| Project/Area Number |
24592008
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kumamoto University |
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Principal Investigator |
BEPPU toru 熊本大学, 医学部附属病院, 特任教授 (70301372)
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| Co-Investigator(Kenkyū-buntansha) |
OKABE Hirohisa 熊本大学, 医学部附属病院, 診療助手 (40573621)
ANDO Yukio 熊本大学, 大学院生命科学研究部, 教授 (20253742)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 血中癌細胞 / 上皮間葉形質転換 (EMT) / 肝細胞癌 / CD44 / Anoikis / CD45-CD44+CD90+細胞 / 血中癌幹細胞 / 血中癌細胞（CTC） / CD44＋CD90＋細胞 / 上皮間葉形質転換 |
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| Outline of Final Research Achievements |
As the characteristics of hepatocellular carcinoma (HCC), CD44 is correlated with mesenchymal characteristics of HCC and is present downstream of TGF-β signals. After hepatic resection, CD44-positive HCC patients showed poor prognosis (Mima K, Beppu T, et al. Cancer Res 2012). Furthermore we demonstrated that sphere forming ability was very high in CD44 + cells compared to CD44- cells. These characteristics are regulated by Twist1 controlling the mesenchymal characteristics and we confirmed the co-localization of the expression of CD44, Vimentin, Twist1 in human HCC primary tumors (Okabe H, Beppu T, et al. Br J Cancer 2014). As compared with the CD45-CD44 + CD90 + HCC cells in the tissue, the expression of the CD44, and Vimentin in the CD45-CD44 + CD90 + HCC cells in the blood rose markedly, and expression of E-cadherin was dramatically declined. Finally it was confirmed that CD44 is useful as EMT marker for HCC (Okabe H, Beppu T, et al. Br J Cancer 2014).
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