Development of dermatitis therapeutic drug with thioredoxin and thioredoxin inducer

• Project/Area Number: 24591652
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Kobe University
• Principal Investigator: FUKUNAGA Atsushi 神戸大学, 医学部附属病院, 講師 (10467649)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 皮膚炎症 / アトピー性皮膚炎 / チオレドキシン / 接触皮膚炎 / 外用療法

Outline of Final Research Achievements

The central medical therapy of eczema including atopic dermatitis has been both topical steroid and topical immunosuppressant for many years. In this study, we focused on thioredoxin (TRX), which has a crucial role in redox regulation. We demonstrated that topically applied recombinant human TRX (rhTRX) ameliorated a murine irritant contact dermatitis by regulating the epithelial production of pro-Inflammatory cytokines. We made an external agent containing rhTRX, which did not show allergic or irritant reaction in healthy volunteers. We have completed the double-blinded, randomized, placebo-controlled study in 20 patients with atopic dermatitis. Any side effects and harmful phenomenon did not occur in this study. This data are being analyzed.

Research Products

• [Journal Article] Eluted substances from hemodialysis membranes elicit positive skin prick tests in bio-incompatible patients. (2015)
  • Author(s): Konishi S, Fukunaga A, Yamashita H, Miyata M, Usami M.
  • Journal Title: Artif Organs. Volume: 39 Issue: 4 Pages: 343-351
  • DOI: 10.1111/aor.12392
  • Peer Reviewed
• [Journal Article] Combined cholinergic urticaria and cold-induced cholinergic urticaria with acquired idiopathic generalized anhidrosis (2015)
  • Author(s): Oda Y, Fukunaga A, Tsujimoto M, Hatakeyama M, Washio K, Nishigori C.
  • Journal Title: Allergology International Volume: 64 Issue: 2 Pages: 214-215
  • DOI: 10.1016/j.alit.2014.12.004
  • NAID: 130005290255
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed
• [Journal Article] Steroid treatment can improve the impaired quality of life of patients with acquired idiopathic generalized anhidrosis. (2015)
  • Author(s): Fukunaga A, Hatakeyama M, Tsujimoto M, Oda Y, Washio K, Nishigori C.
  • Journal Title: Br J Dermatol. Volume: 172 Issue: 2 Pages: 537-538
  • DOI: 10.1111/bjd.13285
  • Peer Reviewed
• [Journal Article] Epstein-Barr virus associated methotrexate-induced accelerated rheumatoid nodulosis. (2014)
  • Author(s): Shimoura, N., Fukunaga, A., Nagai, H., Oka, M., Nishigori, C.
  • Journal Title: Acta Derm. Venereol. Volume: - Pages: 100-101
  • DOI: 10.2340/00015555-1866
  • NAID: 120005723183
  • Peer Reviewed
• [Journal Article] Hypohidrosis plays a crucial role in the vicious circle of bathing suit ichthyosis: a case with summer exacerbation. (2014)
  • Author(s): Washio K, Fukunaga A, Terai M, Hitomi K, Yamanishi K, Nishigori C.
  • Journal Title: Acta Derm Venereol. Volume: 94 Issue: 3 Pages: 349-350
  • DOI: 10.2340/00015555-1739
  • NAID: 120005723195
  • Peer Reviewed
• [Journal Article] Thioredoxin ameliorates cutaneous inflammation by regulating the epithelial production and release of pro-Inflammatory cytokines (2013)
  • Author(s): Tian H, Matsuo Y, Fukunaga A, Ono R, Nishigori C, Yodoi J.
  • Journal Title: Front Immunol. Volume: 4 Pages: 269-269
  • DOI: 10.3389/fimmu.2013.00269
  • Peer Reviewed
• [Journal Article] Suppressive effect of administration of human recombinant thioredoxin on ultrviolet light-induced inflammation and apoptosis of murine skin. (2013)
  • Author(s): Ono R, Fukunaga A, Masaki T, Yu X, Yodoi J, Nishigori C.
  • Journal Title: Bioengineered. Volume: 4 Issue: 4 Pages: 254-257
  • DOI: 10.4161/bioe.23612
  • Peer Reviewed
• [Journal Article] Onychomycosis as a warning sign for peripheral arterial disease. (2013)
  • Author(s): Fukunaga, A., Washio, K., Ogura, K., Taguchi, K., Chiyomaru, K., Ohno, Y., Masaki, T., Nagai, H., Nagano, T., Oka, M., Nishigori, C.
  • Journal Title: Acta Derm. Venereol. Volume: 93 Issue: 6 Pages: 747-748
  • DOI: 10.2340/00015555-1576
  • NAID: 120005723197
  • Peer Reviewed
• [Journal Article] The role of epidermal Langerhans cells in NB-UVB-induced immunosuppression. (2013)
  • Author(s): Taguchi K, Fukunaga A, Ogura K, Nishigori C.
  • Journal Title: Kobe J Med Sci Volume: 59
  • NAID: 110009595357
  • Peer Reviewed
• [Journal Article] A case of multiple facial clear cell acanthoma.successfully treated with cryotherapy. (2013)
  • Author(s): Hatakeyama, A., Oka, M., Fukunaga, A., Kunisada, M., Funasaka, Y., Nishigori, C.
  • Journal Title: Indian J. Dermatol. Volume: 58 Issue: 2 Pages: 162-162
  • DOI: 10.4103/0019-5154.108122
  • Peer Reviewed
• [Journal Article] Unilateral aquagenic wrinkling of the palms with a peculiar clinical course. (2012)
  • Author(s): Ibusuki, C., Oka, M., Fukunaga, A., Kunisada, M., Nishigori, C.
  • Journal Title: Eur. J. Dermatol. Volume: 22 Issue: 5 Pages: 679-680
  • DOI: 10.1684/ejd.2012.1792
  • Peer Reviewed
• [Journal Article] Suppressive effect of recombinant human thioredoxin on ultraviolet light-induced inflammation and apoptosis in murine skin. (2012)
  • Author(s): Ono R, Masaki T, Dien S, Yu X, Fukunaga A, Yodoi J, Nishigori C.
  • Journal Title: J Dermatol. Volume: 39 Issue: 10 Pages: 843-851
  • DOI: 10.1111/j.1346-8138.2012.01566.x
  • Peer Reviewed
• [Journal Article] Successful and long-lasting treatment of solar urticaria with UVA rush hardening therapy (2012)
  • Author(s): Masuoka E, Fukunaga A, Kishigami K, Jimbo H, Nishioka M, Uchimura Y, Taguchi K, Ohgou N, Nishigori C.
  • Journal Title: Br J Dermatol. Volume: 167 Issue: 1 Pages: 198-201
  • DOI: 10.1111/j.1365-2133.2012.10944.x
  • Peer Reviewed
• [Journal Article] The Rho kinase pathway regulates the migration of dendritic cells through SIRP-α (2012)
  • Author(s): Ogura K, Fukunaga A, Taguchi K, Nagai H, Yu X, Oniki S, Okazawa H, Matozaki T, Horikawa T, Nishigori C
  • Journal Title: J Dermatol Sci Volume: 66 Issue: 1 Pages: 74-6
  • DOI: 10.1016/j.jdermsci.2012.02.003
  • Peer Reviewed
• [Journal Article] Drug fever due to S-carboxymethyl-L-cysteine : The demonstrati on of a causative agent with patch tests (2011)
  • Author(s): Hatakeyama M, Fukunaga A, Shimizu H, Oka M, Horikawa T, Nishigori C
  • Journal Title: J Dermatol Volume: (Epub ahead of print) Issue: 6 Pages: 555-556
  • DOI: 10.1111/j.1346-8138.2011.01325.x
  • Peer Reviewed
• [Journal Article] Limited influence of aspirin intake on mast cell activation in patients with food-dependent ecercise-induced anaphylaxis : comparison using skin prick and histamine release tests (2011)
  • Author(s): Fukunaga A, Shimizu H, Tanaka M, Kikuzawa A, Tsujimoto M, Sekimukai A, Yamashita J, Horikawa T, Nishigori C
  • Journal Title: Ada Derm Venereol Volume: (Epub ahead of print) Issue: 5 Pages: 480-483
  • DOI: 10.2340/00015555-1210
  • Peer Reviewed
• [Presentation] 専門医試験に必要なアレルギー・光線検査 (2014)
  • Author(s): 福永 淳
  • Organizer: 第65回日本皮膚科学会中部支部学術大会
  • Place of Presentation: 大阪
  • Year and Date: 2014-10-25 – 2014-10-26
  • Invited
• [Presentation] 日光蕁麻疹、寒冷蕁麻疹に対するhardening治療 (2014)
  • Author(s): 福永 淳
  • Organizer: 第113回日本皮膚科学会総会・学術大会
  • Place of Presentation: 京都
  • Year and Date: 2014-05-30 – 2014-06-01
  • Invited
• [Presentation] Thioredoxin Suppresses Irritant Dermatitis to Croton Oil via the Inhibition of Cytokines and Chemokines Produced by Keratinocytes. (2012)
  • Author(s): Hai Tian, Atsushi Fukunaga*, Kumiko Taguchi*, Susumu Fujiwara*, Hiroshi Nagai*, Yoshiuki Matsuo, Junji Yodoi, Chikako Nishigori*
  • Organizer: 日本研究皮膚科学会第37回年次学術大会・総会
  • Place of Presentation: 沖縄