The effect of focal mechanical stress on the localization of TRPV2 was investigated in the fibrosarcoma cells.

• Project/Area Number: 24591865
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: Gunma University
• Principal Investigator: nagasawa masahiro 群馬大学, 生体調節研究所, 助教 (50343083)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: TRPV2 / トランスロケーション / 機械受容カルシウムチャネル / 伸展刺激 / カルシウム / TRPチャネル / TRPV2チャネル / 機械刺激 / カルシウムチャネル

Outline of Final Research Achievements

The effect of focal mechanical stress on the localization of TRPV2 was investigated in HT1080 cells. Mechanical stress was applied by adding negative pressure using a glass pipette.When focal mechanical stress was applied, subplasma membrane Ca2+ concentration ([Ca2+]s) was increased beneath the pipette, which propagated throughout the cell.The increase in [Ca2+]s was blocked by knocking down TRPV2.Elevation of [Ca2+]s was not observed by removal of extracellular Ca2+, by an addition of a phosphatidylinositol 3-kinase inhibitor.In cells expressing GFP-TRPV2 and RFP-Akt,administration of focal mechanical stress induced accumulation of GFP-TRPV2 beneath the pipette.RFP-Akt was also accumulated to the same site.When GFP-TRPV1,3,V4,V5,V6 was transfected ectopically in HT1080 cells, only GFP-TRPV4 was accumulated beneath the pipette in response to the focal mechanical stress.These results indicate that TRPV2 translocates to the site receiving a focal mechanical stress and increases [Ca2+]s.

Research Products

• [Journal Article] Translocation of TRPV2 channel induced by focal administration of mechanical stress. (2015)
  • Author(s): Nagasawa M, Kojima I.
  • Journal Title: Physiol Rep Volume: 3
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Glucose Evokes Rapid Ca2+ and Cyclic AMP Signals by Activating the Cell-Surface Glucose-Sensing Receptor in Pancreatic β-Cells. (2015)
  • Author(s): Nakagawa Y, Nagasawa M, Medina J, Kojima I.
  • Journal Title: PLoS One. Volume: 12
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Return of the glucoreceptor: Glucose activates the glucose-sensing receptor T1R3 and facilitates metabolism in pancreatic β-cells. (2015)
  • Author(s): Kojima I, Nakagawa Y, Ohtsu Y, Hamano K, Medina J, Nagasawa M
  • Journal Title: J Diabetes Investig. Volume: 3 Pages: 256-63
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Glucose-Sensing Receptor T1R3: A New Signaling Receptor Activated by Glucose in Pancreatic β-Cells. (2015)
  • Author(s): Kojima I, Nakagawa Y, Hamano K, Medina J, Li L, Nagasawa M.
  • Journal Title: Biol Pharm Bull. Volume: 5 Pages: 674-9
  • NAID: 130005068103
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Translocation of TRPV2 channel induced by focal administration of mechanical stress. (2015)
  • Author(s): Nagasawa M, Kojima I.
  • Journal Title: Physiol Rep. Volume: 3
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 膵β細胞に発現するグルコース感知受容体 (2015)
  • Author(s): 小島至、ヨハン・メディナ、中川祐子、濱野邦久、長澤雅裕
  • Organizer: 生理学研究所 研究会「生体シグナルダイナミクス」
  • Place of Presentation: 生理研研究所(愛知県岡崎市）
  • Year and Date: 2015-09-03
• [Presentation] TRPV2 (2014)
  • Author(s): 長澤 雅裕、小島 至
  • Organizer: 第１０回TRP研究会
  • Place of Presentation: 岡崎カンファレンスセンター
  • Year and Date: 2014-06-05 – 2014-06-06
• [Presentation] 機械受容チャネルTRPV2の制御機構 (2013)
  • Author(s): 長澤 雅裕 小島 至
  • Organizer: 第9回TRPチャネル研究会
  • Place of Presentation: 岡崎カンファレンスセンター
• [Presentation] がん細胞に発現するTRPV2:機械刺激に対する応答 (2012)
  • Author(s): 長澤 雅裕 小島 至
  • Organizer: 第8回TRPチャネル研究会
  • Place of Presentation: 岡崎カンファレンスセンター
• [Book] Mammalian Transient Receptor Potential (TRP) Cation Channels Volume1 PartⅡ The TRPV Subfamily TRPV2 (2015)
  • Author(s): Kojima I. and Nagasawa M
  • Publisher: Springer