| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Observation of real-timely cell dynamics is needed for evaluation of trichogenesis. In this study, I developed long-term cultivation method of two type cells, epithelial stem cells (EpSCs) and dermal papilla cells (DPCs), which are essential for trichogenesis by using Wnt proteins. EpSCs proliferated markedly in the presence of Wnt-3a, maintained undifferentiated epithelial cell marker expression, and promoted hair follicle development in vivo. DPCs cultured with Wnt-10b were promoted their proliferation and maintained their hair follicle induction ability. These results strongly suggested the important roles of specifical Wnts in the maintenance of EpSCs and DPCs. Furthermore, mixtures of EpSCs and DPCs differentiated into the structures with immature hair in vitro.
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