| Project/Area Number |
24592027
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kobe University |
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Principal Investigator |
ASARI SADAKI 神戸大学, 医学部附属病院, 助教 (20580682)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Ippei 近畿大学, 医学部, 准教授 (30379408)
GOTO Tadahiro 神戸大学, 医学部附属病院, 特命助教 (40580684)
FUKUMOTO Takumi 神戸大学, 医学部附属病院, 准教授 (70379402)
KU Yonoson 神戸大学, 医学部附属病院, 教授 (40195615)
新関 亮 神戸大学, 医学(系)研究科(研究院), 助教 (60444593)
外山 博近 神戸大学, 医学(系)研究科(研究院), 助教 (10444598)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 骨髄細胞 / 間葉系幹細胞 / 膵島 / 1型糖尿病 / 細胞移植 / 膵島細胞 / I型糖尿病 / 移植 / なし |
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| Outline of Final Research Achievements |
(1) We demonstrated that mesenchymal stem cells (MSCs) were purified by culturing the sorted C57BL/6-derived bone marrow cells (BMCs) with the PI(-)CD45(-)Ter119(-)Sca-1(+)PDGFR-α(+) markers.
(2) We showed that transplantation of C57BL/6-derived BMCs (2 x 10^7) induced stable mixed hematopoietic chimerism without GVHD in prediabetic NOD mice preconditioned with temsilorimus (5mg/kg on -day 7 and -day 1), cyclophosphamide (100mg/kg on -day 4 to -day 1) and 2Gy total body irradiation (on day 0). This immunosuppression is a novel regimen based on the clinical transplantation. We also revealed that the induced hematopoietic chimerism completely prevented insulitis and onset of autoimmune diabetes.
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