at the plasma membrane.
|
