| Project/Area Number |
24591612
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Showa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SIODA Seiji 昭和大学, 医学部・顕微解剖学教室, 教授 (80102375)
SHIBATO Junko 昭和大学, 医学部・顕微解剖学教室, 研究員 (10611121)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | DOHaD / 網羅的遺伝子解析 / マウス / 新生児期栄養環境変化 / 自然免疫 / 遺伝子解析 / 新生児期栄養環境 / 発達障害 |
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| Outline of Final Research Achievements |
Although undernutrition during gestation is harmful for fetuses, calorie restriction is a strategy proven to extend healthy and maximum life span after birth. By focusing our attention on genes whose expression oppositely regulated between under- or high-nutrition during neonatal period, we selected candidate genes responsible for DOHaD. The results of microarray in C57BL mouse pup’s liver exposed to neonatal under- (50% food restriction) and high-nutrition (60% high fat food) demonstrated that innate immune system (Fos、Il1b、Rgs1、Ppp1r3g、Hamp、Igll1) was activated shortly after exposure. At weaning, expression of Aldhla 7 was decreased resulting of low resolution of acetaldehyde regardless of the nutritional conditions. By histopathological examination, abnormal development of immune tissues such as thymus and spleen was detected.
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