The study of anti-tumor with anti-metastasis under combination of 4-methylumbelliferone on X-irradiation against cancer cell

• Project/Area Number: 24591831
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: Hirosaki University
• Principal Investigator: Hosokawa Yoichiro 弘前大学, 保健学研究科, 教授 (70173599)
• Co-Investigator(Renkei-kenkyūsha): MONZEN Satoru 弘前大学, 大学院保健学研究科, 助教 (20514136) ; TERASHIMA Shingo 弘前大学, 大学院保健学研究科, 助教 (00583733) ; YOSHINO Hironori 弘前大学, 大学院保健学研究科, 助教 (10583734)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: ４－メチルウンベリフェロン / 放射線治療 / 転移 / サイトカイン / 放射線 / 抗癌作用 / ４-メチルウンベリフェロン / 浸潤 / 4-メチルウンベリフェロン

Outline of Final Research Achievements

Radiotherapy is generally effective against cancer, however distant metastasis cannot be controlled by radiotherapy alone. Our experiments are studied to develop new radiotherapy combined with 4-Methylumbelliferone (MU) in order to prevent metastasis of cancer cells. On this study, the treatment of radiation combined with MU showed anti-cancer and anti-metastatic effects compared with radiation alone. MU is hoped to apply in a clinical setting as anti-metastatic drug on radiotherapy.

Research Products

• [Journal Article] Effects of X-ray irradiation in combination with ascorbic acid on tumor control. (2015)
  • Author(s): Hosokawa Y, Monzen S, Yoshino H, Terashima S, Nakano M, Toshima K, Saga R, Kashiwakura I
  • Journal Title: Mol Med Rep. Volume: 12 Pages: 5449-5454
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Study of OH radicals scavenging initiated by X-ray radiation in human plasma (2015)
  • Author(s): Yoichiro Hosokawa , Tomoaki Sano
  • Journal Title: Radiation Protection Dosimetry Volume: 165 Issue: 1-3 Pages: 1-5
  • DOI: 10.1093/rpd/ncv272
  • Peer Reviewed
• [Journal Article] Analysis Using an NaI(Tl) Detector for Radioactive Materials When Dismantling a Linear Accelerator (2015)
  • Author(s): Shingo Terashima, Naoki Kimura, Morio Seino, Mamoru Kubota
  • Journal Title: Emergency Radiation Medicine Volume: 4 Pages: 47-52
  • NAID: 40020370770
  • Peer Reviewed / Open Access
• [Journal Article] Method for Reducing Skin Dose under IVR Pulsed Fluoroscopic X-ray Irradiation (2015)
  • Author(s): Kohsei Kudo, Minoru Osanai, Junichi Hirota, Sachio Sato, Satoshi Naraki, Katumasa Suzaki, Akira Fujimori, Yoshihiro Takai and Yoichiro Hosokawa
  • Journal Title: Emergency Radiation Medicine Volume: 4 Pages: 34-39
  • Peer Reviewed / Open Access
• [Journal Article] Cytotoxic effects of hyper-radiation sensitivity by low dose multiple exposures. (2014)
  • Author(s): Yui Inoue, Shingo Terashima, Yoichiro Hosokawa
  • Journal Title: The Proceedings of the 5ｔｈ International Symposium on Radiation Emergency Medicine at Hirosaki University Volume: 4 Pages: 15-17
• [Journal Article] 4-methylumbelliferone’s addition against human fibrosarucoma cells and the effect of radiation concomitant use (2014)
  • Author(s): Ryo Saga, Yoichiro Hosokawa, Hironori Yoshino, Shingo Terashima, Toshiya Nakamura
  • Journal Title: The Proceedings of the 5ｔｈ International Symposium on Radiation Emergency Medicine at Hirosaki University Volume: 4 Pages: 36-38
• [Journal Article] Acute promyelocytic leukemia mutated to radioresistance suppressed monocyte lineage differentiation by phorbol 12-myristate 13-acetate. (2013)
  • Author(s): Monzen S, Takimura K, Kashiwakura I, Hosokawa Y.
  • Journal Title: Leuk Res. Volume: 37(9) Pages: 1162-1169
  • Peer Reviewed
• [Journal Article] Effect of ascorbic acid and X-irradiation on HL-60 human leukemia cells: the kinetics of reactive oxygen species. (2013)
  • Author(s): Terashima S, Hosokawa Y, Yoshino H, Yamaguchi M, Nakamura T.
  • Journal Title: Oncol Rep. Volume: 30(6) Issue: 6 Pages: 2653-2658
  • DOI: 10.3892/or.2013.2758
  • Peer Reviewed
• [Journal Article] Thyroid screening survey on Children after the Fukushima Daiichi Nuclear Power Plant Accident (2013)
  • Author(s): Yoichiro Hosokawa, Masahiro Hosoda, Akifumi Nakata, Masanori Kon, Mayumi Urushizaka1 and Mitsuaki A Yoshida..
  • Journal Title: REM. Volume: 2(1) Pages: 82-86
  • NAID: 40019561921
  • Peer Reviewed
• [Journal Article] Novel Acridine-Based N-Acyl-homoserine Lactone Analogs Induce Endoreduplication in the Human Oral Squamous Carcinoma Cell Line SAS. (2012)
  • Author(s): Chai H, Hazawa M, Hosokawa Y, Igarashi J, Suga H, Kashiwakura I.
  • Journal Title: Biol Pharm Bull. Volume: 35(8) Pages: 1257-1263
  • NAID: 130001872135
  • Peer Reviewed
• [Journal Article] Reconsideration of the Iwasaki-Waggener iterative perturbation method for reconstructing high-energy X-ray spectra. (2012)
  • Author(s): Iwasaki A, Kimura S, Sutoh K, Kamimura K, Sasamori M, Seino M, Komai F, Terashima S, Kubota M, Narita Y, Hosokawa Y, Miyazawa M.
  • Journal Title: Radiol Phys Technol. Volume: 5(2): Pages: 248-269
  • Peer Reviewed
• [Journal Article] Radiation protective effect of hypoxia-inducible factor-1α(HIF-1α) on humanoral squamous cell carcinoma cell lines (2012)
  • Author(s): Y. Hosokawa, K. Okumura, S. Terasima, Y. Sakakura
  • Journal Title: Radiation Protection Dosimetry Volume: Vol. 152 Issue: 1-3 Pages: 159-163
  • DOI: 10.1093/rpd/ncs215
  • Peer Reviewed
• [Presentation] ヒト前立腺癌細胞に対する4-methylumbelliferoneの放射線増感効果 (2015)
  • Author(s): 嵯峨 涼, 門前 暁, 細川 洋一郎
  • Organizer: 日本放射線影響学会第1回放射線ワークショップ-未来に繋ぐ放射線研究
  • Place of Presentation: 富山大学（富山県 富山市）
  • Year and Date: 2015-10-16
• [Presentation] 4-メチルウンベリフェロンを併用したがん放射線療法の基礎的検討 (2015)
  • Author(s): 門前 暁、細川 洋一郎、嵯峨 涼、千葉 満
  • Organizer: 第25回日本サイトメトリー学会学術集会
  • Place of Presentation: お茶の水ソラシティーカンファレンス（東京都）
  • Year and Date: 2015-07-11
• [Presentation] Effect of 4-methylumbelliferone and Irradiation Combination on Factors Related to A Variety of Invasion and Metastasis. (2015)
  • Author(s): Ryo Saga, Satoru Monzen, Hironori Yoshino, Mitsuru Chiba, Toshiya Nakamura, and Yoichiro Hosokawa
  • Organizer: ICRR2015, 15th International Congress of Radiation Research
  • Place of Presentation: 京都国際会館（京都府 京都市）
  • Year and Date: 2015-05-25
  • Int'l Joint Research
• [Presentation] Effect of 4-methylumbelliferone and irradiation combination on factors related to a variety of invasion and metastasis. (2015)
  • Author(s): R. Saga, S. Monzen, H. Yoshino, M. Chiba, T. Nakamura and Y. Hosokawa
  • Organizer: ESRAH2015, 2nd Educational Symposium on Radiation and Health by Young Scientists
  • Place of Presentation: 弘前大学（青森 弘前市）
  • Year and Date: 2015-05-23
  • Int'l Joint Research
• [Presentation] Inhibition of malignant tumor metastasis by 4-methylumbelliferone on X-irradiation. (2014)
  • Author(s): Ryo Saga, Satoru Monzen, Hironori Yoshino, Mitsuru Chiba, Toshiya Nakamura, Yoichiro Hosokawa
  • Organizer: 19th World Congress on Advances in Oncology
  • Place of Presentation: ギリシャ アテネ
  • Year and Date: 2014-10-09 – 2014-10-10
• [Presentation] Acquisition of resistance to ionizing radiation in acute promyelocytic leukemia cells occurs via modifications of several mRNA expression networks. International Journal of Molecular Medicine. (2014)
  • Author(s): Monzen S, Saga R, Chiba M, Hosokawa Y.
  • Organizer: 19th World Congress on Advances in Oncology
  • Place of Presentation: ギリシャ アテネ
  • Year and Date: 2014-10-09 – 2014-10-10
• [Presentation] Study of OH radicals scavenging initiated by X-ray radiation in human plasma (2014)
  • Author(s): Yoichiro Hosokawa , Tomoaki Sano
  • Organizer: The 9th International Symposium on the Natural Radiation Environment
  • Place of Presentation: ホテルニューキャッスル（青森県弘前市）
  • Year and Date: 2014-09-22 – 2014-09-23
• [Presentation] 4-methylumbelliferone’s addition against human fibrosarucoma cells and the effect of radiation concomitant use (2013)
  • Author(s): Ryo Saga, Yoichiro Hosokawa, Hironori Yoshino, Shingo Terashima, Toshiya Nakamura
  • Organizer: The 5ｔｈ International Symposium on Radiation Emergency Medicine at Hirosaki University
  • Place of Presentation: 青森県青森市