Detection of activated platelet on thrombus using fucoidan conjugated with USPIO

• Project/Area Number: 24591822
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: Juntendo University
• Principal Investigator: SUZUKI Michimasa 順天堂大学, 医学部, 准教授 (20534811)
• Co-Investigator(Kenkyū-buntansha): KYOGOKU Shinsuke 順天堂大学, 放射線診断学講座, 准教授 (60205028) ; KIMIZUKA Takao 順天堂大学, 放射線診断学講座, 准教授 (70348952)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 分子イメージング / 血栓 / 活性化血小板 / 国際情報交換 / 国際研究者交流フランス / 国際研究者交流 フランス INSERM

Outline of Final Research Achievements

There is no established method to see the activated platelet on MRI scanner. In this decade, several researcher works on this issue, and developed several contrast agents. However, its safety and feasibility is still questionable. We developed a novel contrast material using USPIO conjugated with fucoidan, that is mucopolysacchrides extracted from sea weeds. In a present study, we made a activated thrombus model with rats. In a acute cerebral infarction model, we could see the enhancement of the region on MRI. Consequently, affinity of fucoidan and P-selectin was examined gently on aortic abdominal aneurysm model. Using an immunohistochemical stain toward P-selectin and an electron microscopy, distribution of USPIO and activated platelets were confirmed.

Research Products

• [Journal Article] Ultrasmall superparamagnetic iron oxide nanoparticles coated with fucoidan for molecular MRI of intraluminal thrombus. (2015)
  • Author(s): Suzuki M, Bachelet-Violette L, Rouzet F, Beilvert A, Autret G, Maire M, Menager C, Louedec L, Choqueux C, Saboural P, Haddad O, Chauvierre C, Chaubet F, Michel JB, Serfaty JM, Letourneur D
  • Journal Title: Nanomedicine Volume: 10 Issue: 1 Pages: 73-87
  • DOI: 10.2217/nnm.14.51
  • Peer Reviewed / Open Access / Acknowledgement Compliant