Possibilities of mesothelioma treatment through miRNA expressed by high LET heavy ion beams

• Project/Area Number: 24591859
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: National Institute of Radiological Sciences
• Principal Investigator: LIU CUIHUA 独立行政法人放射線医学総合研究所, 放射線防護研究センター, 主任研究員 (00512427)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 悪性中皮腫 / 高LET重粒子線 / miRNAのプロファイリング / miRNAの発現 / miRNA-mimic / miRNA-inhibitor / 細胞生存率 / 細胞増殖能 / 重粒子線 / miRNA profile / miRNA mimic / miRNA inhibitor / 細胞増殖 / テーラーメード / 炭素線 / 高LET / miRNA / miRNAプロファィリング / Ｘ線 / 細胞致死メカニズム / miRNAプロファイリング

Outline of Final Research Achievements

Malignant mesothelioma cells were irradiated with X-rays or high-LET carbon ion beams. Total RNA includes miRNA was extracted and miRNA profiling was analyzed. The results showed the miRNA profiling for mesothelioma cells irradiated by high-LET carbon ion and X-rays was different. Different mesothelioma cell lines have different miRNA profiling. Comparing X-rays, the miR-144-3p in MESO-1 cells was expressed higher irradiated by high-LET carbon ions. Using the miR-144-3p mimic that was transfected into MESO-1 cells, the propagation of cells was inhibited by the miR-144-3P significantly. Also, the miR-718 mimic can inhibit growing of MESO-4 cells. Those results showed using special miRNA, suggested that it might be very important for the tailor-made medical care of mesothelioma patients.

Research Products

• [Journal Article] The complexity of DNA double strand break is a crucial factor for activating ATR signaling pathway for G2/M checkpoint regulation regardless of ATM function (2015)
  • Author(s): Xue L, Furusawa Y, Okayasu R, Miura M, Cui X, Liu C, Hirayama R, Matsumoto Y, Yajima H, Yu D.
  • Journal Title: DNA Repair (Amst). Volume: 25 Pages: 72-83
  • Peer Reviewed / Open Access
• [Journal Article] Genetic changes in progeny of bystander human fibroblasts after microbeam irradiation with X-rays, protons or carbon ions: the relevance to cancer risk (2015)
  • Author(s): Autsavapromporn N, Plante I, Liu C, Konishi T, Usami N, Funayama T, Azzam EI, Murakami T, Suzuki M.
  • Journal Title: Int J Radiat Biol Volume: 91（１） Issue: 1 Pages: 62-70
  • DOI: 10.3109/09553002.2014.950715
  • Peer Reviewed / Open Access
• [Journal Article] Effects of shielding on the induction of 53BP1 foci and micronuclei after Fe ion exposures (2014)
  • Author(s): Hu W, Pei H, Li H, Ding N, He J, Wang J, Furusawa Y, Hirayama R, Matsumoto Y, Liu C, Li Y, Kawata T, Zhou G.
  • Journal Title: J Radiat Res Volume: 55(1) Issue: 1 Pages: 10-16
  • DOI: 10.1093/jrr/rrt078
  • Peer Reviewed / Open Access
• [Journal Article] Comparison of the repair of potentially lethal damage after low- and high-LET radiation exposure, assessed from the kinetics and fidelity of chromosome rejoining in normal human fibroblasts. (2013)
  • Author(s): Liu C, Kawata T, Zhou G, et al
  • Journal Title: J Radiat Res Volume: 54 Issue: 6 Pages: 989-997
  • DOI: 10.1093/jrr/rrt031
  • Peer Reviewed
• [Journal Article] Chromosome aberrations in normal human fibroblasts analyzed in G0/G1 and G2/M phases after exposure in G0 to radiation with different linear energy transfer (LET). (2013)
  • Author(s): Liu C, Kawata T, et al
  • Journal Title: Mutat Res Volume: 756 Issue: 1-2 Pages: 101-107
  • DOI: 10.1016/j.mrgentox.2013.05.005
  • Peer Reviewed
• [Journal Article] Participation of Gap Junction Communication in Potentially Lethal Damage Repair and DNA Damage in Human Fibroblasts Exposed to Low-or High-LET Radiation. (2013)
  • Author(s): Narongchai Autsavapromporn, et al.
  • Journal Title: Mutation Research, Genetic Toxicology and Environmental Mutagenesis Volume: 756 Issue: 1-2 Pages: 78-85
  • DOI: 10.1016/j.mrgentox.2013.07.001
  • Peer Reviewed
• [Journal Article] Simulated studies on the biological effects of space radiationSimulated studies on the biological effects of space on quiescent human fibroblasts (2013)
  • Author(s): Nan Ding , Hailong Pei , Jinpeng He , Yoshiya Furusawa , Ryoichi Hirayama , Cuihua Liu , Yoshitaka Matsumoto , He Li , Wentao Hu , Yinghui Li , Jufang Wang
  • Journal Title: Advances in Space Research Volume: 52 Issue: 7 Pages: 1314-1319
  • DOI: 10.1016/j.asr.2013.06.030
  • Peer Reviewed
• [Journal Article] Modeling the biological response of normal human cells, including repair processes, to fractionated carbon beam irradiation (2013)
  • Author(s): Wada M, Suzuki M, Liu C, Kaneko Y, Fukuda S, Ando K, Matsufuji N.
  • Journal Title: Journal of Radiation Research Volume: accept
  • Peer Reviewed
• [Presentation] High linear energy transfer sensitization of MPM cells by mitotic catastrophe pathway (2014)
  • Author(s): 劉 翠華、Narongchai Autsavapromporn
  • Organizer: 放射線影響学会第57回大会
  • Place of Presentation: かごしま県民交流センター
  • Year and Date: 2014-10-01 – 2014-10-03
• [Presentation] DNA二本鎖切断の修復過程におけるDNA末端の削り込みと細胞応答 (2014)
  • Author(s): 矢島浩彦 , 劉翠華 , 中島 菜花子
  • Organizer: 放射線影響学会第57回大会
  • Place of Presentation: かごしま県民交流センター
  • Year and Date: 2014-10-01 – 2014-10-03
• [Presentation] Dose, LET and Ion Species Dependence for PLDR in Normal Human Embryo Fibroblasts (2013)
  • Author(s): 劉 翠華 , 鶴岡 千鶴 , アッサワプロンポーン ナロンチャイ , 金子 由美子 , 村上 健
  • Organizer: 日本放射線影響学会第56回大会
  • Place of Presentation: ホテルクラウンパレス青森
• [Presentation] Intercellular Communication Between Carbon-Irradiated Cancer Cells and Non-Irradiated Bystander Fibroblasts: The Impact of Fractionated Irradiation (2013)
  • Author(s): Narongchai Autsavapromporn , Masao Suzuki , Cuihua Liu , Takeshi Murakami
  • Organizer: 52nd Annual Conference of the Particle Therapy Co-Operative Group
  • Place of Presentation: The Congress Center Essen
• [Presentation] 炭素イオンビームにおける中皮腫細胞致死メカニズムの解析 (2012)
  • Author(s): 劉 翠華, 鈴木 雅雄, アッサワプロンポーン ナロンチャイ, Ｃｈｅｗ Ｍｉｎｇ Ｔｓｕｅｙ, 金子 由美子, 村上 健
  • Organizer: 日本放射線影響学会第５５回大会
  • Place of Presentation: 仙台
• [Presentation] 炭素イオンにおける中皮腫細胞致死および細胞致死メカニズムについて (2012)
  • Author(s): 劉 翠華、 鈴木 雅雄
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] Comparison on the repair of potentially lethal damage between exposure to low- and high-LET radiations from Kinetics and fidelity of chromosome rejoining in normal human fibroblasts (2012)
  • Author(s): Cuihua Liu, Tetsuya Kawata, Naoyuki Shigematsu, Kerry George, Francesca A Cucinotta
  • Organizer: Radiation Research Society 58th Annual Meeting
  • Place of Presentation: アメリカ プエルトリコ
• [Presentation] Comparison of chromosome aberrations in G0/G1 and G2/M cells in normal human fibroblasts irradiated at G0 phase with various LETs (2012)
  • Author(s): Cuihua Liu, Tetsuya Kawata, Ryuichi Okayasu, Guangming Zhou, Yoshiya Furusawa, et.al
  • Organizer: The 10th International Symposium on chromosomal Aberrations
  • Place of Presentation: Italy,Amalfi
• [Remarks] 放射線医学総合研究所 学術機関リポジトリ
  • URL: http://repo.nirs.go.jp/?action=pages_view_main&active_action=repository_view_main_item_snippet&all=liu%20cui%20hua&count=50&order=10&pn=1&st=1&page_id=13&block_id=8
• [Remarks] 放医研 業務実績登録システム
  • URL: http://dpop02.nirs.go.jp/dpop/search.php?mode=g&s=%E5%8A%89%E7%BF%A0%E8%8F%AF&sy=&ey=&fy=