Characterization of circulating tumor cells and its application of the treatment selection for hepatocellular carcinoma
Project/Area Number |
24592008
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Digestive surgery
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Research Institution | Kumamoto University |
Principal Investigator |
BEPPU toru 熊本大学, 医学部附属病院, 特任教授 (70301372)
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Co-Investigator(Kenkyū-buntansha) |
OKABE Hirohisa 熊本大学, 医学部附属病院, 診療助手 (40573621)
ANDO Yukio 熊本大学, 大学院生命科学研究部, 教授 (20253742)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 血中癌細胞 / 上皮間葉形質転換 (EMT) / 肝細胞癌 / CD44 / Anoikis / CD45-CD44+CD90+細胞 / 血中癌幹細胞 / 血中癌細胞(CTC) / CD44+CD90+細胞 / 上皮間葉形質転換 |
Outline of Final Research Achievements |
As the characteristics of hepatocellular carcinoma (HCC), CD44 is correlated with mesenchymal characteristics of HCC and is present downstream of TGF-β signals. After hepatic resection, CD44-positive HCC patients showed poor prognosis (Mima K, Beppu T, et al. Cancer Res 2012). Furthermore we demonstrated that sphere forming ability was very high in CD44 + cells compared to CD44- cells. These characteristics are regulated by Twist1 controlling the mesenchymal characteristics and we confirmed the co-localization of the expression of CD44, Vimentin, Twist1 in human HCC primary tumors (Okabe H, Beppu T, et al. Br J Cancer 2014). As compared with the CD45-CD44 + CD90 + HCC cells in the tissue, the expression of the CD44, and Vimentin in the CD45-CD44 + CD90 + HCC cells in the blood rose markedly, and expression of E-cadherin was dramatically declined. Finally it was confirmed that CD44 is useful as EMT marker for HCC (Okabe H, Beppu T, et al. Br J Cancer 2014).
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] Role of leukocyte cell-derived chemotaxin 2 as a biomarker in hepatocellular carcinoma2014
Author(s)
Okabe H, Delgado E, Lee JM, Yang J, Kinoshita H, Hayashi H, Tsung A, Behari J, Beppu T, Baba H, Monga SP
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Journal Title
PLOS ONE
Volume: 9(6)
Issue: 6
Pages: e98817-e98817
DOI
Related Report
Peer Reviewed
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[Journal Article] CD44s signals the acquisition of the mesenchymal phenotype required for anchorage-independent cell survival in hepatocellular carcinoma.2014
Author(s)
Okabe H, Ishimoto T, Mima K, Nakagawa S, Hayashi H, Kuroki H, Imai K, Nitta H, Saito S, Hashimoto D, Chikamoto A, Ishiko T, Watanabe M, Nagano O, Beppu T, Saya H and Baba H
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Journal Title
Br J Cancer
Volume: 印刷中
Issue: 4
Pages: 958-66
DOI
Related Report
Peer Reviewed
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[Journal Article] CYLD down-regulation might berelated to tumor development in HCCpatients.2013
Author(s)
Kinoshita H, Okabe H, Beppu T,Chikamoto A, Hayashi H, Imai K, MimaK, Nakagawa S, Yokoyama N, Ishiko T,Shinriki S, Jono H, Ando Y, Baba H
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Journal Title
Mol Clin Oncol 1
Volume: 1
Issue: 2
Pages: 309-314
DOI
Related Report
Peer Reviewed
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[Journal Article] Enhancer of Zeste Homolog 2 (EZH2) Promotes Progression of Cholangiocarcinoma Cells by Regulating Cell Cycle and Apoptosis.2013
Author(s)
Nakagawa S, Okabe H, Sakamoto Y, Hayashi H, Hashimoto D, Yokoyama N, Sakamoto K, Kuroki H, Mima K, Nitta H, Imai K, Chikamoto A, Watanabe M, Beppu T, Baba H.
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Journal Title
Ann Surg Oncol
Volume: 3
Issue: S3
Pages: 667-675
DOI
Related Report
Peer Reviewed
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[Journal Article] High CD44s expression is associatedwith the EMT expression profile andintrahepatic dissemination ofhepatocellular carcinoma after localablation therapy.2013
Author(s)
Mima K, Hayashi H,Imai K, Kuroki H,Shigaki N, Nakagawa S, Okabe H,Chikamoto A, Watanabe M, Beppu T, BabaH
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Journal Title
J Hepatobiliary Pancreatic Surg
Volume: 20
Issue: 4
Pages: 429-34
DOI
Related Report
Peer Reviewed
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[Journal Article] Kinoshita H, Okabe H, Beppu T,Chikamoto A, Hayashi H, Imai K, MimaK, Nakagawa S, Ishimoto T, Miyake K,Yokoyama N, Ishiko T, Baba2012
Author(s)
Kinoshita H, Okabe H, Beppu T,Chikamoto A, Hayashi H, Imai K, MimaK, Nakagawa S, Ishimoto T, Miyake K,Yokoyama N, Ishiko T, Baba
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Journal Title
Oncol Rep
Volume: 29
Issue: 2
Pages: 685-9
DOI
Related Report
Peer Reviewed
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