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Development of new technique using platelet for prevention of anastomotic leakage

Research Project

Project/Area Number 24591863
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General surgery
Research InstitutionUniversity of Tsukuba

Principal Investigator

TERASHIMA Hideo  筑波大学, 医学医療系, 教授 (10361338)

Co-Investigator(Kenkyū-buntansha) OHKOHCHI Nobuhiro  筑波大学, 医学医療系, 教授 (40213673)
TADANO Sosuke  筑波大学, 医学医療系, 講師 (70570688)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords外科総論 / 創傷治癒 / 消化管吻合 / PRP / 線維芽細胞 / Collagen / 血小板 / 成長因子
Outline of Final Research Achievements

The aim of the present study was to investigate how human platelet rich plasma (PRP) enhances intestinal anastigmatic healing. In the in vitro setting, human PRP exerted the cell growth-promoting effect on human colonic fibroblasts, however its effect was reduced in high concentration (so-called “ceiling effect”). In terms of collagen production, human PRP increased the collagen type I concentration in the co-culture supernatant, which might be induced by promoting proliferation of human colonic fibroblasts but not enhancement of each cell’s ability to produce collagen. In other words, human PRP could not accelerate collagen production, and hardly stimulated collagen production. The main effect of human PRP on human colonic fibroblasts appeared to be the cell growth-promoting action, thereby allowing for increased production of collagen.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (2 results)

All 2014

All Presentation (2 results)

  • [Presentation] 血小板は消化管吻合部における創傷治癒を促進する:ヒト腸管由来線維芽細胞を用いた検討2014

    • Author(s)
      松村英樹,寺島秀夫,只野惣介,村田聡一郎,大河内信弘
    • Organizer
      第114回日本外科学会定期学術集会
    • Place of Presentation
      国立京都国際会館(京都府京都市)
    • Year and Date
      2014-04-03 – 2014-04-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] 血小板は消化管吻合部における創傷治癒を促進する. ヒト腸管由来線維芽細胞を用いた検討2014

    • Author(s)
      松村英樹
    • Organizer
      第114回日本外科学会定期学術集会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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