| Project/Area Number |
24592016
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | National Defense Medical College |
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Principal Investigator |
YAMAMOTO Junji 防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究, その他部局等, 教授 (40342654)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Shunichi 防衛医科大学校(防衛医学研究センター), 准教授 (90502906)
KAWAUCHI Satoko 防衛医科大学校(防衛医学研究センター), 講師 (20506505)
HATSUSE Kazuo 防衛医科大学校(その他の部局等), 准教授 (40082284)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 虚血－再灌流 / 肝臓 / 光計測 / 拡散減衰係数 / 近赤外光 / 虚血再灌流 / 吸収係数 / マウスモデル / 肝虚血 / 肝うっ血 |
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| Outline of Final Research Achievements |
To evaluate usefulness of diffuse reflectance spectroscopy for characterization of viability/microstructure of tissues, we evaluated us’(reduced scattering coefficient), ua (absorption coefficient), StO2 (tissue oxygen saturation), and the reduction of heme aa3 in CcO (cytochrome c oxidase) of in vivo liver tissue using a single-reflectance-fiber probe with two source-collector geometries. We performed in vivo recordings of diffuse reflectance spectra for exposed rat liver of the ischemia-reperfusion model. Significant increases in ua(605)/ua(620) during ischemia indicate the reduction of heme aa3, representing a sign of mitochondrial energy failure. The change in us’ at NIR wavelength region during ischemia might show morphological changes in the cellular and subcellular structures induced by the ischemia. The results of the present study indicate the potential application of the current DRS system to evaluating the pathophysiological conditions of in vivo liver tissue.
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