Development of detection of EBV infection and monitoring of immunosuppression by molecular analysis after pediatric liver trasplantation

• Project/Area Number: 24591593
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: FUKUDA Akinari 独立行政法人国立成育医療研究センター, その他部局等, その他 (60455417)
• Co-Investigator(Kenkyū-buntansha): IMADOME Ken-ichi 国立成育医療研究センター, 母児感染研究部, 室長 (70392488) ; UMEZAWA Akihiro 国立成育医療研究センター, 生殖・細胞医療研究部, 部長 (70213486) ; KASAHARA Mureo 国立成育医療研究センター・臓器移植センター, センター長 (30324651) ; SAKAMOTO Seisuke 国立成育医療研究センター・臓器移植センター (00378689)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: EBウィルス / 低侵襲検査法 / 免疫抑制療法 / 小児肝移植 / 細胞障害性T細胞活性 / EBVウィルス

Outline of Final Research Achievements

The immune function assay is a measure of cell-mediated immunity based on the peripheral CD4+ T cell adenosine triphosphate (ATP) activity. The aim of this study was to assess the correlations between the CD4+ T cell ATP activity assay and the clinical status in pediatric LDLT recipients. The recipient’s status was classified as follows: stable, infection or rejection.The CD4+ T cell ATP activity in the pediatric LDLT recipients with a clinically stable status had a lower immune response range (median 162 [interquartile range: IQR, 85-297] ATPng/ml).the correlations between the CD4+ T cell ATP activity and the status of rejection or EBV viremia were limited. We developed the method of isolation of EBV from saliva.

Research Products

• [Journal Article] Evaluation of the Immune Function Assay in Pediatric Living Donor Liver Transplantation (2015)
  • Author(s): Akinari Fukuda, Ken-Ichi Imadome, Seisuke Sakamoto, Takanobu Shigeta, Hajime Uchida, Masatoshi Matsunami, Kengo Sasaki, Hiroyuki Kanazawa, Fuyuko Kawano, Atsuko Nakazawa, Shigeyoshi Fujiwara, and Mureo Kasahara
  • Journal Title: Pediatric Transplantation Volume: 19(2) Issue: 2 Pages: 144-152
  • DOI: 10.1111/petr.12402
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 小児肝移植後EBウイルス感染症への対策 (2014)
  • Author(s): 福田 晃也, 今留 謙一, 笠原 群生
  • Journal Title: 今日の移植 Volume: 27(4) Pages: 271-278
• [Journal Article] 小児肝移植後EB ウイルス感染症への対策 (2014)
  • Author(s): 福田晃也，笠原群生
  • Journal Title: 今日の移植 Volume: 47
• [Journal Article] Effective control of Epstein-Barr virus infection following pediatric liver transplantation by monitoring of viral DNA load and lymphocyte surface markers (2012)
  • Author(s): Imadome K, Fukuda A
  • Journal Title: Pediatric Transplantation Volume: 16(Epub ahead of print) Issue: 7 Pages: 748-757
  • DOI: 10.1111/j.1399-3046.2012.01750.x
  • Peer Reviewed
• [Presentation] 小児生体肝移植後Epstein-Barr virus感染に対するアシクロビル予防投与の効果の検討 (2014)
  • Author(s): 福田晃也，今留謙一，阪本靖介，佐々木健吾，内田 孟，濱野郁美，重田孝信，金澤寛之，中澤温子，笠原群生
  • Organizer: 第114回日本外科学会定期学術集会
  • Place of Presentation: 国立京都国際会館，京都市
• [Presentation] Prevention of Epstein-Barr virus infection after pediatric liver transplantation (2013)
  • Author(s): Akinari Fukuda, Ken-ichi Imadome, Seisuke Sakamoto, Kengo Sasaki, Hajime Uchida, Ikumi Hamano, Takanobu Shigeta, Hiroyuki Kanazawa, Atsuko Nakazawa, Mureo Kasahara, Akira Matsui
  • Organizer: 13th Asian Pan-Pacific Society for Pediatric Gastroenterology, Hepatology and Nutrition
  • Place of Presentation: Tokyo, Japan
• [Presentation] 小児肝移植後におけるCD4陽性リンパ球ATP活性の推移についての検討 (2013)
  • Author(s): 福田晃也
  • Organizer: 第14回 肝移植術後管理検討会
  • Place of Presentation: 京都市
• [Presentation] 小児肝移植後のEBウィルス感染症に対する対策 (2013)
  • Author(s): 福田晃也
  • Organizer: 第49回 日本移植学会
  • Place of Presentation: 京都市