| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Through genetic analyses of the revertant mumps virus (MuV), we revealed that the main cause of the reversion is the mutation in the V/P gene. Meanwhile,introducing the mutation of the L gene of the highly virulent strain has enhanced the neurovirulence of an attenuated MuV. In addition, the MuV isolates from vaccinal horizontal transmission cases habor the characteristic mutations in their L genes. And their neurovirulence were enhanced comparing with the original vaccine viruses. In order to assess the involvement of L gene in MuV neurovirulence, we constructed recombinant MuV (rOdate/miR) which has the L gene harboring the complement sequences of the miRNA expressed specifically in central nerve system. As the result, rOdate/miR exhibited little neurovirulence.
These results strongly suggested that the main causative genes for MuV neurovirulence are V/P and L genes.
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