| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Estrogen and various growth factors are present in the breast cancer microenvironment, but their effects on breast cancer stem cells and the sensitivity to hormonal therapy remain unclear. We first analyzed the presence or absence of estrogen receptor (ER) in breast cancer stem like-cells derived from ER-positive MCF-7-E10 breast cancer cells transfected with ERE-GFP. Breast cancer stem like-cells express ER that could be activated by estrogen. Carcinoma-associated fibroblasts obtained from breast cancer tissues stimulate mammosphere growth of breast cancer stem like-cells derived from MCF-7-E10 cells. To analyze the mechanisms of in vivo development of estrogen-depletion resistance (EDR), we established several EDR cell lines from xenografts of MCF-7-E10 cells in ovariectomized SCID mice. ER expressions are decreased in some of EDR cell lines. ER positive EDR cell lines have more breast cancer stem like-cells than ER-negative ones.
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