Application of REIC/Dkk-3 gene therapy on gastrointestinal cancers targeting cancer stem cells
Project/Area Number |
24591943
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Digestive surgery
|
Research Institution | Okayama University of Science |
Principal Investigator |
KATAOKA Ken 岡山理科大学, 理学部, 准教授 (10293317)
|
Co-Investigator(Kenkyū-buntansha) |
SAKAGUCHI Masakiyo 岡山大学, 医歯薬学総合研究科, 准教授 (70379840)
MURATA Hitoshi 岡山大学, 医歯薬学総合研究科, 助教 (90579096)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | REIC/Dkk-3 / がん遺伝子治療 / 腸管上皮組織 / 皮膚組織 / TNF-α / 炎症 / がん幹細胞 / 組織幹細胞 |
Outline of Final Research Achievements |
Detailed screening of REIC/Dkk-3 expression revealed that REIC/Dkk-3 was localized in stem cells niche of skin and intestinal epithelium. REIC/Dkk-3 expression was also observed in three-dimensional cultured Caco-2 spheroids. To identify factor(s) regulating REIC/Dkk-3 expression in keratinocytes, we screened growth factors and cytokines that were reported to involved in keratinocyte growth and differentiation. Only TNF-alpha could down-regulate REIC/Dkk-3 in normal skin keratinocytes among seven factors we screened. The data suggest that expression balance of REIC/Dkk-3 and TNF-alpha adjust the skin tissue (re)modeling by skin keratinocytes and endothelial cells.
|
Report
(4 results)
Research Products
(15 results)
-
[Journal Article] DNAX-activating protein 10 (DAP10) membrane adaptor associates with receptor for advanced glycation end products (RAGE) and modulates the RAGE-triggered signaling pathway in human keratinocytes2014
Author(s)
Sakaguchi M, Murata H, Aoyama Y, Hibino T, Putranto EW, Ruma IM, Inoue Y, Sakaguchi Y, Yamamoto K, Kinoshita R, Futami J, Kataoka K, Iwatsuki K, Huh NH
-
Journal Title
J Biol Chem
Volume: 289
Issue: 34
Pages: 23389-23402
DOI
NAID
Related Report
Peer Reviewed / Open Access
-
-
-
[Journal Article] Design, synthesis and in vitro apoptotic activity of a series of α-benzyl phenylpropanoic acid-type peroxisome proliferator-activated receptor (PPAR) gamma partial agonists with improved aqueous solubility2013
Author(s)
Ohashi M, Oyama T, Putranto EW, Waku T, Nobusada H, Kataoka K, Matsuno K, Yashiro M, Morikawa K, Huh NH, Miyachi H
-
Journal Title
Bioorg Med Chem
Volume: 21
Pages: 2319-2332
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-