New development in the study of tumor-stromal interaction focused on extracellular miRNA via exosome.

• Project/Area Number: 24592032
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kyushu University
• Principal Investigator: TOMA Hiroki 九州大学, 医学(系)研究科(研究院), 共同研究員 (80437780)
• Co-Investigator(Kenkyū-buntansha): TANAKA Masao 九州大学, 医学研究院, 教授 (30163570) ; TOMINAGA Yohei 九州大学, 医学研究院, 共同研究員 (90304823) ; OHUCHIDA Kenoki 九州大学, 大学病院, 助教 (20452708) ; MIYASAKA Yoshihiro 九州大学, 医学研究院, 助教 (40507795)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 膵癌 / miRNA / 癌間質相互作用 / microRNA / エクソソーム

Outline of Final Research Achievements

We planed to identify key-miRNAs that correlate with metastasis. First, we checked the effect of miR-373 reported to correlate with metastasis of breast cancer and investigated that transfection of miR-373 induced MET progression in SUIT2 and KP2 cell lines. Next, we analyzed comprehensive miRNA expression of highly metastatic pancreas cancer cell line and normal pancreas cancer cell line. We identified several miRNAs highly expressed in one side. For example, miR-125b-5p, miR-614, miR-3178, miR-4324 and miR-4706 were up-regulated and miR-16-5p, miR-27b-3p, miR-192-5p and miR-194-5p were down-regulated in metastatic cell line. These miRNAs may affect cancer metastasis, because MiR-16, miR-27b, miR-192 and miR-125b were already reported to correlate with metastasis.

Research Products

• [Journal Article] Micro RNA-373 is Down-regulated in Pancreatic Cancer and Inhibits Cancer Cell Invasion (2014)
  • Author(s): Nakata K, Ohuchida K, Mizumoto K, Aishima S, Oda Y, Nagai E, Tanaka M
  • Journal Title: Ann Surg Oncol Volume: 21 Issue: S4 Pages: 564-574
  • DOI: 10.1245/s10434-014-3676-8
  • Peer Reviewed
• [Journal Article] Peritoneal myofibroblasts at metastatic foci promote dissemination of pancreatic cancer. (2014)
  • Author(s): Akagawa S, Ohuchida K, Torata N, Hattori M, Eguchi D, Fujiwara K, Kozono S, Cui L, Ikenaga N, Ohtsuka T, Oishima S, Mizumoto K, Oda Y, Tanaka M
  • Journal Title: Int J Oncol Volume: 45 Issue: 1 Pages: 113-120
  • DOI: 10.3892/ijo.2014.2391
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] CD166/ALCAM expression is characteristic of tumorigenicity and invasive and migratory activities of pancreatic cancer cells. (2014)
  • Author(s): Fujiwara K, Ohuchida K, Sada M, Horioka K, Ulrich CD 3rd, Shindo K, Ohtsuka T, Takahata S, Mizumoto K, Oda Y, Tanaka M.
  • Journal Title: PLoS One Volume: 9 Issue: 9 Pages: 1-11
  • DOI: 10.1371/journal.pone.0107247
  • Peer Reviewed / Open Access
• [Journal Article] MicroRNA-10a is Overexpressed in Human Pancreatic Cancer and Involved in Its Invasiveness Partially via Suppression of the HOXA1 Gene (2012)
  • Author(s): Ohuchida K, Mizumoto K, Lin C, Yamaguchi H, Ohtsuka T, Sato N, Toma H, Nakamura M, Nagai E, Hashizume M, Tanaka M.
  • Journal Title: Ann Surg Oncol Volume: 19(7) Issue: 7 Pages: 2394-2402
  • DOI: 10.1245/s10434-012-2252-3
  • Peer Reviewed
• [Presentation] 膵癌の肝転移におけるfollistatin-like1の役割 (2014)
  • Author(s): 堀岡宏平、大内田研宙、江口大樹、佐田政史、田中友晴、赤川進、藤原謙次、鄭彪、仲田興平、宮坂義浩、前山良、大塚隆生、水元一博、田中雅夫
  • Organizer: 第114回日本外科学会定期学術集会
  • Place of Presentation: 京都市
  • Year and Date: 2014-04-04