| Project/Area Number |
24591672
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Kurume University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HASHIMOTO Takashi 久留米大学, 皮膚細胞生物学研究所, 教授 (20129597)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | メラニン生成 / メラノサイト / 転写因子 / クロマチン免疫沈降法 / 次世代シーケンシング / シグナル伝達解析 |
|---|
| Outline of Final Research Achievements |
Ubiquitous MITF-related transcription factors TFEB, TFE3 and TCF4 were found to be significantly over-expressed in cultured melanocytes when the cAMP level was suppressed below steady state. To discover relevant functional miRNA-transcription factor relationships, differentially expressed miRNAs were screened by miRNA microarray. Three miRNA, miR-30b, -146a, and -29a, including miRNA known to interact with these transcription factors, were found to be over-expressed. To explore genome-wide binding sites of TCF4 transcription factor, genome-wide ChIP-seq was performed on cultured melanocytes with seven anti-TCF4 antibodies. However, even though in the entire chromosome views the TCF4 data show more peaks than the negative control, all seven TCF4-specific antibody validation results appeared to be negative, since targeted deep sequencing reveals no significant results.
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