Identification of new specific genes in esophageal and gastric cancer stem cells by SAGE method and analyses of the gene functions
| Project/Area Number |
24591944
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Hiroshima University |
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Principal Investigator |
HAMAI Yoichi 広島大学, 原爆放射線医科学研究所, 助教 (90423384)
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| Co-Investigator(Kenkyū-buntansha) |
OKADA Morihito 広島大学, 原爆放射線医科学研究所, 教授 (70446045)
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| Research Collaborator |
HIHARA Jun 広島大学, 原爆放射線医科学研究所, 講師 (10322744)
FURUKAWA Takaoki 広島大学, 病院, 医科診療医 (00736530)
YAMAKITA Ichiko 広島大学, 病院, 医科診療医 (70634088)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 癌幹細胞 / 食道癌 / 胃癌 / SAGE法 |
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| Outline of Final Research Achievements |
We analyzed the function of specific gene selected by serial analysis of gene expression method (SAGE) for gastric and esophageal cancer stem cell.
We firstly sorted the human gastric and esophageal cancer cell line due to markers of CD44+ or － by fluorescence-activated cell sorting (FACS), and separately collected each cells with CD44+ or CD44－. Subsequently, we exhaustively analyzed and compared the gene expressions of messenger ribonucleic acid (mRNA) derived from cells with CD44+ and CD44－ marker. Analyses of gene functions for a promising specific gene in cancer stem cell (CD44+ cell) selected by SAGE method was performed by RNAi method, cell migration, cell invasion, and thiazolyl blue tetrazolium bromide(MTT) assay.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Involvement of homologous recombination in the synergism between cisplatin and poly(ADP-ribose) polymerase inhibition2013
Author(s)
Sakogawa K, Aoki Y, Misumi K, Hamai Y, Emi M, Hihara J, Shi L, Kono K, Horikoshi Y, Sun J, Ikura T, Okada M, Tashiro S
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Journal Title
Cancer Sci. 2013 Sep 5
Volume: 104
Issue: 12
Pages: 1593-1609
DOI
Related Report
Peer Reviewed
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[Journal Article] Involvement of ribonucleotide reductase-M1 in 5-fluorouracil induced DNA damage in esophageal cancer cell lines.2013
Author(s)
Aoki Y, Sakogawa K, Hihara J, Emi M, Hamai Y, Kono K, Shi L, Sun J, Kitao H, Ikura T, Niida H, Nakanishi M, Okada M, Tashiro S.
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Journal Title
Int J Oncol.
Volume: 42(6)
Issue: 6
Pages: 1951-60
DOI
Related Report
Peer Reviewed
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