Behavioral and pharmacological studies for the purpose of function analysis of transcription factors in brain dopamine system

• Project/Area Number: 24591684
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Psychiatric science
• Research Institution: University of Miyazaki
• Principal Investigator: ISHIDA Yasushi 宮崎大学, 医学部, 教授 (20212897)
• Co-Investigator(Kenkyū-buntansha): TAKEDA Ryuichiro 宮崎大学, 安全衛生保健センター, 教授 (90336298) ; ABE Hiroshi 宮崎大学, 医学部, 講師 (20344848) ; NISHIMORI Toshikazu 宮崎大学, 医学部, 研究員 (20112211)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: パーキンソン病 / 神経移植 / 転写調節因子 / ドパミン / 行動薬理学

Outline of Final Research Achievements

A study was carried out to examine the effects of repeated L-DOPA treatment on the distribution of the immediate-early gene (IEG) proteins (FosB, c-Fos, and Zif268) in forebrain regions in a unilateral 6-hydroxydopamine (6-OHDA) rat model of Parkinson’s disease with or without intrastriatal grafts of fetal ventral mesencephalon. During a course of repeated L-DOPA treatment, rats with a 6-OHDA lesion developed abnormal involuntary movements. In summary, differential expression of three IEG proteins was observed in the forebrain regions during a course of repeated L-DOPA treatment of 6-OHDA-treated hemiparkinsonian rats.
The other goal of this study was to investigate the effect of intrastriatal grafts of fetal ventral mesencephalon (VM) on the withdrawal response in 6-OHDA rats. The present results suggest that the intrastriatal grafts of fetal VM may relieve pain sensation induced by mechanical stimulation in 6-OHDA rats.

Research Products

• [Journal Article] Pramipexole reduces parkinsonian tremor induced by pilocarpine infusion in the rat striatum (2015)
  • Author(s): Yasuda K, Abe H, Koganemaru G, Ikeda T, Arimori K, Ishida Y
  • Journal Title: Pharmacol Biochem Behav Volume: 131 Pages: 1-5
  • DOI: 10.1016/j.pbb.2015.01.012
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Hemokinin-1-derived peptides have antipruritic effects in rats. (2015)
  • Author(s): H. Funahashi, R.Naono-Nakayama, G. Koganemaru, Y. Miyahara, T. Nishimori, K. Takamiya, Y. Ishida
  • Journal Title: Therapeutic Targets for Neurological Diseases Volume: 2 Pages: 1-4
  • DOI: 10.14800/ttnd.704
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Intrastriatal grafts of fetal ventral mesencephalon improve allodynia-like withdrawal response to mechanical stimulation in a rat model of Parkinson's disease (2014)
  • Author(s): Takeda R, Ishida Y, Ebihara K, Abe H, Matsuo H, Ikeda T, Koganemaru G, Kuramashi A, Funahashi H, Magata Y, Kawai K, Nishimori T
  • Journal Title: Neurosci Lett Volume: 573 Pages: 19-23
  • DOI: 10.1016/j.neulet.2014.05.007
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The role of spinal serotonin receptor and alpha adrenoceptor on the antiallodynic effects induced by intrathecal milnacipran in chronic constriction injury rats. (2014)
  • Author(s): T. Nakamura, T. Ikeda, R. Takeda, K. Igawa, R. Naono-Nakayama, S. Sakoda, T. Nishimori, Ishida Y
  • Journal Title: Eur J Pharmacol. Volume: 738 Pages: 57-65
  • DOI: 10.1016/j.ejphar.2014.05.022
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Hemokinin-1 mediates pruriceptive processing in the rat spinal cord. (2014)
  • Author(s): H. Funahashi, R.Naono-Nakayama, K. Ebihara, G. Koganemaru, A. Kuramashi, T. Ikeda,T. Nishimori, Y. Ishida
  • Journal Title: Neuroscience Volume: 277 Pages: 206-216
  • DOI: 10.1016/j.neuroscience.2014.07.001
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effects of cabergoline and rotigotine on tacrine-induced tremulous jaw movements in rats (2014)
  • Author(s): Koganemaru G, Abe H, Kuramashi A, Nishimori T, Ebihara K, Matsuo H, Funahashi H, Yasuda K, Ikeda T, Ishida Y
  • Journal Title: Pharmacol Biochem Behav Volume: 126 Pages: 103-108
  • DOI: 10.1016/j.pbb.2014.09.015
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The role of spinal serotonin receptor and alpha adrenoceptor on the antiallodynic effects induced by intrathecal milnacipran in chronic constriction injury rats (2014)
  • Author(s): Nakamura T, Ikeda T, Takeda R, Igawa K, Naono-Nakayama R, Sakoda S, Nishimori T, Ishida Y
  • Journal Title: European Journal of Pharmacology Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Effect of blonanserin on methamphetamine-induced disruption of latent inhibition and c-Fos expression in rats (2013)
  • Author(s): Kuramashi A, Abe H, Koganemaru G, Matsuo H, Ikeda T, Ebihara K, Funahashi H, Takeda R, Nishimori T, Ishida Y
  • Journal Title: Neuroscience Letters Volume: 549 Pages: 97-102
  • DOI: 10.1016/j.neulet.2013.06.020
  • Peer Reviewed
• [Journal Article] Functional dissociation between rat perirhinal cortex and hippocampus in object and place discrimination tasks（私の論文から） (2012)
  • Author(s): 安部博史，石田康
  • Journal Title: 九州神経精神医学 Volume: 58 Pages: 62-68
• [Presentation] Involvement of immune protein mhc class I in the development of cocaine self-administration (2014)
  • Author(s): Murakami G, Meng H, Edamura M, Furukawa T, Fukuda A, Iwashita T, Ishida Y, Nakahara D
  • Organizer: 44th Annual Meeting Society for Neuroscience
  • Place of Presentation: Washington, DC（米国）
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] Hemokinin-1 mediates pruriceptive processing in the rat spinal cord (2014)
  • Author(s): Funahashi H, Naono-Nakayama R, Abe H, Ebihara K, Nishimori T, Ishida Y
  • Organizer: 44th Annual Meeting Society for Neuroscience
  • Place of Presentation: Washington, DC（米国）
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] Effect of an N-methyl-D-aspartate receptor antagonist in animal models of schizophrenia: An immunohistochemical study in the rat (2014)
  • Author(s): Matsuo H, Nishimori T, Abe H, Kuramashi A, Koganemaru G, Funahashi H, Ebihara K, Ikeda T, Ishida Y
  • Organizer: 44th Annual Meeting Society for Neuroscience
  • Place of Presentation: Washington, DC（米国）
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] セロトニン産生細胞RIN-14BにおけるL-DOPA誘発ドパミン合成に対する抑肝散の増強作用 (2014)
  • Author(s): 今村幸子，田渕雅宏，山口琢児，五十嵐康，加瀬義夫，蛯原功介，石田康
  • Organizer: 第36回日本生物学的精神医学会・第57回日本神経化学会大会
  • Place of Presentation: 奈良県文化会館・奈良県新公会堂（奈良県奈良市）
  • Year and Date: 2014-09-29 – 2014-10-01
• [Presentation] 統合失調症の潜在抑制障害モデルを用いた、ブロナンセリンの改善効果に関する行動学的、形態学的検討 (2014)
  • Author(s): 倉増亜紀，安部博史，小金丸剛，松尾寿栄，池田哲也，蛯原功介，船橋英樹，武田龍一郎，西森利數，石田康
  • Organizer: 第36回日本生物学的精神医学会・第57回日本神経化学会大会
  • Place of Presentation: 奈良県文化会館・奈良県新公会堂（奈良県奈良市）
  • Year and Date: 2014-09-29 – 2014-10-01
• [Presentation] プラミペキソールはラット線条体へのピロカルピン投与によって誘導されるパーキンソン病様振戦を減弱させる (2014)
  • Author(s): 保田和哉，安部博史，小金丸剛，蛯原功介，池田哲也，松尾寿栄，倉増亜紀，船橋英樹，武田龍一郎，有森和彦，石田康
  • Organizer: 第36回日本生物学的精神医学会・第57回日本神経化学会大会
  • Place of Presentation: 奈良県文化会館・奈良県新公会堂（奈良県奈良市）
  • Year and Date: 2014-09-29 – 2014-10-01
• [Presentation] 抑肝散の認知症に対する臨床効果 (2014)
  • Author(s): 石田康，林要人，岡原一徳
  • Organizer: 第36回日本生物学的精神医学会・第57回日本神経化学会大会
  • Place of Presentation: 奈良県文化会館・奈良県新公会堂（奈良県奈良市）
  • Year and Date: 2014-09-29 – 2014-10-01
• [Presentation] MHC クラス 1 のコカインの自己投与への関与 (2014)
  • Author(s): 村上元,Hongrui Meng,枝村光浩,古川智範,福田敦夫,岩下寿秀，石田康，中原大一郎
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] Effects of dopamine agonist, cabergoline on the tremulous jaw movements produced by the acetylcholinesterase inhibitor, tacrine, and their related patterns of c-Fos expression in the striatum (2013)
  • Author(s): Koganemaru G, Abe H, Kuramashi A, Ebihara K, Matsuo H, Funahashi H, Yasuda K, Ikeda T, Takeda R, Nishimori T, Ishida Y
  • Organizer: 11th World Congress of Biological Psychiatry
  • Place of Presentation: Kyoto
• [Presentation] 巻き貝の神経ペプチドがヒトの苦痛を救うかもしれない？ (2013)
  • Author(s): 池田哲也，井川加織，中村武宏，武田龍一郎，石田康，西森利數
  • Organizer: 第38回日本比較内分泌学会大会
  • Place of Presentation: 宮崎市
• [Presentation] Long-lasting effects of adolescent nicotine exposure on cognitive function and neuronal development in mice (2013)
  • Author(s): Suenaga T, Ogawa Y, Meng H, Ishida Y, Nakahara D
  • Organizer: 43th Annual Meeting Society for Neuroscience
  • Place of Presentation: San Diego, USA
• [Presentation] Effects of dopamine receptor agonist, cabergoline or rotigotine on tremulous jaw movements induced by acetylcholinesterase inhibitor, tacrine (2013)
  • Author(s): Koganemaru G, Abe H, Kuramashi A, Ebihara K, Matsuo H, Funahashi H, Yasuda K, Ikeda T, Takeda R, Nishimori T, Ishida Y
  • Organizer: 43th Annual Meeting Society for Neuroscience
  • Place of Presentation: San Diego, USA
• [Presentation] Effect of pretreatment with [Leu11]-hemokinin-1 on scratching induced by histamine or serotonin (2012)
  • Author(s): Funahashi H, Naono-Nakayama R, Morishita M, Abe H, Matsuo H, Kuramashi A, Koganemaru G, Ikeda T, Ishida Y, Nishimori T
  • Organizer: 42th Annual Meeting Society for Neuroscience
  • Place of Presentation: New Orleans
• [Presentation] Effect of perospirone on methamphetamine-induced disruption of latent inhibition in rats (2012)
  • Author(s): Matsuo H, Abe H, Okada N, Kuramashi A, Koganemaru G, Funahashi H, Ikeda T, Takeda R, Ebihara K, Nishimori T, Ishida Y
  • Organizer: 42th Annual Meeting Society for Neuroscience
  • Place of Presentation: New Orleans
• [Presentation] Methamphetamine誘発性潜在抑制障害に対するblonanserinの改善効果
  • Author(s): 倉増亜紀，安部博史，小金丸剛，松尾寿栄，船橋英樹，保田和哉，武田龍一郎，蛯原功介，池田哲也，西森利数，石田康
  • Organizer: DSフォーラム2014
  • Place of Presentation: 神戸市