| Project/Area Number |
24591920
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
OHE Kenji 京都大学, 健康長寿社会の総合医療開発ユニット, 特定講師 (30419527)
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| Co-Investigator(Kenkyū-buntansha) |
UTSUMI Toshiaki 藤田保健衛生大学, 医学部乳腺外科, 教授 (10176711)
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| Co-Investigator(Renkei-kenkyūsha) |
MAYEDA Akila 藤田保健衛生大学, 総合医科学研究所, 教授 (50212204)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 乳腺外科学 / エストロゲン受容体 / 癌遺伝子産物 / 選択的スプライシング / RNA結合蛋白質 / RNAスプライシング / 乳癌 |
|---|
| Outline of Final Research Achievements |
We have found HMGA1a binds to RNA in a sequence-specific manner and induces alternative splicing. While investigating this function in the estrogen-receptor alpha(ERα)gene, we found a “decoy RNA” of HMGA1a reverses its effect on ERα when stable transfectants of MCF-7 mammary carcinoma cells form tumors in nude mice. Since the expression of miR-16, which is known to downregulate HMGA1a, is decreased in these tumors, and has a strong homology to HMGA1a RNA binding sequence in its seed sequence, we hypothesized HMGA1a regulates its own regulating micro-RNA by its sequence-specific RNA binding function. This implies a positive feedback mechanism of HMGA1a which is known as oncogenic protein product.
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