• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The novel mechanisms for the regulation of melanogenesis

Research Project

Project/Area Number 24591641
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Dermatology
Research InstitutionYamagata University

Principal Investigator

KAWAGUCHI MASAKAZU  山形大学, 医学部, 講師 (10302291)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsメラノサイト / メラノソーム / PMEL17 / diacylglycerol kinase / ADAM protease / 色素細胞 / melanosome / DGK / メラニン / ADAM / pmel17 / メラニン合成 / 紫外線
Outline of Final Research Achievements

ADAMs (a disintegrin and metalloprotease) are a family of proteases involved in ectodomain shedding that play a role in various biological processes such as cell adhesion and migration. We examined the effect of ADAM protease inhibitors on the modulation of melanogenesis in normal human epidermal melanocytes (NHEM). In NHEMs, melanin content was reduced by treatment with ADAM protease inhibitors. Electron microscopy showed that the number of fibrillar and mature melanosomes was significantly reduced and that the vacuolar compartments were filled with dense unstructured aggregates after treatment with ADAM protease inhibitors. We therefore focused on the processing of PMEL17, a melanosomal glycoprotein that forms a fibrillar matrix on which melanin gets deposited. We found that ADAM protease inhibitors exerted effects on the processing of C-terminal and N-terminal fragments of PMEL17.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (8 results)

All 2015 2014 2013 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (7 results) (of which Invited: 1 results)

  • [Journal Article] ADAM protease inhibitors reduce melanogenesis by regulating PMEL17 processing in human melanocytes2015

    • Author(s)
      Kawaguchi M, Hozumi Y, Suzuki T
    • Journal Title

      Journal of Dermatological Science

      Volume: 78 Issue: 2 Pages: 133-142

    • DOI

      10.1016/j.jdermsci.2015.02.020

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] The role of ADAM proteases on the processing of pmel172014

    • Author(s)
      Masakazu Kawaguchi, Yutaka Hozumi, Tamio Suzuki
    • Organizer
      第39回日本研究皮膚科学会
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪市)
    • Year and Date
      2014-12-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] ADAM protease inhibitor modulates melanogenesis in human melanocytes2014

    • Author(s)
      Masakazu Kawaguchi, Yutaka Hozumi, Tamio Suzuki
    • Organizer
      12th international pigment cell conference
    • Place of Presentation
      Shangri-La Hotel (Singapore)
    • Year and Date
      2014-09-04 – 2014-09-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] ADAM protease inhibitor regulates chemokine expression in human keratinocytes, and modulates melanogenesis in human melanocytes2013

    • Author(s)
      Masakazu Kawaguchi, Yutaka Hozumi, Tamio Suzuki
    • Organizer
      International Investigative Dermatology
    • Place of Presentation
      Edinburgh International Conference Centre (Edinburgh, Scotland)
    • Related Report
      2013 Research-status Report
  • [Presentation] 色素沈着のメカニズム

    • Author(s)
      川口雅一
    • Organizer
      第112回日本皮膚科学会総会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] ADAMプロテアーゼ阻害剤はヒトメラノサイトのmelanogenesisを調節する

    • Author(s)
      川口雅一、穂積 豊、鈴木民夫
    • Organizer
      第25回日本色素細胞学会学術大会
    • Place of Presentation
      大阪大学吹田キャンパス 銀杏会館(大阪市)
    • Related Report
      2013 Research-status Report
  • [Presentation] Diacylglycerol kinaseはメラノサイトのtyrosinase発現と機能を調節する

    • Author(s)
      川口雅一、Julio C. Valencia、Takeshi Namiki、鈴木民夫、Vincent J. Hearing
    • Organizer
      第24回日本色素細胞学会
    • Place of Presentation
      長浜バイオ大学(長浜市)
    • Related Report
      2012 Research-status Report
  • [Presentation] Diacylglycerol kinase regulates tyrosinase expression and function in human melanocytes

    • Author(s)
      Masakazu Kawaguchi, Julio C. Valencia, Takeshi Namiki, Tamio Suzuki, Vincent J. Hearing
    • Organizer
      第37回日本研究皮膚科学会
    • Place of Presentation
      パシフィックホテル沖縄(那覇市)
    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi