Identification of specific markers in in kawasaki disease patients with coronary artery lesions and in treatment-resistant kawasaki disease patients by proteomics

• Project/Area Number: 24591561
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: Karasawa Rie 聖マリアンナ医科大学, 医学(系)研究科(研究院), 講師 (50434410)
• Co-Investigator(Renkei-kenkyūsha): YUDOH KAZUO 聖マリアンナ医科大学, 医学(系)研究科(研究院), 教授 (60272928)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 川崎病 / 抗血管内皮細胞抗体 / Peroxiredoxin 2 / プロテオミクス / 冠動脈病変 / 治療抵抗性 / 国際情報交換 / 抗血管内皮細胞抗体 (AECA)

Outline of Final Research Achievements

Candidate target antigens for anti-endothelial cell antibodies (AECA) specific to Kawasaki disease (KD) patients with coronary artery lesions and specific to treatment-resistant KD patients were detected by a proteomic approach, a combination of two-dimensional electrophoresis and Western blotting, respectively. We found that these specific AECA might be novel biomarkers, both in KD patients with coronary artery lesions and in treatment-resistant KD patients. Anti-Peroxiredoxin 2(Prx2) antibodies are among the AECA we detected in KD. Both anti-Prx2 antibodies and Prx2 protein increased inflammatory cytokine secretion from endothelial cells (ECs) and expression of adfesion molecule on ECs, which might result in endothelial injury. Furthermore this research identified Toll-like receptor 4 as the main receptor on ECs for Prx2.

Research Products

• [Int'l Joint Research] バッファロー大学(米国)
• [Journal Article] Validation of a new biomarker in patients with Kawasaki disease identified by proteomics. (2013)
  • Author(s): Karasawa E, Fujieda M, Ohta K, Yudoh K.
  • Journal Title: J Data Mining Genomics Proteomics Volume: 4 Issue: 01 Pages: 124-124
  • DOI: 10.4172/2153-0602.1000124
  • Peer Reviewed
• [Journal Article] Progress and Clinical Applications in Proteomics. (2012)
  • Author(s): Karasawa R
  • Journal Title: Journal of Data Mining in Genomics & Proteomics Volume: S1 Issue: 02 Pages: 1-6
  • DOI: 10.4172/2153-0602.s1-001
  • Peer Reviewed
• [Presentation] Novel Roles for Peroxiredoxin2 as Target Antigens for Anti-Endothelial Cell Antibodies (2014)
  • Author(s): Karasawa Rie
  • Organizer: Global Biotechnology Congress 2014
  • Place of Presentation: Boston, USA
  • Year and Date: 2014-06-16 – 2014-06-19
• [Presentation] Pathological Roles of Peroxiredoxin 2 in Vasculitis (2013)
  • Author(s): Karasawa Rie
  • Organizer: Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: Chiba, Japan
• [Presentation] Pathological Roles of the Anti-Oxidative Enzyme Peroxiredoxin 2 in Patients with Kawasaki Disease (2013)
  • Author(s): Karasawa Rie
  • Organizer: American College of Rheumatology
  • Place of Presentation: San Diego, USA
• [Presentation] 川崎病の病態形成における抗酸化酵素Peroxiredoxin2の機能的役割の解明 (2013)
  • Author(s): 唐澤里江
  • Organizer: 第33回 日本川崎病学会・学術集会
  • Place of Presentation: 富山
• [Presentation] Detection of Target Antigens for Anti-Endothelial Cell Antibodies in Patients with Kawasaki Disease (2013)
  • Author(s): Karasawa Rie
  • Organizer: HUPO 12th Annual World Congress
  • Place of Presentation: Yokohama, Japan
• [Presentation] Autoantibodies to peroxiredoxin in patients with systemic autoimmune diseases. (2012)
  • Author(s): 唐澤里江
  • Organizer: 第56回日本リウマチ学会
  • Place of Presentation: 東京