Regulation of breast cancer stem cells by tumor microenvironment and analysis of target for therapy
| Project/Area Number |
24591929
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Research Institute for Clinical Oncology, Saitama Cancer Center |
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Principal Investigator |
YAMAGUCHI Yuri 埼玉県立がんセンター(臨床腫瘍研究所), その他部局等, 研究員 (80166628)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 乳癌 / ホルモン療法 / 癌微小環境 / 癌幹細胞 / 微小環境 / CAF / 耐性 / 癌 / 細胞・組織 / シグナル伝達 |
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| Outline of Final Research Achievements |
Estrogen and various growth factors are present in the breast cancer microenvironment, but their effects on breast cancer stem cells and the sensitivity to hormonal therapy remain unclear. We first analyzed the presence or absence of estrogen receptor (ER) in breast cancer stem like-cells derived from ER-positive MCF-7-E10 breast cancer cells transfected with ERE-GFP. Breast cancer stem like-cells express ER that could be activated by estrogen. Carcinoma-associated fibroblasts obtained from breast cancer tissues stimulate mammosphere growth of breast cancer stem like-cells derived from MCF-7-E10 cells. To analyze the mechanisms of in vivo development of estrogen-depletion resistance (EDR), we established several EDR cell lines from xenografts of MCF-7-E10 cells in ovariectomized SCID mice. ER expressions are decreased in some of EDR cell lines. ER positive EDR cell lines have more breast cancer stem like-cells than ER-negative ones.
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Report
(4 results)
Research Products
(45 results)
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[Journal Article] Androgen metabolite- dependent growth of hormone receptor- positive breast cancer as a possible aromatase inhibitor-resistance mechanism2013
Author(s)
Hanamura T, Niwa T, Nishikawa S, Konno H, Gohno T, Tazawa C, Kobayashi Y, Kurosumi M, Takei H, Yamaguchi Y, Ito K, Hayashi S
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Journal Title
Breast Cancer Res. Treat
Volume: 139(3)
Issue: 3
Pages: 731-740
DOI
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Peer Reviewed
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[Presentation] The androgen metabolite-dependent growth of hormone receptor positive breast cancer as a possible aromatase inhibitor-resistance mechanism2013
Author(s)
Toru Hanamura, Toshifumi Niwa, Sayo Nishikawa, Hiromi Konno, Tatsuyuki Gohno, Chika Chika Tazawa, Yasuhito Kobayashi, Masafumi Kurosumi, Hiroyuki Takei, Yuri Yamaguchi, Ken-ichi Ken-ichi Ito, Shin-ichi Hayashi
Organizer
AACR
Place of Presentation
Washington
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[Presentation] The androgen metabolite-dependent growth in hormone receptor positive breast cancer as a novel aromatase inhibitor-resistance mechanism2012
Author(s)
Hanamura T., Niwa1 T., Nishikawa1 S., Konno H., Ghono H., Kobayashi Y., , Kurosumi M., Takei, H., Yamaguchi Y. Ito Ken-ichi, Hayashi S.I,
Organizer
San Antonio Breast Cancer Symposium
Place of Presentation
Texsas,USA
Related Report
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