Molecular model of accumulation of multiple mutations on the way of transition of a measles virus to a subacute sclerosing panencephalitis virus
Project/Area Number |
24591589
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Pediatrics
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Research Institution | Nagahama Institute of Bio-Science and Technology |
Principal Investigator |
ITOH Masae 長浜バイオ大学, バイオサイエンス学部, 教授 (10201328)
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Co-Investigator(Kenkyū-buntansha) |
HOTTA Hak 神戸大学, 大学院医学研究科, 教授 (40116249)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | 麻疹ウイルス / SSPEウイルス / 神経病原性 / 細胞融合 / Fusion蛋白 |
Outline of Final Research Achievements |
Analysis of the accumulated multiple mutations was performed on the genome of a subacute sclerosing panencephalitis (SSPE) virus Kobe-1 strain isolated from a 5-year-old patient 6 weeks after the disease onset with the aim to elucidate the molecular mechanism underlying SSPE development. Virus cell-cell fusion ability was closely related with its neuropathogenicity, for which mutations not only on the F protein but also on the M protein were indispensable. Mutations on the H protein enhanced the pathogenicity. On the other hand we successfully identified some mutations on the F and H proteins that suppress virus cell-cell fusion and as a result restrict virus replication. These suppressive mutations were considered to be involved in the considerably long-time persistent infection of a measles virus in the brain of the patient before the measles virus acquires neuropathogenicity and becomes SSPE virus.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Intramolecular complementation of measles virus fusion protein stability confers cell-cell fusion activity at 37 °C.2015
Author(s)
Satoh, Y., Hirose, M., Shogaki, H., Wakimoto, H., Kitagawa, Y., Gotoh, B., Takahashi, K. and Itoh, M.
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Journal Title
FEBS Lett.
Volume: 589
Issue: 1
Pages: 152-158
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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