Elucidation of trichogenous using in vitro hair-culture system
| Project/Area Number |
24591661
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Dermatology
|
|---|
| Research Institution | Nara Medical University |
|---|
Principal Investigator |
OUJI Yukiteru 奈良県立医科大学, 医学部, 講師 (50343421)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 発毛 / Wntシグナル / 幹細胞 / 上皮 / 毛乳頭 / 再生医学 / 細胞移植 / 発毛再生 / 皮膚再生 / Wnt / 毛包 / 細胞培養 |
|---|
| Outline of Final Research Achievements |
Observation of real-timely cell dynamics is needed for evaluation of trichogenesis. In this study, I developed long-term cultivation method of two type cells, epithelial stem cells (EpSCs) and dermal papilla cells (DPCs), which are essential for trichogenesis by using Wnt proteins. EpSCs proliferated markedly in the presence of Wnt-3a, maintained undifferentiated epithelial cell marker expression, and promoted hair follicle development in vivo. DPCs cultured with Wnt-10b were promoted their proliferation and maintained their hair follicle induction ability. These results strongly suggested the important roles of specifical Wnts in the maintenance of EpSCs and DPCs. Furthermore, mixtures of EpSCs and DPCs differentiated into the structures with immature hair in vitro.
|
Report
(4 results)
Research Products
(25 results)
-
-
[Journal Article] Cytotoxic human peripheral blood-derived γδT cells kill glioblastoma cell lines: implications for cell-based immunotherapy for patients with glioblastoma2014
Author(s)
Nakazawa T, Nakamura M, Park YS, Motoyama Y, Hironaka Y, Nishimura F, Nakagawa I, Yamada S, Matsuda R, Tamura K, Sugimoto T, Takeshima Y, Marutani A, Tsujimura T, Ouji N, Ouji Y, Yoshikawa M, Nakase H
-
Journal Title
J Neurooncol
Volume: 116
Issue: 1
Pages: 31-39
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
