The exhaustive search of foreign DNAs as the pathogenic factor of angiosarcoma.
| Project/Area Number |
24591631
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
TAKAHASHI Kenzo 琉球大学, 医学(系)研究科(研究院), 准教授 (80291425)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 血管肉腫 / 発癌ウイルス / トランスクリプトーム解析 / ヘルペスウイルス / ウイルス発癌 / サブトラクション / カポシ肉腫 / ウイルス / 発がん / 外来遺伝子 |
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| Outline of Final Research Achievements |
We planned to look for the gene fragment of pathogenic virus for hemangiosarcoma, which occur frequently in Okinawa prefecture. Although the gene sequences common to zootrophic herpesvirus group were chosen and used for PCR amplification with various annealing temperatures, the herpesvirus of known or a de novo was not amplified at all.
The presence of any foreign gene was not detected by the subtraction screening. Only the gene sequences of the organism those were considered to have no intervention in pathogenesis, such as a demodex, normal cutaneous inhabitant, or candida were found to be transcribed, even though RNA was directly extracted from angiosarcoma tissue and their transcriptomes were determined exhaustively.
Thus, we could not come to determine the existence of the foreign microbes those might lead to the onset of hemangiosarcoma.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] CD8+ tumor-infiltrating lymphocytes at primary sites as a possible prognostic factor of cutaneous angiosarcoma2014
Author(s)
Fujii H, Arakawa A, Utsumi D, Sumiyoshi S, Yamamoto Y, Kitoh A, Ono M, Matsumura Y, Kato M, Konishi K, Shiga T, Sano S, Sakaguchi S, Miyagawa-Hayashino A, Takahashi K, Uezato H, Miyachi Y, Tanioka M.
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Journal Title
Int J Cancer
Volume: 134
Issue: 10
Pages: 2393-2402
DOI
NAID
Related Report
Peer Reviewed / Open Access
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