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Identification of new specific genes in esophageal and gastric cancer stem cells by SAGE method and analyses of the gene functions

Research Project

Project/Area Number 24591944
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Digestive surgery
Research InstitutionHiroshima University

Principal Investigator

HAMAI Yoichi  広島大学, 原爆放射線医科学研究所, 助教 (90423384)

Co-Investigator(Kenkyū-buntansha) OKADA Morihito  広島大学, 原爆放射線医科学研究所, 教授 (70446045)
Research Collaborator HIHARA Jun  広島大学, 原爆放射線医科学研究所, 講師 (10322744)
FURUKAWA Takaoki  広島大学, 病院, 医科診療医 (00736530)
YAMAKITA Ichiko  広島大学, 病院, 医科診療医 (70634088)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords癌幹細胞 / 食道癌 / 胃癌 / SAGE法
Outline of Final Research Achievements

We analyzed the function of specific gene selected by serial analysis of gene expression method (SAGE) for gastric and esophageal cancer stem cell.
We firstly sorted the human gastric and esophageal cancer cell line due to markers of CD44+ or - by fluorescence-activated cell sorting (FACS), and separately collected each cells with CD44+ or CD44-. Subsequently, we exhaustively analyzed and compared the gene expressions of messenger ribonucleic acid (mRNA) derived from cells with CD44+ and CD44- marker. Analyses of gene functions for a promising specific gene in cancer stem cell (CD44+ cell) selected by SAGE method was performed by RNAi method, cell migration, cell invasion, and thiazolyl blue tetrazolium bromide(MTT) assay.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (22 results)

All 2015 2014 2013 2012

All Journal Article (20 results) (of which Peer Reviewed: 20 results,  Open Access: 5 results) Presentation (2 results)

  • [Journal Article] Results of Neoadjuvant Chemoradiotherapy With Docetaxel and 5-Fluorouracil Followed by Esophagectomy to Treat Locally Advanced Esophageal Cancer2015

    • Author(s)
      Hamai Y, Hihara J, Emi M, Murakami Y, Kenjo M, Nagata Y, Okada M
    • Journal Title

      Ann Thorac Surg

      Volume: in press Issue: 6 Pages: 1887-1893

    • DOI

      10.1016/j.athoracsur.2015.02.042

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Emergency escape surgery for a gastro-bronchial fistula with respiratory failure that developed after esophagectomy2015

    • Author(s)
      Ibuki Y, Hamai Y, Hihara J, Taomoto J, Kishimoto I, Miyata Y, Okada M
    • Journal Title

      Surg Today

      Volume: 45 Issue: 3 Pages: 369-373

    • DOI

      10.1007/s00595-013-0821-0

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of neoadjuvant chemoradiotherapy on postoperative morbidity and mortality associated with esophageal cancer2014

    • Author(s)
      Hamai Y, Hihara J, Taomoto J, Yamakita I, Ibuki Y, Okada M
    • Journal Title

      Dis Esophagus

      Volume: in press Issue: 4 Pages: 358-364

    • DOI

      10.1111/dote.12207

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hemoglobin level influences tumor response and survival after neoadjuvant chemoradiotherapy for esophageal squamous cell carcinoma2014

    • Author(s)
      Hamai Y, Hihara J, Taomoto J, Yamakita I, Ibuki Y, Okada M
    • Journal Title

      World J Surg

      Volume: 38 Issue: 8 Pages: 2046-2051

    • DOI

      10.1007/s00268-014-2486-2

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lung Abscess Presenting as Tension Pyopneumothorax in a Gastrointestinal Cancer Patient2014

    • Author(s)
      Okita R, Miyata Y, Hamai Y, Hihara J, Okada M
    • Journal Title

      Annals of Thoracic and Cardiovascular Surgery

      Volume: 20 Issue: Supplement Pages: 478-481

    • DOI

      10.5761/atcs.cr.12-02195

    • NAID

      130003387996

    • ISSN
      1341-1098, 2186-1005
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hemoglobin Level Influences Tumor Response and Survival After Neoadjuvant Chemoradiotherapy for Esophageal Squamous Cell Carcinoma2014

    • Author(s)
      Hamai Y, Hihara J, Taomoto J, Yamakita I, Ibuki Y, Okada M
    • Journal Title

      World J Surg

      Volume: in press

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Emergency escape surgery for a gastro-bronchial fistula with respiratory failure that developed after esophagectomy2014

    • Author(s)
      Ibuki Y, Hamai Y, Hihara J, Taomoto J, Kishimoto I, Miyata Y, Okada M
    • Journal Title

      Surg Today

      Volume: in press

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Lung Abscess Presenting as Tension Pyopneumothorax in a Gastrointestinal Cancer Patient2014

    • Author(s)
      Okita R, Miyata Y, Hamai Y, Hihara J, Okada M
    • Journal Title

      Ann Thorac Cardiovasc Surg

      Volume: in press

    • NAID

      130003387996

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Esophageal bypass operation prior to definitive chemoradiotherapy in advanced esophageal cancer with tracheobronchial invasion2014

    • Author(s)
      Hihara J, Hamai Y, Emi M, Aoki Y, Taomoto J, Miyata Y, Okada M
    • Journal Title

      Ann Thorac Surg.

      Volume: 97 Issue: 1 Pages: 290-5

    • DOI

      10.1016/j.athoracsur.2013.08.060

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Involvement of homologous recombination in the synergism between cisplatin and poly(ADP-ribose) polymerase inhibition2013

    • Author(s)
      Sakogawa K, Aoki Y, Misumi K, Hamai Y, Emi M, Hihara J, Shi L, Kono K, Horikoshi Y, Sun J, Ikura T, Okada M, Tashiro S
    • Journal Title

      Cancer Sci. 2013 Sep 5

      Volume: 104 Issue: 12 Pages: 1593-1609

    • DOI

      10.1111/cas.12281

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Treatment outcomes and prognostic factors for thoracic esophageal cancer with clinical evidence of adjacent organ invasion2013

    • Author(s)
      Hamai Y, Hihara J, Emi M, Taomoto J, Aoki Y, Kishimoto I, Ibuki Y, Okada M
    • Journal Title

      Anticancer Res

      Volume: 33 Pages: 3495-502

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Involvement of ribonucleotide reductase-M1 in 5-fluorouracil induced DNA damage in esophageal cancer cell lines.2013

    • Author(s)
      Aoki Y, Sakogawa K, Hihara J, Emi M, Hamai Y, Kono K, Shi L, Sun J, Kitao H, Ikura T, Niida H, Nakanishi M, Okada M, Tashiro S.
    • Journal Title

      Int J Oncol.

      Volume: 42(6) Issue: 6 Pages: 1951-60

    • DOI

      10.3892/ijo.2013.1899

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Airway stenting for tracheal obstruction due to lymph node metastasis of hepatocellular carcinoma2013

    • Author(s)
      Hamai Y, Hihara J, Aoki Y, Taomoto J, Kishimoto I, Kobayashi Y, Miyata Y, Aikata H, Chayama K, Okada M
    • Journal Title

      Anticancer Res.

      Volume: 33 Pages: 1761-4

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Airway stenting for tracheal obstruction due to lymph node metastasis of hepatocellular carcinoma.2013

    • Author(s)
      Hamai Y, Hihara J, Aoki Y, Taomoto J, Kishimoto I, Kobayashi Y, Miyata Y, Aikata H, Chayama K, Okada M.
    • Journal Title

      Anticancer Res.

      Volume: 33 Pages: 1761-1764

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Advanced esophageal cancer with an esophago-bronchial fistula successfully treated by chemoradiotherapy following esophageal bypass surgery: report of a case.2012

    • Author(s)
      Aoki Y, Hihara J, Sakogawa K, Taomoto J, Hamai Y, Emi M, Okada M.
    • Journal Title

      Surg Today.

      Volume: 42 Issue: 11 Pages: 1088-1090

    • DOI

      10.1007/s00595-012-0323-5

    • NAID

      10031120772

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Leiomyosarcoma of the sigmoid colon with multiple liver metastases and gastric cancer: a case report.2012

    • Author(s)
      Hamai Y, Hihara J, Emi M, Aoki Y, Kushitani K, Tanabe K, Okada M.
    • Journal Title

      BMC Gastroenterol.

      Volume: 12 Issue: 1 Pages: 98-98

    • DOI

      10.1186/1471-230x-12-98

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Airway stenting for malignant respiratory complications in esophageal cancer.2012

    • Author(s)
      Hamai Y, Hihara J, Emi M, Aoki Y, Miyata Y, Okada M.
    • Journal Title

      Anticancer Res.

      Volume: 32 Pages: 1785-1790

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Laparoscopic thoracic duct clipping for persistent chylothorax after extrapleural pneumonectomy.2012

    • Author(s)
      Tsubokawa N, Hamai Y, Hihara J, Emi M, Miyata Y, Okada M.
    • Journal Title

      Ann Thorac Surg.

      Volume: 93 Issue: 5 Pages: e131-e132

    • DOI

      10.1016/j.athoracsur.2011.11.070

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Neoadjuvant chemoradiotherapy with docetaxel, cisplatin, and 5-fluorouracil for esophageal cancer.2012

    • Author(s)
      Manabu Emi
    • Journal Title

      Cancer chemotherapy and pharmacology

      Volume: 69 Issue: 6 Pages: 1499-1505

    • DOI

      10.1007/s00280-012-1853-7

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Esophageal reconstruction using the terminal ileum and right colon in esophageal cancer surgery2012

    • Author(s)
      Hamai Y, Hihara J, Emi M, et al
    • Journal Title

      Surg Today

      Volume: 42 Issue: 4 Pages: 342-350

    • DOI

      10.1007/s00595-011-0103-7

    • NAID

      10030546263

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Pre-therapeutic hemoglobin level has a significant impact on tumor response and outcome in neoadjuvant chemoradiotherapy for esophageal squamous cell carcinoma2013

    • Author(s)
      Yoichi Hamai, Jun Hihara, Junya Taomoto, Ichiko Kishimoto, Yuta Ibuki, Morihito Okada
    • Organizer
      The European Cancer Congress 2013
    • Place of Presentation
      Amsterdam, Netherlands
    • Related Report
      2013 Research-status Report
  • [Presentation] Effects of neoadjuvant chemoradiotherapy on postoperative morbidity and mortality associated with esophageal cancer2012

    • Author(s)
      Yoichi Hamai, Jun Hihara, Manabu Emi, Yoshiro Aoki, Morihito Okada
    • Organizer
      European society for medical oncology 2012 congress
    • Place of Presentation
      Vienna, Austria
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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