Search of molecules involved in vessel invasion of liver cancer and development of control methods.

• Project/Area Number: 24592015
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Hyogo Medical University
• Principal Investigator: HIRANO TADAMICHI 兵庫医科大学, 医学部, 准教授 (90340968)
• Co-Investigator(Kenkyū-buntansha): IIMURO YUUJI 山梨大学, 総合研究部, 医学研究員 (30252018) ; HAI SEIKAN 兵庫医科大学, 医学部, 講師 (30423833) ; OHASHI KOICHIRO 兵庫医科大学, 医学部, 病院助手 (50573987) ; FUJIMOTO JIRO 兵庫医科大学, 医学部, 教授 (90199373) ; SUEOKA HIDEAKI 兵庫医科大学, 医学部, 助教 (20419831) ; NAKAMURA IKUO 兵庫医科大学, 医学部, 助教 (10625312) ; 裴 正寛 兵庫医科大学, 医学部, 講師 (30423883)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 肝癌 / HCC / 脈管侵襲 / ケモカイン / ICC / CXCR2 / CXCケモカイン / 肝がん

Outline of Final Research Achievements

In the present study, we investigated the relation of chemokine for the vascular invasion of primary liver tumor. First, expression of CXCR2 is estimated by immunohistochemical staining in hepatocellular carcinoma (HCC) with vascular invasion using human specimens.
Expression of several chemokine was measured using ELISA in vitro. Accumulation of myeloid derived suppressor cells (MDSC) was examined immunohistochemically in human samples of HCC. As a result, CXCR2 expression was observed in tumor thrombi than original tumor. CXCL1, 5, 8 were markedly expressed in HCC cell line in vitro. Accumulation of MDSC was confirmed in tumor with vascular invasion compared with tumor without vascular invasion. Our results suggested that chemokine network have a crucial role in the vascular invasion oh HCC.

Research Products

• [Journal Article] Blockage of CXCR2 suppresses tumor growth of intrahepatic cholangiocellular carcinoma. (2014)
  • Author(s): Sueoka H, Hirano T, Uda Y, Iimuro Y, Yamanaka J, Fujimoto J.
  • Journal Title: Surgery Volume: 155 Issue: 4 Pages: 640-649
  • DOI: 10.1016/j.surg.2013.12.037
  • Peer Reviewed
• [Presentation] 肝内胆管癌におけるCXCケモカイン制御による進展抑制の基礎的検討および臨床応用への展望 (2014)
  • Author(s): 平野公通, 末岡英明, 飯室勇二, 岡田敏弘, 近藤祐一, 中村育夫, 田中肖吾, 裵正寛, 山中潤一, 藤元治朗.
  • Organizer: 第69回日本消化器外科学会総会
  • Place of Presentation: 郡山
  • Year and Date: 2014-07-16 – 2014-07-18
• [Presentation] Blockage of CXCR2 suppresses tumor growth of intrahepatic cholangiocellular carcinoma. (2013)
  • Author(s): Hirano T, Sueoka H, Uda Y, Kuroda N, Okada T, Asano Y, Kondo Y, Nakamura I, Tanaka S, Hai S, Iimuro Y, Fujimoto J.
  • Organizer: The 64th Annual Meeting of the American Association for the Study of Liver Diseases (AASLD2013)
  • Place of Presentation: Washington, USA