| Project/Area Number |
24591863
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
General surgery
|
|---|
| Research Institution | University of Tsukuba |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OHKOHCHI Nobuhiro 筑波大学, 医学医療系, 教授 (40213673)
TADANO Sosuke 筑波大学, 医学医療系, 講師 (70570688)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Keywords | 外科総論 / 創傷治癒 / 消化管吻合 / PRP / 線維芽細胞 / Collagen / 血小板 / 成長因子 |
|---|
| Outline of Final Research Achievements |
The aim of the present study was to investigate how human platelet rich plasma (PRP) enhances intestinal anastigmatic healing. In the in vitro setting, human PRP exerted the cell growth-promoting effect on human colonic fibroblasts, however its effect was reduced in high concentration (so-called “ceiling effect”). In terms of collagen production, human PRP increased the collagen type I concentration in the co-culture supernatant, which might be induced by promoting proliferation of human colonic fibroblasts but not enhancement of each cell’s ability to produce collagen. In other words, human PRP could not accelerate collagen production, and hardly stimulated collagen production. The main effect of human PRP on human colonic fibroblasts appeared to be the cell growth-promoting action, thereby allowing for increased production of collagen.
|
