Radiosensitization in gastric cancer cell lines using different PI3K/AKT/mTOR inhibitors
Project/Area Number |
24591850
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Radiation science
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Research Institution | Keio University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
FUKADA Junichi 慶應義塾大学, 医学部, 講師 (50338159)
NISHIMURA Shuichi 慶應義塾大学, 医学部, 助教 (40571138)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 放射線治療 / 放射線生物学 / 放射線増感剤 / 胃癌 / PI3K/Akt/mTOR経路 |
Outline of Final Research Achievements |
Treatment of gastric cancer in advanced stages is still challenging. More effective anti-tumor radiosensitizing drug is desired to manage systemic disease. PI3K/AKT/ mTOR pathway is one of the important signal cascades which are activated by various growth factor receptors. We selected three PI3K/AKT/ mTOR pathway inhibitors. Treatment effectiveness is assessed by irradiation, drug exposure alone and combination of irradiation and drug in human gastric cell lines. Cell survival was evaluated with colony formation. Apoptosis was evaluated with flow cytometric analyses using AnnexinV-FITC/PI stain. Cell cycle distribution was evaluated using flow cytometric analyses. Concurrent exposure showed larger cytotoxic effect and higher apoptotic rate than the other exposure. The G2/G1 ratio was exclusively increased by concurrent exposure with irradiation and NVP-BEZ235 after 48-hours. NVP-BEZ235 seems to be a promising radiosensitizing drug in terms of arresting cell cycle in G2/M phase.
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Report
(4 results)
Research Products
(4 results)
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[Presentation] Symptomatic pericardial effusion after chemoradiation therapy for esophageal cancer: A historical comparison.2013
Author(s)
Fukada J, Shigematsu N, Takeuchi H, Shiraishi Y, Hanada T, Ohashi T, Kota R, Kumabe A, Sutani S, Kitagawa Y.
Organizer
55th Annual Meeting of American Society for Therapeutic Radiology and Oncology
Place of Presentation
Atlanta, GA, USA
Related Report
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[Presentation] Inter- and intrafraction gastric motion study –Impact of breathing and stomach filling2012
Author(s)
J. Fukada, N. Shigematsu, T. Hanada, T. Ohashi, T. Tanaka, Y. Shiraishi, H. Takeuchi, Y. Kitagawa
Organizer
American society for radiation oncology 54th annual meeting
Place of Presentation
Boston convention and exhibition center, Boston, MA, USA
Related Report