Establishment of hepatocytes differentiation from human multipotent stem cells and investigation for the effectiveness of cell transplantation
| Project/Area Number |
24591999
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
YASUCHIKA Kentaro 京都大学, 医学(系)研究科(研究院), 講師 (00378895)
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| Co-Investigator(Kenkyū-buntansha) |
UEMOTO Shinji 京都大学, 医学(系)研究科(研究院), 教授 (40252449)
ISHII Takamichi 京都大学, 医学(系)研究科(研究院), 助教 (70456789)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 再生医学 / 間葉系細胞 / 細胞移植 / 間葉系幹細胞 / 多能性幹細胞 |
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| Outline of Final Research Achievements |
Human hepatocytes which derived from human MSCs in vitro as previously reported were transplanted into X-SCID rat via portal vein. The transplanted human hepatocytes were incorporated into rat liver and produce albumin and CYP3A4, which represented functonal hepatocytes. In addition, several transplanted hepatocytes showed PCNA activity, which represented the cell progression. Furthermore, the DPPIV expression assay revealed the histological contiguity of transplanted human hepatocytes with rat liver tissue, which representrd the physiological incorporation of transplanted cells. On the otherhand, hepatocytes transplantation into mesentery revealed the effectiveness of hepatocyte transplantation for the rescue of acute lethal liver damage occured by 90% hepatectomy. Transplanted hepatocytes work not only as compensation for hepatic function but also protection of remnant liver tissue, which identified by histological findings and ATP status in remnant liver.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Comparative study of transplantation of hepatocytes at various differentiation stages into mice with lethal liver damage2012
Author(s)
Ryo Kamimura, Takamichi Ishii, Naoya Sasaki, Masatoshi Kajiwara, Takafumi Machimoto, Miwa Saito, Kohno K, Hirofumi Suemori, Norio Nakatsuji, Iwao Ikai, Kentaro Yasuchik, Shinji Uemoto
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Journal Title
Cell Transplant
Volume: 21(11)
Issue: 11
Pages: 2351-62
DOI
Related Report
Peer Reviewed
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[Presentation] THE NEW POSSIBILITY OF AUTOLOGOUS HEPATOCYTE TRANSPLANTATION TO PREVENT ACUTE LIVER FAILURE AFTER MASSIVE HEPATECTOMY2014
Author(s)
S. Kita, K. Yasuchika, T. Ishii, H. Katayama, T. Kawai, S. Ogiso, E.Y. Yoshitoshi, K. Yasuda, M. Kajiwara, K. Fukumitsu, T. Ochiya, E. Hatano, T. Kaido, S. Uemoto
Organizer
49th Annual meeting of the European Association for the Study of the Liver
Place of Presentation
Ｌｏｎｄｏｎ
Year and Date
2014-04-09 – 2014-04-13
Related Report
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