The analysis of immunological status against ABO blood type antigens after ABO incompatible liver transplantation

• Project/Area Number: 24591879
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: Kyoto University
• Principal Investigator: YOSHIZAWA Atsushi 京都大学, 医学(系)研究科(研究院), 助教 (60457984)
• Research Collaborator: UEDA Daisuke 京都大学, 大学院医学研究科
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: 移植免疫 / 肝移植 / 抗体 / 血液型不適合 / 抗体関連型拒絶反応 / 抗体関連型拒絶 / ABO血液型 / 液性免疫

Outline of Final Research Achievements

The aim of this project is to prove that immunological response against blood groups antigens has decreased after ABO-incompatible (ABO-i) liver transplantation. We analyzed this phenomenon in the aspect of clinical findings and in-vitro examination.
Throgh the clinical findings, we have found that antibody titers against ABO antigens significantly decreased after ABO-i liver transplantation. We analyzed the re-transplantation across ABO-i cases and found that the decrease of antibody titier was not the result of absorption of antibodies in allograft liver but the result of immunological unresponsiveness against ABO blood group antigens.To prove the immunological unresponsiveness, the response of PBMC, peripheral blood mononuclear cells from the ABO-i liver transplant recipients was examined. The response was examined through immune-deficient mice. The result shows that PBMC from ABO-i liver transplant recipients have lost the responsibility against ABO blood type antigens.

Research Products

• [Journal Article] Significance of semiquantitative assessment of preformed donor-specific antibody using luminex single bead assay in living related liver transplantation (2013)
  • Author(s): Yoshizawa A, Egawa H, Yurugi K, Hishida R, Tsuji H, Ashihara E, Miyagawa-Hayashino A, Teramukai S, Maekawa T, Haga H, Uemoto S
  • Journal Title: Clin Dev Immunol Volume: 2013 Pages: 972705-972705
  • DOI: 10.1155/2013/972705
  • Peer Reviewed
• [Presentation] Unknown Autoantibodies in the Idiopathic Posttransplant Hepatitis (2014)
  • Author(s): 平田 義弘
  • Organizer: World Transplant Congress
  • Place of Presentation: San Francisco , US
  • Year and Date: 2014-07-26 – 2014-07-31
• [Presentation] Isohemagglutinins titer of ABO-incompatible liver re-transplantation in perioperative period (2014)
  • Author(s): 上田 大輔
  • Organizer: World Transplant Congress
  • Place of Presentation: San Francisco , US
  • Year and Date: 2014-07-26 – 2014-07-31
• [Presentation] ABO不適合再肝移植症例の検討 (2014)
  • Author(s): 上田 大輔
  • Organizer: 第114回日本外科学会
  • Place of Presentation: 京都
  • Year and Date: 2014-04-03 – 2014-04-05
• [Presentation] Six cases of ABO-incompatible liver re-transplantation from donors with the same ABO-incompatible antigens as the first donor (2013)
  • Author(s): 上田 大輔
  • Organizer: 13回 アジア移植学会
  • Place of Presentation: (国際会議場（京都）
• [Presentation] 血液型不適合肝移植におけるB細胞免疫寛容成立の検討 (2013)
  • Author(s): 上田 大輔
  • Organizer: 日本移植学会
  • Place of Presentation: (国際会議場（京都）
• [Presentation] The Impact of Donor Specific Antibodies on the Long-Term Liver Allograft after Pediatric Living Donor Liver Transplantation (2012)
  • Author(s): A.Yoshizawa
  • Organizer: 国際移植学会
  • Place of Presentation: International Congress Centre、ベルリン、ドイツ