Development of air way xenotransplant graft with extracellular matrix and cytokine

Research Project

Project/Area Number	24591881
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	General surgery
Research Institution	Kagawa University
Principal Investigator	GO TETSUHIKO 香川大学, 医学部附属病院, 講師 (50313656)
Co-Investigator(Kenkyū-buntansha)	MATSUURA Natsumi 香川大学医学部, 附属病院, 助教 (20572853) KASAI Yoshitaka 香川大学医学部, 附属病院, 助教 (40610085) YOKOMISE Hiroyasu 香川大学, 医学部, 教授 (80231728)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000) Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000) Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	再生医学 / 気道再建 / 異種移植 / 人工材料 / 再生医療 / 人工臓器 / 人口臓器
Outline of Final Research Achievements	We investigated the novel tissue material for airway repair. Pig trachea was decellularised by detergent - enzymatic method treatment into extracellular matrix (ECM) which was confirmed non-antigenicity. Patch material made from this ECM was applied as xenotransplant tissue graft to repair air way defect made on dog’s cervical trachea. ECM patch graft derived from pig was incorporated in heterologous host of dog without immunosuppression and air way defect was restored successfully. On the other hand, applying cytokines such as erythropoietin, G-CSF did not improve epithelialization on the ECM graft, which needs to be investigated further.