Impact of nitric oxide with oxygen gas persufflation on marginal graft in liver transplantation

• Project/Area Number: 24591877
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: Kyoto University
• Principal Investigator: YAGI Shintaro 京都大学, 医学(系)研究科(研究院), 助教 (60447969)
• Co-Investigator(Kenkyū-buntansha): OGAWA Kohei 京都大学, 医学(系)研究科(研究院), 助教 (10359789) ; OGURA Yasuhiro 名古屋大学, 医学部附属病院, 准教授 (20335251) ; KAIDO Toshimi 京都大学, 医学(系)研究科(研究院), 准教授 (80314194) ; UEMOTO Shinji 京都大学, 医学(系)研究科(研究院), 教授 (40252449)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 肝移植 / 一酸化窒素 / 過少グラフト / 境界グラフト / 門脈圧 / 心停止ドナー

Outline of Final Research Achievements

We established small-for-size graft liver transplantation model in pig, which is 30% partial liver graft volume using right-lateral lobe. We investigated the effect of nitric oxide (NO) gas during liver preservation on small-for-size livers after transplantation. Gaseous oxygen with NO (40 ppm) was insufflated into the livers through the suprahepatic vena cava during cold storage (VSNOP group). Livers in Control group was preserved by simple cold storage.
One recipients in VSNOP group survived for 7 days, and the survival rates in VSNOP group tended to be better compared with control group. Pressure gradient between portal vein and inferior vena cava of livers preserved by VSNOP tended to be lower compared with the control livers just behind portal reperfusion.

Research Products

• [Journal Article] Ultraviolet-induced alloantigen-specific immunosuppression in transplant immunity. (2015)
  • Author(s): Hori T, Kuribayashi K, Saito K, Wang L, Torii M, Uemoto S, Iida T, Yagi S, Kato T.
  • Journal Title: World J Transplant Volume: 5 Issue: 1 Pages: 11-18
  • DOI: 10.5500/wjt.v5.i1.11
  • Peer Reviewed / Open Access
• [Journal Article] Significance of (99m)Tc-GSA liver scintigraphy in liver surgery and transplantation. (2015)
  • Author(s): Iida T, Yagi S, Hori T, Uemoto S.
  • Journal Title: Ann Transl Med. Volume: 3 Pages: 16-16
  • Peer Reviewed / Open Access
• [Journal Article] Long-term survival after resection of pancreatic cancer: a single-center retrospective analysis. (2015)
  • Author(s): Yamamoto T, Yagi S, Kinoshita H, Sakamoto Y, Okada K, Uryuhara K, Morimoto T, Kaihara S, Hosotani R.
  • Journal Title: World J Gastroenterol. Volume: 7 Issue: 11 Pages: 262-8
  • DOI: 10.4240/wjgs.v5.i11.309
  • Peer Reviewed / Open Access
• [Journal Article] Indication for neoadjuvant chemotherapy in patients with colorectal liver metastases based on a nomogram that predicts disease-free survival. (2014)
  • Author(s): Okuno M, Hatano E, Seo S, Taura K, Yasuchika K, Nakajima A, Yazawa T, Furuyama H, Kawamoto H, Yagi S, Nishitai R, Fujikawa T, Arimoto A, Zaima M, Yoshimura T, Terajima H, Kaihara S, Manaka D, Tanaka A, Uemoto S.
  • Journal Title: J Hepatobiliary Pancreat Sci. Volume: 21 Issue: 12 Pages: 881-888
  • DOI: 10.1002/jhbp.149
  • Peer Reviewed
• [Journal Article] Donor morbidity in right and left hemiliver living donor liver transplantation: the impact of graft selection and surgical innovation on donor safety. (2014)
  • Author(s): Iwasaki J, Iida T, Mizumoto M, Uemura T, Yagi S, Hori T, Ogawa K, Fujimoto Y, Mori A, Kaido T, Uemoto S.
  • Journal Title: Transpl Int. Volume: 27 Issue: 11 Pages: 1205-13
  • DOI: 10.1111/tri.12414
  • Peer Reviewed / Open Access
• [Journal Article] Hepatic arterial complications in adult living donor liver transplant recipients: a single-center experience of 673 cases. (2014)
  • Author(s): Iida T, Kaido T, Yagi S, Hori T, Uchida Y, Jobara K, Tanaka H, Sakamoto S, Kasahara M, Ogawa K, Ogura Y, Mori A, Uemoto S.
  • Journal Title: Clin Transplant. Volume: 28 Issue: 9 Pages: 1025-30
  • DOI: 10.1111/ctr.12412
  • Peer Reviewed / Open Access
• [Journal Article] Impact of oxygen free radicals in rat partial liver transplantation. (2014)
  • Author(s): Srinivasan PK, Yagi S, Nagai K, Afify M, Hata K, Uemoto S, Tolba RH.
  • Journal Title: J Surg Res. Volume: 191 Issue: 2 Pages: 469-75
  • DOI: 10.1016/j.jss.2014.05.002
  • Peer Reviewed
• [Journal Article] Hydroxyectoine ameliorates preservation injury in deceased after cardiac death donors in experimental liver grafts. (2014)
  • Author(s): Kadaba Srinivasan P, Fet N, Bleilevens C, Afify M, Doorschodt B, Yagi S, van Echten-Deckert G, Tolba RH.
  • Journal Title: Ann Transplant. Volume: 14 Pages: 165-73
  • DOI: 10.12659/aot.889958
  • Peer Reviewed / Open Access
• [Journal Article] Graft reconditioning with nitric oxide gas in rat liver transplantation from cardiac death donors. (2014)
  • Author(s): Kageyama S, Yagi S, Tanaka H, Saito S, Nagai K, Hata K, Fujimoto Y, Ogura Y, Tolba R, Shinji U.
  • Journal Title: Transplantation. Volume: 27 Issue: 6 Pages: 618-25
  • DOI: 10.1097/tp.0000000000000025
  • NAID: 120005613678
  • Peer Reviewed
• [Journal Article] Case of primary hepatic gastrinoma: Diagnostic usefulness of the selective arterial calcium injection test. (2014)
  • Author(s): Ogawa S, Wada M, Fukushima M, Shimeno N, Inoue S, Chung H, Fujita M, Suginoshita Y, Okada A, Inokuma T, Yagi S, Ito K, Imai Y.
  • Journal Title: Hepatol Res. Volume: 22 Issue: 7 Pages: 1-4
  • DOI: 10.1111/hepr.12404
  • Peer Reviewed / Open Access
• [Journal Article] How do transplant surgeons accomplish optimal portal venous flow during living-donor liver transplantation? Noninvasive measurement of indocyanine green elimination rate. (2014)
  • Author(s): Hori T, Ogura Y, Yagi S, Iida T, Taniguchi K, El Moghazy WM, Hedaya MS, Segawa H, Ogawa K, Kogure T, Uemoto S.
  • Journal Title: Surg Innov. Volume: 21 Issue: 1 Pages: 43-51
  • DOI: 10.1177/1553350613487803
  • Peer Reviewed
• [Journal Article] Impact of preoperative uncontrollable hepatic hydrothorax and massive ascites in adult liver transplantation. (2014)
  • Author(s): Endo K, Iida T, Yagi S, Yoshizawa A, Fujimoto Y, Ogawa K, Ogura Y, Mori A, Kaido T, Uemoto S.
  • Journal Title: Surg Today. Volume: 44 Issue: 12 Pages: 2293-9
  • DOI: 10.1007/s00595-014-0839-y
  • NAID: 120005682547
  • Peer Reviewed / Open Access
• [Journal Article] How to successfully resect 70 % of the liver in pigs to model an extended hepatectomy with an insufficient remnant or liver transplantation with a small-for-size graft. (2014)
  • Author(s): Hori T, Yagi S, Okamua Y, Iida T, Ogawa K, Tanaka H, Kageyama S, Hirao H, Hata T, Kirino I, Nagai K, Kubora T, Jobara K, Endo K, Uemoto S.
  • Journal Title: Surg Today. Volume: 44 Issue: 11 Pages: 2201-7
  • DOI: 10.1007/s00595-014-0862-z
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Predictive factors for reintubation following noninvasive ventilation in patients with respiratory complications after living donor liver transplantation. (2013)
  • Author(s): Chihara Y, Egawa H, Oga T, Tsuboi T, Handa T, Yagi S, Iida T, Yoshizawa A, Yamamoto K, Mishima M, Tanaka K, Uemoto S, Chin K.
  • Journal Title: PLoS One. Volume: 8 Issue: 12 Pages: e81417-e81417
  • DOI: 10.1371/journal.pone.0081417
  • NAID: 120005372923
  • Peer Reviewed
• [Journal Article] Impact of sarcopenia on survival in patients undergoing living donor liver transplantation. (2013)
  • Author(s): Kaido T, Ogawa K, Fujimoto Y, Ogura Y, Hata K, Ito T, Tomiyama K, Yagi S, Mori A, Uemoto S.
  • Journal Title: Am J Transplant. Volume: 13 Issue: 6 Pages: 1549-1556
  • DOI: 10.1111/ajt.12221
  • Peer Reviewed
• [Journal Article] Surgical procedures for a rat model of partial orthotopic liver transplantation with hepatic arterial reconstruction. (2013)
  • Author(s): Nagai K, Yagi S, Uemoto S, Tolba RH.
  • Journal Title: J Vis Exp. Volume: 7 Issue: 73
  • DOI: 10.3791/4376
  • Peer Reviewed
• [Journal Article] A novel organ preservation for small partial liver transplantations in rats: venous systemic oxygen persufflation with nitric oxide gas. (2013)
  • Author(s): Yagi S, et al.
  • Journal Title: Am J Transplant. Volume: 13(1) Issue: 1 Pages: 222-228
  • DOI: 10.1111/j.1600-6143.2012.04310.x
  • Peer Reviewed
• [Journal Article] Impact of venous-systemic oxygen persufflation with nitric oxide gas on steatotic grafts after partial orthotopic liver transplantation in rats. (2013)
  • Author(s): Nagai K, Yagi S, et al.
  • Journal Title: Transplantation Volume: 95(1) Issue: 1 Pages: 78-84
  • DOI: 10.1097/tp.0b013e318277e2d1
  • Peer Reviewed
• [Journal Article] Small-for-size syndrome in living donor liver transplantation. (2012)
  • Author(s): Yagi S, Uemoto S.
  • Journal Title: Hepatobiliary Pancreat Dis Int. Volume: 11(6) Pages: 570-576
  • NAID: 120005241340
  • Peer Reviewed
• [Journal Article] Effect of portal haemodynamics on liver graft and intestinal mucosa after small-for-size liver transplantation in swine. (2012)
  • Author(s): Yagi S, et al.
  • Journal Title: Eur Surg Res. Volume: 48(3) Issue: 3 Pages: 163-170
  • DOI: 10.1159/000338622
  • Peer Reviewed
• [Journal Article] Pre- and perioperative factors affecting infection after living donor liver transplantation. (2012)
  • Author(s): Kaido T, Yagi S, et al.
  • Journal Title: Nutrition. Volume: 28(11-12) Issue: 11-12 Pages: 1104-1108
  • DOI: 10.1016/j.nut.2012.02.007
  • NAID: 120004873908
  • Peer Reviewed
• [Journal Article] Effects of Post-transplant Enteral Nutrition with an Immunomodulating Diet Containing Hydrolyzed Whey Peptide after Liver Transplantation (2012)
  • Author(s): Kaido T, Yagi S(5人略6番目)
  • Journal Title: World J Surg Volume: (In press) Issue: 7 Pages: 1666-1671
  • DOI: 10.1007/s00268-012-1529-9
  • Peer Reviewed
• [Journal Article] Pediatric liver transplantation using reduced and hyper-reduced left lateral segment grafts: a 10-year single-center experience. (2012)
  • Author(s): Shehata MR, Yagi S, et al.
  • Journal Title: Am J Transplant. Volume: 12(12) Issue: 12 Pages: 3406-3413
  • DOI: 10.1111/j.1600-6143.2012.04268.x
  • Peer Reviewed
• [Journal Article] Standard of hepatic vein reconstruction with patch plasty using native portal vein in adult living donor liver transplantation (2012)
  • Author(s): Mori A, Kaido T, Ogura Y, Ogawa K, Hata K, Yagi S, Yoshizawa A, Isoda H, Shibata T, Uemoto S.
  • Journal Title: Liver Transpl Volume: 17巻(In press) Issue: 5 Pages: 602-607
  • DOI: 10.1002/lt.23387
  • Peer Reviewed
• [Presentation] Validation of the Algorithms for Resection for Hepatocellular Carcinoma in Japan: BCLC vs. Clinical Practice Guideline in Japan (2014)
  • Author(s): S Yagi,S Kaihara, K Uryuhara, R Hosotani
  • Organizer: American college of surgeons 2014
  • Place of Presentation: San Francisco
  • Year and Date: 2014-10-26 – 2014-10-30
• [Presentation] 肝細胞癌外科手術症例の検討ー予後因子と至適術式 (2014)
  • Author(s): 八木 眞太郎、貝原 聡、瓜生原 健嗣、細谷 亮
  • Organizer: 第69回日本消化器外科学会総会
  • Place of Presentation: 郡山市
  • Year and Date: 2014-07-16 – 2014-07-18
• [Presentation] A novel organ preservation technique for small-for-size graft in liver transplantation: Venous Syetemic Nitric Oxiside with Oxygen Gaseous Persufflation (2014)
  • Author(s): Yagi S, Nagai K, Pramod K, Mahmoud A, Uemoto S, Tolba R
  • Organizer: The 12th Congress of the international Society for Experimental Microsurgery
  • Place of Presentation: 京都市
  • Year and Date: 2014-04-11 – 2014-04-12
• [Presentation] 肝細胞癌切除手術における本邦ならびに欧米治療ガイドラインの検証 (2014)
  • Author(s): 八木 眞太郎、貝原 聡、瓜生原 健嗣、細谷 亮
  • Organizer: 日本外科学会
  • Place of Presentation: 京都市
  • Year and Date: 2014-04-03 – 2014-04-05
• [Presentation] 肝移植手術における門脈圧制御―門脈下大静脈圧較差の意義 (2013)
  • Author(s): 八木 眞太郎、海道 利実、小倉 靖弘、飯田 拓、堀 智英、吉澤 淳、小川 晃平、藤本 康弘、波多野 悦朗、森 章、上 本 伸二
  • Organizer: 第113回外科学会定期学術集会
  • Place of Presentation: 福岡国際会議場(福岡市)
• [Presentation] 肝細胞癌に対する系統的切除の検討 (2013)
  • Author(s): 八木眞太郎、貝原 聡、瓜生原 健嗣、岡田 和幸、山本 健人、姚 思遠、井ノ口 健太、細谷亮
  • Organizer: 第25回肝胆膵外科学会
  • Place of Presentation: ホテル東日本宇都宮(宇都宮市)
• [Presentation] Anatomic versus Non-anatomic Resection for Hepatocellular Carcinoma (2013)
  • Author(s): Yagi S, Kaihara S, Uryuhara K, Hosotani R
  • Organizer: 2013 American college of surgeons
  • Place of Presentation: Walter E. Washington Convention Center (Washinton DC, アメリカ合衆国)
• [Presentation] 脂肪肝グラフトを用いた部分肝移植における新規臓器保存法 VSOP-NO(venous-systemic oxygen persufflation with nitric oxide gas)の効果 (2013)
  • Author(s): 長井 和之、八木 真太郎、Tolba Rene、上本 伸二
  • Organizer: 第113回外科学会定期学術集会
  • Place of Presentation: 福岡国際会議場(福岡市)
• [Presentation] ラット心停止ドナー肝移植に対する一酸化窒素を用いた臓器保存効果 (2013)
  • Author(s): 影山 詔一、八木 眞太郎、久保田 豊成、岡村 裕輔、吉村 弥緒、平尾 浩史、田中 宏和、斉藤 俊一、秦 浩一郎、藤本 康弘、小川 晃平、小倉 靖弘、海道 利実、上本 伸二
  • Organizer: 第113回外科学会定期学術集会
  • Place of Presentation: 福岡国際会議場(福岡市)
• [Presentation] Small-for-size肝移植に対する一酸化窒素を使った臓器保存の効果 (2012)
  • Author(s): 八木真太郎ら
  • Organizer: 第112回日本外科学会学術集会
  • Place of Presentation: 幕張メッセ(千葉県)
• [Presentation] 肝移植の動脈再建に難渋した動脈奇形合併成人アラジール症候群の一例 (2012)
  • Author(s): 八木真太郎ら
  • Organizer: 第24回日本肝胆膵外科学会・学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
• [Presentation] 肝移植手術における門脈圧制御の重要性 (2012)
  • Author(s): 八木真太郎ら
  • Organizer: 第67回日本消化器外科学会総会
  • Place of Presentation: 富山国際会議場(富山県)
• [Presentation] 成人生体肝移植における門脈-下大静脈圧較差の意義 (2012)
  • Author(s): 八木真太郎ら
  • Organizer: 第48回日本移植学会総会
  • Place of Presentation: 愛知県産業労働センター(愛知県)
• [Presentation] Small-for-size 肝移植に対する一酸化窒素による臓器保存の効果 (2012)
  • Author(s): 八木真太郎ら
  • Organizer: 第30回日本肝移植研究会
  • Place of Presentation: ホテル日航福岡(福岡県)
• [Presentation] 成人生体肝移植における門脈-下大静脈圧較差の意義 (2012)
  • Author(s): 八木真太郎ら
  • Organizer: 第19回日本門脈圧亢進症学会
  • Place of Presentation: フォーシーズンズホテル椿山荘(東京都)
• [Book] Experimental Organ Transplantation (2013)
  • Author(s): Yagi S, Kobayashi E, Uemoto S
  • Publisher: Nova Science Publishers, Inc