Clarification of pathophysiology of virus-associated acute encephalaopathy by evaluationg the function of tight junctions in vitro
Project/Area Number |
24591581
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Pediatrics
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Research Institution | Fukushima Medical University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
KAWASAKI Yukihiko 福島県立医科大学, 医学部, 准教授 (00305369)
橋本 浩一 福島県立医科大学, 医学部, 准教授 (50322342)
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Co-Investigator(Renkei-kenkyūsha) |
HASHIMOTO Koichi 福島県立医科大学, 医学部, 准教授 (50322342)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
|
Keywords | 脳症 / 血管内皮 / サイトカイン / タイトジャンクション / 脳炎 / HUVEC |
Outline of Final Research Achievements |
The mechanism of endothelial injury caused by cytokines in severe virus-associated acute encephalopathy(AE) remains unclear. We established a method for estimating endothelial injury caused by TNFα, proinflammatory cytokine in vitro. Human umbilical vein endothelial cell (HUVEC) monolayers were treated with TNF-α, subsequently transendothelial electric resistance (TER) and permeability were measured using a impedance spectroscopy(cellZscope®), and FITC-conjugated dextran, respectively. Moreover, TNFα-induced morphological changes in claudin-5, one of the tight junction proteins, were observed by immunofluorescent staining. The decrease in TER, the time to TER recovery, and the increase in permeability were all dependent on TNF-α concentration. Delocalization of claudin-5 was also observed after TNF-α treatment. These methods are useful to clarify the mechanism of AE and subsequent vascular endothelial cells injury, and aid in the deve1opment of treatment options.
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] Survey of subacute sclerosing panencephalitis in Japan.2012
Author(s)
Abe Y, Hashimoto K, Iinuma K, Ohtsuka Y, Ichiyama T, Kusuhara K, Nomura K, Mizuguchi M, Aiba H, Suzuki Y, Mizusawa H, Hosoya M.
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Journal Title
J Child Neurol
Volume: 27
Issue: 12
Pages: 1529-1533
DOI
Related Report
Peer Reviewed
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