Generation of iPS cells from dental pulp of autism patients
| Project/Area Number |
24591693
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | Institute for Developmental Research, Aichi Human Service Center |
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Principal Investigator |
Nakayama Atsuo 愛知県心身障害者コロニー発達障害研究所, 発生障害学部, 部長 (50227964)
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| Co-Investigator(Kenkyū-buntansha) |
FUKADA Masahide 愛知県心身障害者コロニー発達障害研究所, 発生障害学部, 研究員 (80414019)
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| Research Collaborator |
IIO Akio
MATSUKI Tohru
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 自閉症 / iPS細胞 / 神経細胞 / 研究リソース / 歯髄細胞 / 神経細胞分化 / ニューロリギン４X / 発達障害 / 歯髄幹細胞 / 自閉症スペクトラム障害 |
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| Outline of Final Research Achievements |
In order to establish research materials useful for revealing neuronal abnormalities in autistic patient, we have introduced re-programing agents into dental pulp stem cells obtained from 10 autistic patients. During the period, we could established two induced pluripotent stem (iPS) cell clones derived from autistic patients. These iPS cell clones can differentiate into the neuronal lineage expressing several markers for neuronal precursor cells. At this differentiated stage, however, we could not find clear abnormalities in morphology and proliferative potential compared with neuronal precursor cells differentiated from control iPS cells. In spite of the results, we emphasize that these iPS clones derived from autistic patients are useful materials to reveal detailed neuronal abnormalities of autism in future in vitro studies.
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Report
(5 results)
Research Products
(11 results)
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[Journal Article] Characterisation of novel RUNX2 mutation with alanine tract expansion from Japanese cleidocranial dysplasia patient2016
Author(s)
Shibata A., Machida J., Yamaguchi S., Kimura M., Tatematsu T., Miyachi H, Matsushita M., Kitoho H., Ishiguro N., Nakayama A., Higashi Y., Shimozato K., Tokita Y
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Journal Title
Mutagenesis
Volume: 31
Pages: 61-67
Related Report
Peer Reviewed / Open Access
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[Journal Article] An aberrant splice acceptor site due to a novel intronic nucleotide substitution in MSX1 gene is the cause of congenital tooth agenesis in a Japanese family2015
Author(s)
Tatematsu T., Kimura M., Nakashima M., Machida J., Yamaguchi S., Shibata A., Goto H., Nakayama A., Higashi Y., Miyachi H., Shimozato K., Matsumoto N., Tokita Y
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Journal Title
PLoS One
Volume: 10
Pages: 1-6
Related Report
Peer Reviewed / Open Access
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[Journal Article] Novel nonsense mutation in MSX1 in familial nonsyndromic oligodentia: subcellular loalization and role of homeodomain/MH4.2014
Author(s)
Kimura M., Machida J., Yamaguchi S., Shibata A., Tatematsu T., Miyachi H., Jezewski PA., Nakayama A., Higashi Y., Shimozato K., Tokita Y.
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Journal Title
Eur J Oral Sci
Volume: 122(1)
Issue: 1
Pages: 15-20
DOI
Related Report
Peer Reviewed
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