| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
DNA methylation in the long interspersed nucleotide element-1, L1 (LINE-1) repetitive element is a good indicator of the global DNA methylation level. In some types of human neoplasms, However, prognostic significance of LINE-1 hypomethylaiton in gastric cancer remains unclear.Using 203 resected gastric cancer specimens, we quantified LINE-1 methylation using bisulfite-pyrosequencing technology. Gastric cancers showed significantly lower LINE-1 methylation levels compared to matched normal gastric mucosa. LINE-1 hypomethylation was significantly associated with shorter overall survival. LINE-1 hypomethylation in gastric cancer is associated with shorter survival, suggesting that it has potential for use as a prognostic biomarker. LINE-1 methylation in the noncancerous gastric mucosae of gastric cancer patients was significantly lower than in the gastric mucosae of the autopsied individuals suggesting the formation of epigenetic field defect in noncancerous gastric mucosae.
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