| Project/Area Number |
24591953
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Oita University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SHIRAISHI Norio 大分大学, 医学部, 教授 (20271132)
INOMATA Masafumi 大分大学, 医学部, 教授 (60315330)
NISHIZONO Akira 大分大学, 医学部, 教授 (70218155)
OHTA Masayuki 大分大学, 医学部, 准教授 (80271104)
KITANO Seigo 大分大学, 法人本部, 学長 (90169871)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | レオウイルス / 微小転移 / 蛍光プローブ |
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| Outline of Final Research Achievements |
Oncolytic reovirus is susceptibility to cells with the activated Ras signaling pathway. The oncolytic efficacy of reovirus has been demonstrated in several cancer experimental models. We evaluated the functional activity of reovirus labeled with Alexa Fluor 488, a stable fluorescent dye, as a new tracer for sentinel navigation technique. MKN45p human gastric cancer cell line and reovirus serotype 3 were used in this study. The cytopathic effect and morphological change after reovirus labeled with Alexa Fluor 488 infection were examined.
After the labeled reovirus infection, cytopathic effect was observed in MKN45p cell line, but not in control cell. In addition, severe morphological changes were also seen especially in the infected cancer cell line. Reovirus labeled with Alexa Fluor 488 could specifically infect a human gastric cancer cell line. This labeled virus might act as a novel tracer for sentinel node navigation surgery of gastric cancer.
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