| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Chemokines and their receptors play key roles in leukocyte trafficking and are also implicated in cancer metastasis. By constructing SW620 cell lines with reduced expression of CXCR3 and/or CXCR4 using microRNA, we investigated in vivo metastatic activities in a mouse rectal transplantation model. CXCR3-, CXCR4-, and CXCR3/CXCR4 double-knockdowns significantly reduced metastasis to lymph nodes, liver and lungs, compared with the control. Importantly, its suppressive effect was significantly stronger in CXCR3/CXCR4 double-knockdowns. Clinical specimens of liver metastasis showed a strong inverse correlation between levels of CCL15 and Smad4. Patients with CCL15-expressing metastases showed significantly shorter times of disease-free survival than those with CCL15-negative metastases.
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