Research on the molecular basis for virulence expression in mumps virus
Project/Area Number |
24591592
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Pediatrics
|
Research Institution | National Institute of Infectious Diseases |
Principal Investigator |
KIDOKORO MINORU 国立感染症研究所, ウイルス第三部第三室, 室長 (00370958)
|
Research Collaborator |
KATOH Hiroshi 国立感染症研究所, ウイルス第三部第三室, 研究員 (80711712)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | ムンプスウイルス / 中枢神経病原性 / リバースジェネティクス / 病原性復帰 / V/P遺伝子 / L遺伝子 |
Outline of Final Research Achievements |
Through genetic analyses of the revertant mumps virus (MuV), we revealed that the main cause of the reversion is the mutation in the V/P gene. Meanwhile,introducing the mutation of the L gene of the highly virulent strain has enhanced the neurovirulence of an attenuated MuV. In addition, the MuV isolates from vaccinal horizontal transmission cases habor the characteristic mutations in their L genes. And their neurovirulence were enhanced comparing with the original vaccine viruses. In order to assess the involvement of L gene in MuV neurovirulence, we constructed recombinant MuV (rOdate/miR) which has the L gene harboring the complement sequences of the miRNA expressed specifically in central nerve system. As the result, rOdate/miR exhibited little neurovirulence. These results strongly suggested that the main causative genes for MuV neurovirulence are V/P and L genes.
|
Report
(4 results)
Research Products
(10 results)