Aging process of hematopoietic stem cells induced by transplantation

• Project/Area Number: 24591557
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Kagoshima University
• Principal Investigator: KAWANO Yoshifumi 鹿児島大学, 医歯(薬)学総合研究科, 教授 (20260680)
• Co-Investigator(Kenkyū-buntansha): OKAMOTO Yasuhiro 鹿児島大学, 医学部歯学部附属病院, 講師 (30398002)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 造血幹細胞 / 加齢 / 老化 / 造血細胞移植 / 転写因子 / 造血幹細胞移植 / CD34陽性細胞

Outline of Final Research Achievements

The purposes of this study were to establish the method of evaluating aging process in human hematopoietic stem cells (HSC) and to detect biological variations of HSC induced by HSC transplantation. We analyzed aging process of HSC with the use of CD34-positive cell population as a substitute for a single HSC. As a result, the ratio of Ikaros/CEPBPa, which are transcription factors of lymphoid and myeloid respectively of primitive HSC, can be used as an evaluation method for HSC variation at the age of between 15 and 45. Although the precision of this method should be improved with some modifications, aging process by HSC transplantation can be shown with this method.