Predictive diagnostics effect and clinical application of circulating cancer stem-like cells in colon cancer patients treated with molecular targeted therapy

• Project/Area Number: 24591984
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Teikyo University
• Principal Investigator: IINUMA HISAE 帝京大学, 医学部, 講師 (30147102)
• Co-Investigator(Kenkyū-buntansha): WATANABE Toshiaki 東京大学, 医学部, 教授 (80210920) ; YAMADA Hideki 帝京大学, 医学部, 助手 (50525269) ; HAYAMA Tamuro 帝京大学, 医学部, 助手 (80533744)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 循環腫瘍細胞 / 癌幹細胞 / 大腸癌 / 転移診断 / 予後予測 / ニッチ / 肝転移 / 骨髄転移 / バイオマーカー / 再発予測 / 治療効果予測

Outline of Final Research Achievements

Recently, we make clear that the some population of CTCs expressing the cancer stem cells marker, and named these cells as circulating tumor stem cell (CTSC). Here we aimed to clarify the clinical significance of CTSC and CTC (CTSC /CTC) in colorectal cancer patients. Multi-marker panel consist of CEA, epithelial markers (CK19, CK20), cancer stem cell marker (CD133, CD44) and new marker X were used for detection of CTSC/CTC.
In this study, we demonstrated that CTSC/CTC level was a significant prognostic factor for OS and DFS in patients with Dukes stage B and C cancer. Next, we assessed the value of CTSC/CTC levels as biomarkers for clinical response in advanced CRC patients treated with molecular target reagent (bevacizumab, cetuximab) and chemotherapy (FOLFOX, FOLFIRI). We demonstrated that CTSC/CTC levels of PB have a predictive value for clinical response and survival in patients treated with these combination therapies.

Research Products

• [Journal Article] Clinical significance of microRNA-21 as a biomarker in each Dukes’stage of colorectal cancer. (2015)
  • Author(s): Fukushima Y, Iinuma H, Tsukamoto M, Matsuda K, Hashiguchi Y
  • Journal Title: Oncol Rep. Volume: 33(2) Issue: 2 Pages: 573-582
  • DOI: 10.3892/or.2014.3614
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Synchronous colorectal malignancy and abdominal aortic aneurysm treated with endovascular aneurysm repair followed by laparoscopic colectomy. (2015)
  • Author(s): Kawai K, Sunami E, Tanaka J, Tanaka T, Kiyomatsu T, Nozawa H, Kazama S, Kanazawa T, Hosaka A, Ishihara S, Yamaguchi H, Shigematsu K, Watanabe T.
  • Journal Title: Int Surg. Volume: 100(4) Issue: 4 Pages: 600-603
  • DOI: 10.9738/intsurg-d-14-00111.1
  • Peer Reviewed
• [Journal Article] Impact of revisions of the JSCCR guidelines on the treatment of T1 colorectal carcinomas in Japan. (2015)
  • Author(s): Urabe Y, Tanaka S, Saito Y, Igarashi M, Watanabe T, Sugihara K.
  • Journal Title: Z Gastroenterol. Volume: 53(4) Pages: 291-301
  • Peer Reviewed
• [Journal Article] Local recurrence after endoscopic resection for large colorectal neoplasia: a multicenter prospective study in Japan. (2015)
  • Author(s): Oka S, Tanaka S, Saito Y, Iishi H, Kudo SE, Ikematsu H, Igarashi M, Saitoh Y, Inoue Y, Kobayashi K, Hisabe T, Tsuruta O, Sano Y, Yamano H, Shimizu S, Yahagi N, Watanabe T,Sugihara K
  • Journal Title: Am J Gastroenterol. Volume: 110(5) Issue: 5 Pages: 697-707
  • DOI: 10.1038/ajg.2015.96
  • Peer Reviewed
• [Journal Article] Japanese Society for Cancer of the Colon and Rectum (JSCCR) Guidelines 2014 for treatment of colorectal cancer. (2015)
  • Author(s): Watanabe T, Itabashi M, Shimada Y, Sugihara K
  • Journal Title: Int J Clin Oncol. Volume: 20(2) Issue: 2 Pages: 207-239
  • DOI: 10.1007/s10147-015-0801-z
  • Peer Reviewed
• [Journal Article] Relationship between smoking and multiple colorectal cancers in patients with Japanese Lynch syndrome: a cross-sectional study conducted by the Japanese Society for Cancer of the Colon and Rectum. (2015)
  • Author(s): Tanakaya K, Furukawa Y, Nakamura Y, Hirata K, Tomita N, Tamura K, Sugano K, Ishioka C, Yoshida T, Ishida H, Watanabe T, Sugihara K.
  • Journal Title: Jpn J Clin Oncol. Volume: 45(3) Issue: 3 Pages: 307-310
  • DOI: 10.1093/jjco/hyu218
  • Peer Reviewed / Open Access
• [Journal Article] Comparison of clinical features between suspected familial colorectal cancer type X and Lynch syndrome in Japanese patients with colorectal cancer: (2015)
  • Author(s): Yamaguchi T, Furukawa Y, Nakamura Y, Matsubara N, Ishikawa H, Arai M, Tomita N, Tamura K, Sugano K, Ishioka C, Yoshida T, Moriya Y, Ishida H, Watanabe T, Sugihara K;
  • Journal Title: Jpn J Clin Oncol. Volume: 45(2) Issue: 2 Pages: 153-159
  • DOI: 10.1093/jjco/hyu190
  • Peer Reviewed / Open Access
• [Journal Article] Patient factors predisposing to complications following laparoscopic surgery for colorectal cancers. (2015)
  • Author(s): Ishihara S, Matsuda K, Tanaka T, Tanaka J, Kiyomatsu T, Kawai K, Nozawa H, Kazama S, Kanazawa T, Yamaguchi H, Sunami E, Kitayama J, Hashiguchi Y, Watanabe T.
  • Journal Title: Surg Laparosc Endosc Percutan Tech. Volume: 25(2) Issue: 2 Pages: 168-172
  • DOI: 10.1097/sle.0000000000000110
  • Peer Reviewed
• [Journal Article] Detection of carcinoembryonic antigen mRNA in peritoneal lavage by the transcription-reverse transcription concerted method indicates poor prognosis in patients with stage II and III colon cancer. (2015)
  • Author(s): Murono K, Kazama S, Yamaguchi H, Kawai K, Ishihara S, Sunami E, Kitayama J, Satoh Y, Kurihara M, Yatomi Y, Watanabe T.
  • Journal Title: Surgery Volume: 157 Issue: 2 Pages: 322-30
  • DOI: 10.1016/j.surg.2014.09.015
  • Peer Reviewed
• [Journal Article] Phase I clinical study of multiple epitope peptide vaccine combined with chemoradiation therapy in esophageal cancer patients (2014)
  • Author(s): Iinuma H, Fukushima R, Inaba T,他全15名
  • Journal Title: J. Transl. Med Volume: 12(1) Issue: 1 Pages: 84-84
  • DOI: 10.1186/1479-5876-12-84
  • Peer Reviewed
• [Journal Article] Allelic Imbalance at an 8q24 Oncogenic SNP is Involved in Activating MYC in Human Colorectal Cancer (2014)
  • Author(s): Sugimachi K, Niida A, Yamamoto K, Shimamura T, Imoto S, Iinuma H, Shinden Y, Eguchi H, Sudo T, Watanabe M, Tanaka J, Kudo S, Hase K, Kusunoki M, Yamada K, Shimada Y, Sugihara K, Maehara Y, Miyano S, Mori M, Mimori K
  • Journal Title: Ann Surg Oncol Volume: 21 Issue: S4 Pages: 3468-3476
  • DOI: 10.1245/s10434-013-3468-6
  • Peer Reviewed / Open Access
• [Journal Article] Proximal shift of colorectal cancer along with aging. (2014)
  • Author(s): Iida Y, Kawai K, Tsuno NH, Ishihara S, Yamaguchi H, Sunami E, Kitayama J, Watanabe T.
  • Journal Title: Clin Colorectal Cancer Volume: 13 Issue: 4 Pages: 213-8
  • DOI: 10.1016/j.clcc.2014.06.005
  • Peer Reviewed
• [Journal Article] Prognostic impact of tumor location in stage IV colon cancer: a propensity score analysis in a multicenter study. (2014)
  • Author(s): Ishihara S, Nishikawa T2, Tanaka T2, Tanaka J2, Kiyomatsu T2, Kawai K2, Hata K2, Nozawa H2, Kanazawa T2, Kazama S2, Yamaguchi H2, Sunami E2, Kitayama J2, Hashiguchi Y3, Sugihara K4, Watanabe T5.
  • Journal Title: Int J Surg. Volume: 12(9) Issue: 9 Pages: 925-30
  • DOI: 10.1016/j.ijsu.2014.07.270
  • Peer Reviewed / Open Access
• [Journal Article] S-1 as adjuvant chemotherapy for stage III colon cancer: a randomized phase III study (ACTS-CC trial). (2014)
  • Author(s): Yoshida M, Ishiguro M, Ikejiri K, Mochizuki I, Nakamoto Y, Kinugasa Y, Takagane A, Endo T, Shinozaki H, Takii Y, Mochizuki H, Kotake K, Kameoka S, Takahashi K, Watanabe T, Watanabe M, Boku N, Tomita N, Nakatani E, Sugihara K
  • Journal Title: Ann Oncol. Volume: 25(9) Issue: 9 Pages: 1743-1749
  • DOI: 10.1093/annonc/mdu232
  • Peer Reviewed
• [Journal Article] A case of extraperitoneal stoma-associated internal hernia after abdominoperineal resection. (2014)
  • Author(s): Yokoyama Y, Kawai K, Kazama S, Yoneyama S, Tanaka J, Tanaka T, Kiyomatsu T, Nozawa H, Kanazawa T, Yamaguchi H, Ishihara S, Sunami E, Kitayama J, Watanabe T.
  • Journal Title: World J Surg Oncol. Volume: 12 Issue: 1 Pages: 141-141
  • DOI: 10.1186/1477-7819-12-141
  • Peer Reviewed
• [Journal Article] Urethral metastasis from a sigmoid colon carcinoma: a quite rare case report and review of the literature. (2014)
  • Author(s): Kazama S, Kitayama J, Sunami E, Niimi A, Nomiya A, Homma Y, Watanabe T.
  • Journal Title: BMC Surg. Volume: 14 Issue: 1 Pages: 31-31
  • DOI: 10.1186/1471-2482-14-31
  • Peer Reviewed
• [Journal Article] Barium enema and CT volumetry for predicting pathologic response to preoperative chemoradiotherapy in rectal cancer patients. (2014)
  • Author(s): Murono K, Kawai K, Tsuno NH, Ishihara S, Yamaguchi H, Sunami E, Kitayama J, Watanabe T.
  • Journal Title: Dis Colon Rectum. Volume: 57(6) Issue: 6 Pages: 715-24
  • DOI: 10.1097/dcr.0000000000000070
  • Peer Reviewed / Open Access
• [Journal Article] Changes in colorectal cancer care in japan before and after guideline publication: a nationwide survey about D3 lymph node dissection and adjuvant chemotherapy. (2014)
  • Author(s): Ishiguro M, Higashi T, Watanabe T, Sugihara K
  • Journal Title: J Am Coll Surg. Volume: 218(5) Issue: 5 Pages: 969-977
  • DOI: 10.1016/j.jamcollsurg.2013.12.046
  • Peer Reviewed / Open Access
• [Journal Article] Prediction of response to preoperative chemoradiotherapy in rectal cancer by using reverse transcriptase polymerase chain reaction analysis of four genes. (2014)
  • Author(s): Watanabe T, Kobunai T, Akiyoshi T, Matsuda K, Ishihara S, Nozawa K.
  • Journal Title: Dis Colon Rectum. Volume: 57(1) Issue: 1 Pages: 23-31
  • DOI: 10.1097/01.dcr.0000437688.33795.9d
  • Peer Reviewed
• [Journal Article] Clinical benefit of surgery for stage IV colorectal cancer with synchronous peritoneal metastasis. (2014)
  • Author(s): Kobayashi H, Kotake K, Funahashi K, Hase K, Hirata K, Iiai T, Kameoka S, Kanemitsu Y, Maeda K, Murata A, Ohue M, Shirouzu K, Takahashi K, Watanabe T, Yano H, Yatsuoka T, Hashiguchi Y, Sugihara K
  • Journal Title: J Gastroenterol. Volume: 49(4) Issue: 4 Pages: 646-654
  • DOI: 10.1007/s00535-013-0820-3
  • Peer Reviewed
• [Journal Article] Diagnosis and management of hereditary colorectal cancer according to the JSCCR Guidelines 2012 for the Clinical Practice of Hereditary Colorectal Cance (2014)
  • Author(s): Ishida H, Iwama T, Tomita N, Koizumi K, Akagi K, Ishiguro M, Watanabe T, Sugihara K.
  • Journal Title: Nihon Rinsho Volume: 72(1) Pages: 143-149
  • Peer Reviewed
• [Journal Article] Aberrant Expression of Plastin-3 Via Copy Number Gain Induces the Epithelial-Mesenchymal Transition in Circulating Colorectal Cancer Cells (2013)
  • Author(s): Sugimachi K, Yokobori T, Iinuma H, Ueda M, Ueo H, Shinden Y, Eguchi H, Sudo T, Suzuki A, Maehara Y, Mori M, Mimori K
  • Journal Title: Ann Surg Oncol Volume: 20 Issue: 11 Pages: 3366-3377
  • DOI: 10.1245/s10434-013-3366-y
  • Peer Reviewed
• [Journal Article] Contrasting expression patterns of histone mRNA and microRNA 760 in patients with gastric cancer. (2013)
  • Author(s): Iwaya T, Fukagawa T, Suzuki Y, Takahashi Y, Sawada G, Ishibashi M, Kurashige J, Sudo T, Tanaka F, Shibata K, Endo F, Katagiri H, Ishida K, Kume K, Nishizuka S, Iinuma H, Wakabayashi G, Mori M, Sasako M, Mimori K.
  • Journal Title: Clin Cancer Res Volume: 19 (23) Issue: 23 Pages: 6438-49
  • DOI: 10.1158/1078-0432.ccr-12-3186
  • Peer Reviewed
• [Journal Article] Plastin3 is a novel marker for circulating tumor cells undergoing the epithelial-mesenchymal transition and is associated with colorectal cancer prognosis. (2013)
  • Author(s): Yokobori T, Iinuma H, Shimamura T, Imoto S, Sugimachi K, Ishii H, Iwatsuki M, Ota D, Ohkuma M, Iwaya T, Nishida N, Kogo R, Sudo T, Tanaka F, Shibata K, Toh H, Sato T, Barnard GF, Fukagawa T, Yamamoto S, Nakanishi H, Sasaki S, Miyano S, Watanabe T, Kuwano H, Mimori K, Pantel K, Mori M.
  • Journal Title: Cancer Res Volume: 1; 73(7) Issue: 7 Pages: 2059-69
  • DOI: 10.1158/0008-5472.can-12-0326
  • Peer Reviewed
• [Journal Article] The rs6983267 SNP Is Associated with MYC Transcription Efficiency, Which Promotes Progression and Worsens Prognosis of Colorectal Cancer. (2013)
  • Author(s): Takatsuno Y
  • Journal Title: Ann Surg Oncol Volume: 20 Issue: 4 Pages: 1395-402
  • DOI: 10.1245/s10434-012-2657-z
  • Peer Reviewed
• [Journal Article] Plastin3 Is a Novel Marker for Circulating Tumor Cells Undergoing the Epithelial-Mesenchymal Transition and Is Associated with Colorectal Cancer Prognosis. (2013)
  • Author(s): Yokobori T, Iinuma H, Shimamura T et al.
  • Journal Title: Cancer Res Volume: 73(7) Pages: 2059-2069
  • Peer Reviewed
• [Journal Article] Accurate and rapid novel genetic diagnosis for detection of sentinel lymph node metastasis in breast cancer patients. (2012)
  • Author(s): Iinuma H, Tamura J, Omoto D et al.
  • Journal Title: Br J Cancer Volume: 107(4) Issue: 4 Pages: 724-731
  • DOI: 10.1038/bjc.2012.300
  • Peer Reviewed
• [Journal Article] Multicenter, phase II clinical trial of cancer vaccination for advanced esophageal cancer with three peptides derived from novel cancer-testis antigens. (2012)
  • Author(s): Kono K
  • Journal Title: J Transl Med Volume: 10（141） Issue: 1 Pages: 141-141
  • DOI: 10.1186/1479-5876-10-141
  • Peer Reviewed
• [Journal Article] Increased Risk for CRC in Diabetic Patients with the Nonrisk Allele of SNPs at 8q24 (2012)
  • Author(s): Ishimaru S, Iinuma H, et al
  • Journal Title: Ann Surg Oncol Volume: 21(in press) Issue: 9 Pages: 21-21
  • DOI: 10.1245/s10434-012-2278-6
  • Peer Reviewed
• [Journal Article] Chromosomal Instability (CIN) Phenotype, CIN High or CIN Low, Predicts Survival for Colorectal Cancer (2012)
  • Author(s): Watanabe T, Iinuma H, et al
  • Journal Title: J Clin Oncol Volume: 30(in press) Issue: 18 Pages: 2256-64
  • DOI: 10.1200/jco.2011.38.6490
  • Peer Reviewed
• [Journal Article] Clinicopathological and prognostic significance of microRNA-107 and its relationship to DICER1 mRNA expression in gastric cancer (2012)
  • Author(s): Inoue T, Iinuma H, et al
  • Journal Title: Oncol Rep Volume: 27 Pages: 1759-64
  • DOI: 10.3892/or.2012.1709
  • Peer Reviewed
• [Journal Article] Prognostic significance of CTCs and CSCs of tumor drainge vein blood in Dukes' stage B and C colorectal cancer (2012)
  • Author(s): Shimada R, Iinuma H, et al
  • Journal Title: Oncol Rep Volume: 27 Issue: 4 Pages: 947-53
  • DOI: 10.3892/or.2012.1649
  • Peer Reviewed
• [Journal Article] Prognostic significance of PDCD4 expression and association with microRNA-21 in each Dukes' stage of colorectal cancer patients (2012)
  • Author(s): Horiuchi A, Iinuma H, et al
  • Journal Title: Oncol Rep Volume: 27 Pages: 1384-92
  • DOI: 10.3892/or.2012.1648
  • Peer Reviewed
• [Journal Article] 2cm以下の大腸進行癌の特徴 (2012)
  • Author(s): 飯沼久恵、石原聡一郎、渡邉聡明
  • Journal Title: INTESTINE Volume: 16 (4) Pages: 331-336
• [Journal Article] 微量癌細胞研究の展開 (2012)
  • Author(s): 飯沼久恵、渡邉聡明
  • Journal Title: 日本外科学会誌 Volume: 113(2) Pages: 177-184
  • NAID: 110009425990
• [Journal Article] 大腸癌Dukes' Stage BおよびC症例における腫瘍還流血中遊離癌細胞のバイオマーカーとしての有用性 (2012)
  • Author(s): 島田竜, 飯沼久恵, 田村純子
  • Journal Title: 癌と化学療法 Volume: 39(12) Pages: 1763-1765
• [Presentation] 大腸癌ステージ別におけるmicroRNA-21の予後予測バイオマーカーとしての有用性 (2014)
  • Author(s): 福島慶久、飯沼久恵、田村純子、塚本充雄、赤羽根拓弥、島田竜、中村圭介、土屋剛史、藤井正一、野澤慶次郎、松田圭二、橋口陽二郎
  • Organizer: 第２７回日本バイオセラピィ学会集会総会
  • Place of Presentation: 大阪
  • Year and Date: 2014-12-04 – 2014-12-05
• [Presentation] 大腸癌血漿エクソソーム由来microRNA-21検出の臨床的意義と予後予測診断の有用性の検討 (2014)
  • Author(s): 田村純子、飯沼久恵、塚本充雄、福島慶久、赤羽根拓弥、中村圭介、島田竜、土屋剛史、野澤慶次郎、藤井正一、松田圭二、橋口陽二郎
  • Organizer: 第２７回日本バイオセラピィ学会集会総会
  • Place of Presentation: 大阪
  • Year and Date: 2014-12-04 – 2014-12-05
• [Presentation] 大腸癌組織におけるDicer1mRNAおよびDrosha mRNA発現の臨床的意義 (2014)
  • Author(s): 赤羽根拓弥、飯沼久恵、田村純子、塚本充雄、島田竜、中村圭介、藤井正一、野澤慶次郎、松田圭二、橋口陽二郎
  • Organizer: 第２７回日本バイオセラピィ学会集会総会
  • Place of Presentation: 大阪
  • Year and Date: 2014-12-04 – 2014-12-05
• [Presentation] 食道癌ペプチドワクチン療法におけるＣＴＣの分子生物学的特徴とバイオマーカーとしての有用性 (2014)
  • Author(s): 飯沼久恵、田村純子、小川越史、堀川昌宏、矢口義久、稲葉毅、河野浩二、池田正、中村祐輔、福島亮治
  • Organizer: 第７３回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 循環血液中のエクソソーム内マイクロＲＮＡは消化器癌の有用なバイオマーカーとなる (2014)
  • Author(s): 杉町圭史、松村多恵、新田吉陽、井口友宏、江口英利。飯沼久恵、落合孝広、調 憲、前原喜彦、三森功士
  • Organizer: ７３回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 下部直腸癌の治療戦略―術前治療の意義とその効果予測 (2014)
  • Author(s): 橋口陽二郎、神藤英二、飯沼久恵、上野秀樹、長谷和生、藤井正一、野澤慶次郎、松田圭二
  • Organizer: 第７３回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 大腸癌患者における血漿エクソソーム由来microRNA-21のバイオマーカーとしての臨床的意義 (2014)
  • Author(s): 田村純子、飯沼久恵、福島慶久、赤羽根拓弥、中村圭介、土屋剛史、端山軍、藤井正一、野澤慶次郎、松田圭二、橋口陽二郎
  • Organizer: 第７３回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Phase I study of peptide vaccine following chemoradiation therapy against unresectable esophageal cancers patients. (2014)
  • Author(s): Iinuma H, Tamura J, Inoue T, Ogawa E, Horikawa M, Ikeda Y, Inaba T, Okinaga K, Yoshida K, Tsunoda T, Nakamura Y, Fukushima R
  • Organizer: The52nd Annual Meeting of Japan Society of Clinical Oncology
  • Place of Presentation: Yokohama
  • Year and Date: 2014-08-28 – 2014-08-30
• [Presentation] 食道癌ペプチドワクチン療法におけるＣＴＣのバイオマーカーとしての有用性 (2014)
  • Author(s): 飯沼久恵、田村純子、小川越史、井上泰介、堀川昌宏、矢口義久、池田佳史、稲葉毅、中村祐輔、福島亮治
  • Organizer: 第３５回癌免疫外科研究会
  • Place of Presentation: 大阪
  • Year and Date: 2014-05-22 – 2014-05-23
• [Presentation] 胃癌症例におけるDicer1発現と臨床病理学的因子および予後との関連性 (2014)
  • Author(s): 井上泰介、飯沼久恵、田村純子、小川越史、堀川昌宏、矢口義久、池田佳史、稲葉毅、福島亮治
  • Organizer: 第３５回癌免疫外科研究会
  • Place of Presentation: 大阪
  • Year and Date: 2014-05-22 – 2014-05-23
• [Presentation] 大腸癌血漿エクソソームにおけるmicroRNA-21の発現と臨床的意義 (2014)
  • Author(s): 田村純子、飯沼久恵、福島慶久、赤羽根拓弥、中村圭介、土屋剛史、端山軍、藤井正一、野澤慶次郎、松田圭二、橋口陽二郎
  • Organizer: 第３５回癌免疫外科研究会
  • Place of Presentation: 大阪
  • Year and Date: 2014-05-22 – 2014-05-23
• [Presentation] 大腸癌組織におけるmicroRNA-21の発現と臨床的意義 (2014)
  • Author(s): 福島慶久、飯沼久恵、田村純子、赤羽根拓弥、中村圭介、土屋剛史、端山軍、藤井正一、野澤慶次郎、松田圭二、橋口陽二郎
  • Organizer: 第３５回癌免疫外科研究会
  • Place of Presentation: 大阪
  • Year and Date: 2014-05-22 – 2014-05-23
• [Presentation] 大腸癌における遊離癌幹様細 を指標とした腹膜再発遺伝子診断の有用性 (2014)
  • Author(s): 飯沼久恵、田村純子、福島慶久、赤羽根拓弥、堀内敦、中村圭介、土屋剛史、山田英樹、端山軍、野澤慶次郎、藤井正一、松田圭二、橋口陽二郎
  • Organizer: 第114回日本外科学会定期学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-04-03 – 2014-04-05
• [Presentation] 大腸癌ステージ別によるmicroRNA-21の予後予測バイオマーカーとしての有用性 (2014)
  • Author(s): 福島慶久、飯沼久恵、田村純子、赤羽根拓弥、堀内敦、中村圭介、山田英樹、端山軍、土屋剛史、藤井正一、野沢慶次郎、松田圭二、橋口陽二郎
  • Organizer: 第114回日本外科学会定期学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-04-03 – 2014-04-05
• [Presentation] 胃癌の末梢血および脾臓細胞におけるMDSC細胞の免疫抑制機構 (2014)
  • Author(s): 飯沼久恵、田村純子、福島亮治、冲永功太
  • Organizer: 第２７回日本小児脾臓研究会
  • Place of Presentation: 熊本
  • Year and Date: 2014-04-01
• [Presentation] 大腸癌における血中循環癌細胞の検出と臨床的意義 (2013)
  • Author(s): 飯沼久恵、田村純子、福島慶久、赤羽根拓弥、島田竜、堀内敦、中村圭介、山田英樹、端山軍、野澤慶次郎、石原聡一郎、松田圭二、三森功士、渡邉聡明、森正樹、橋口陽二郎
  • Organizer: 第１１３回日本外科学会総会
  • Place of Presentation: 福岡
  • Invited
• [Presentation] 大腸癌におけるDICER1mRNAの発現と臨床病理学的因子および予後との関連性 (2013)
  • Author(s): 赤羽根拓弥、飯沼久恵、福島慶久、堀内敦、中村圭介、山田英樹、端山軍、野澤慶次郎、石原聡一郎、松田圭二、渡邉聡明、橋口陽二郎
  • Organizer: 第１１３回日本外科学会総会
  • Place of Presentation: 福岡
• [Presentation] 大腸癌における新規癌抑制遺伝子PDCD4 mRNA発現の意義とmicroRNA-21との関連性 (2013)
  • Author(s): 福島慶久、飯沼久恵、堀内敦、赤羽根拓弥、中村圭介、島田竜、山田英樹、端山軍、野澤慶次郎、石原聡一郎、松田圭二、渡邉聡明、橋口陽二郎
  • Organizer: 第１１３回日本外科学会総会
  • Place of Presentation: 福岡
• [Presentation] 大腸癌におけるmicroRNA-21、microRNA-155の臨床的意義とPDCDmRNA、TP53INP1mRNAとの関連性 (2012)
  • Author(s): 飯沼久恵、田村純子、渋谷肇、他
  • Organizer: 第１１２回日本外科学会総会
  • Place of Presentation: 千葉市
• [Presentation] 胃癌におけるmicroRNA-107の臨床的意義およびDICER1mRNAとの関連性 (2012)
  • Author(s): 井上泰介、飯沼久恵、田村純子、他
  • Organizer: 第１１２回日本外科学会総会
  • Place of Presentation: 千葉市
• [Presentation] 大腸癌Dukes stage BおよびC症例における腫瘍環流血中遊離癌細胞のバイオマーカーとしての有用性 (2012)
  • Author(s): 島田竜、飯沼久恵、田村純子、他
  • Organizer: 第３３回癌免疫外科研究会
  • Place of Presentation: 横浜市
• [Presentation] ＴＲＣＲ法による早期乳癌患者における高精度センチネルリンパ節転移診断 (2012)
  • Author(s): 田村純子、飯沼久恵、尾本大輔、他
  • Organizer: 第３３回癌免疫外科研究会
  • Place of Presentation: 横浜市
• [Presentation] 胃癌原発部組織におけるmicroRNA-107発現とその臨床的意義 (2012)
  • Author(s): 井上泰介、飯沼久恵、田村純子、他
  • Organizer: 第３３回癌免疫外科研究会
  • Place of Presentation: 横浜市
• [Presentation] 大腸癌Dukes stage別による、新規癌抑制遺伝子PDCD4の発現と再発および予後との関連性 (2012)
  • Author(s): 堀内敦、飯沼久恵、田村純子
  • Organizer: 第３３回癌免疫外科研究会
  • Place of Presentation: 横浜市
• [Presentation] Clinical significance of circulating tumor cells detection in cancer patients. (2012)
  • Author(s): Hisae Iinuma.
  • Organizer: 5th World CTC Summit
  • Place of Presentation: Singapole
  • Invited
• [Presentation] Prognostic significance of PDCD4 mRNA and association with microRNA-21 in each Dukes stage of colorectal cancer patients. (2012)
  • Author(s): Hisae Iinuma, Atsushi Horiuchi, Junko Tamura, et al.
  • Organizer: 71th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Sapporo
• [Presentation] 消化器癌におけるCnacer stem like cellを指標とした微量癌細胞検出の臨床的意義 (2012)
  • Author(s): 飯沼久恵、田村純子、島田竜、他
  • Organizer: 第５０回日本癌治療学会学術集会（JSCO賞受賞）
  • Place of Presentation: 横浜
• [Presentation] ヒト食道癌cancer stem-like cellに対する細胞障害性T細胞クローンの認識効果 (2012)
  • Author(s): 飯沼久恵、田村純子、小川越史、他
  • Organizer: 第25回日本バイオセラピイ学会学術集会総会（ワークショップ）
  • Place of Presentation: 倉敷
• [Presentation] 乳癌センチネルリンパ節の定量的遺伝子診断の有用性 (2012)
  • Author(s): 田村純子、飯沼久恵、尾本大輔、他
  • Organizer: 第25回日本バイオセラピイ学会学術集会総会
  • Place of Presentation: 倉敷
• [Presentation] 大腸癌におけるDicer1の発現と再発および予後との関連性 (2012)
  • Author(s): 赤羽根拓弥、飯沼久恵、田村純子、他
  • Organizer: 第25回日本バイオセラピイ学会学術集会総会
  • Place of Presentation: 倉敷
• [Presentation] 大腸癌組織におけるmicrRNA-21の発現と予後との関連性 (2012)
  • Author(s): 福島慶久、飯沼久恵、田村純子、他
  • Organizer: 第25回日本バイオセラピイ学会学術集会総会
  • Place of Presentation: 倉敷
• [Presentation] 大腸癌におけるDICER1mRNA発現の予後予測因子としての有用性
  • Author(s): 赤羽根拓弥、飯沼久恵、田村純子、福島慶久、堀内敦、中村圭介、山田英樹、端山軍、野澤慶次郎、石原聡一郎、松田圭二、橋口陽二郎
  • Organizer: 第３４回癌免疫外科研究会
  • Place of Presentation: 岡山
• [Presentation] 胃癌症例におけるmicroRNA-107発現の臨床的意義
  • Author(s): 田村純子、飯沼久恵、井上泰介、堀川昌宏、小川越史、小出泰平、池田佳史、稲葉毅、福島亮治
  • Organizer: 第３４回癌免疫外科研究会
  • Place of Presentation: 岡山
• [Presentation] 大腸癌予後予測マーカーとしての血中循環癌細胞の新たな展開
  • Author(s): 飯沼久恵、三森功士、横堀武彦、田村純子、野澤慶次郎、石原聡一郎、松田圭二、渡邉聡明、橋口陽二郎、森正樹
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] 切除不能食道癌に対する放射線化学療法併用ペプチドワクチン療法の第I相臨床試験
  • Author(s): 田村純子、飯沼久恵、井上泰介、小川越史、堀川昌宏、池田佳史、稲葉毅、吉田浩二、角田卓也、中村祐輔、冲永功太、福島亮治
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] 大腸癌におけるDICER1mRNA発現の予後予測因子としての有用性
  • Author(s): 飯沼久恵、赤羽根拓弥、田村純子、福島慶久、堀内敦、中村圭介、山田英樹、端山軍、野澤慶次郎、藤井正一、松田圭二、橋口陽二郎
  • Organizer: 第５１回日本癌治療学会学術集会
  • Place of Presentation: 京都
• [Presentation] ヒト食道癌の癌幹細胞に対するmultiple CTLクローンによる治療効果増強
  • Author(s): 飯沼久恵、田村純子、井上泰介、小川越史、堀川昌宏、池田佳史、稲葉毅、冲永功太、福島亮治
  • Organizer: 第２６回日本バイオセラピイ学会学術集会総会
  • Place of Presentation: 盛岡
• [Presentation] 大腸癌組織における新規癌抑制遺伝子PDCD4mRNA発現の臨調帝意義とmicrRNA-21との関連性
  • Author(s): 福島慶久、飯沼久恵、田村純子、赤羽根拓弥、堀内敦、中村圭介、土屋剛史、山田英樹、端山軍、野澤慶次郎、藤井正一、松田圭二、橋口陽二郎
  • Organizer: 第２６回日本バイオセラピイ学会学術集会総会
  • Place of Presentation: 盛岡
• [Presentation] 細胞診陰性胃癌症例における腹腔遊離癌幹様細胞遺伝子検出による腹膜再発予測診断の有用性
  • Author(s): 田村純子、飯沼久恵、小川越史、井上泰介、矢口義久、堀川昌宏、池田佳史、稲葉毅、福島亮治
  • Organizer: 第２６回日本バイオセラピイ学会学術集会総会
  • Place of Presentation: 盛岡
• [Presentation] 大腸癌治癒切除における腹腔洗浄液中遊離癌幹様細部を指標とした腹膜再発予測の遺伝子診断
  • Author(s): 端山軍、飯沼久恵、田村純子、福島慶久、赤羽根拓弥、堀内敦、中村圭介、土屋剛史、山田英樹、野澤慶次郎、藤井正一、松田圭二、橋口陽二郎
  • Organizer: 第２６回日本バイオセラピイ学会学術集会総会
  • Place of Presentation: 盛岡