Analysis of function of long non-coding RNA associated with liver metastasis from pancreatic cancer

• Project/Area Number: 24592034
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kumamoto University
• Principal Investigator: NAKAHARA osamu 熊本大学, 医学部附属病院, 非常勤診療医師 (40583042)
• Co-Investigator(Kenkyū-buntansha): CHIKAMOTO Akira 熊本大学, 医学部附属病院, 講師 (10419640) ; IMAI Katsunori 熊本大学, 医学部附属病院, 非常勤診療医師 (60555746) ; HAYASHI Hiromitsu 熊本大学, 大学院生命科学研究部, 助教 (80625773) ; NITTA Hidetoshi 熊本大学, 医学部附属病院, 助教 (90555749)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 膵癌 / 転移性肝腫瘍 / HOTAIR / 転移性肝癌 / HOTAIR導入 / 膵臓癌 / Long non-coding RNA

Outline of Final Research Achievements

1.Samples of primary lesions and liver metastasis from same patients.Pancreatic samples were retrieved from patients with unresectable pancreatic cancer at Kumamoto University Hospital.Core-needle biopsies by endoscopic ultrasound-guided fine needle aspiration(EUS-FNA)from the center of the pancreas were immediately snap-frozen in liquid nitrogen and kept at -80℃.Samples of histologically-confirmed pancreatic ductal adenocarcinoma were extracted.Total RNA was prepared by RNA kit(miRVana;、Ambion).Samples have been collected prospectively and the database has been made.We will also retrieve the RNA sample from liver metastasis of the same patients and analyze the expression of HOTAIR.Total RNA samples were obtained from 8 pancreatic cancer cell lines,and real-time RT-PCR was performed.Primers of HOTAIR have been made and then cDNA of them were constructed and analyzed.

Research Products

• [Journal Article] Functional assessment versus conventional volumetric assessment in the prediction of operative outcomes after major hepatectomy (2015)
  • Author(s): Hayashi H, Beppu T, Okabe H, Kuroki H, Nakagawa S, Imai K, Nitta H, Chikamoto A, Ishiko T, Baba H
  • Journal Title: Surgery Volume: 157(1) Issue: 1 Pages: 20-6
  • DOI: 10.1016/j.surg.2014.06.013
  • Peer Reviewed
• [Journal Article] Tumor Shrinkage in Response to Vitamin K2 in Hepatocellular Carcinoma with Multiple Lung Metastases: A Case Report (2014)
  • Author(s): Hayashi H, Higashi T, Takeyama H, Sakamoto K, Kuroki H, Nitta H, Hashimoto D, Chikamoto A, Beppu T, Baba H
  • Journal Title: AJCC Rep Volume: 2(2) Pages: 548-554
  • Peer Reviewed
• [Journal Article] EZH2 Is Associated with Malignant Behavior in Pancreatic IPMN via p27Kip1 Downregulation (2014)
  • Author(s): Kuroki H, Hayashi H, Okabe H, Hashimoto D, Takamori H, Nakahara O, Nakagawa S, Fukushima Y, Chikamoto A, Beppu T, Hirota M, Iyama K, Baba H
  • Journal Title: PLOS ONE Volume: 9(8) Issue: 8 Pages: e100904-e100904
  • DOI: 10.1371/journal.pone.0100904
  • Peer Reviewed
• [Journal Article] Management of thrombocytopenia due to liver cirrhosis; a review (2014)
  • Author(s): Hayashi H, Beppu T, Shirabe K, Maehara Y, Baba H.
  • Journal Title: World J Gastroenterology Volume: 20（10） Issue: 10 Pages: 2595-2605
  • DOI: 10.3748/wjg.v20.i10.2595
  • Peer Reviewed
• [Journal Article] Carcinogenesis of intraductal papillary mucinous neoplasm of the pancreas : loss of microrna-101 promotes overexpression of histone methyltransferase EZH2 (2012)
  • Author(s): Nakahara O
  • Journal Title: Annals of Surgical Oncology Volume: (印刷中) Issue: S3 Pages: 565-71
  • DOI: 10.1245/s10434-011-2068-6
  • Peer Reviewed
• [Presentation] Clinical significance of residual proliferative activity in conversion therapy for colorectal liver-confined metastasis (2014)
  • Author(s): Hayashi H
  • Organizer: SISSO2014
  • Place of Presentation: Grand Hilton Seoul Hotel,Seoul,Korea
• [Presentation] 肝内・肝外胆管癌においてEZH2はアポトーシスを制御し癌の進展に関与する (2012)
  • Author(s): 中川茂樹
  • Organizer: 第67回日本消化器外科学会
  • Place of Presentation: 富山国際会議場
• [Presentation] EZH2 controls cell proliferation of cholangiocarcinoma via the inhibitation of apoptosis. (2012)
  • Author(s): 中川茂樹
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌市教育文化会館
• [Presentation] 膵癌の病態解明と新規バイオマーカーとしてのmiR-101の意義 (2012)
  • Author(s): 中原 修
  • Organizer: 2012年度日本消化器関連学会週間（JDDW 2012）
  • Place of Presentation: 神戸国際展示場
• [Presentation] EZH2は肝外胆管癌において細胞周期・腫瘍増殖を制御して癌の進展に関与する (2012)
  • Author(s): 中川茂樹
  • Organizer: 第23回日本消化器癌発生学会
  • Place of Presentation: 徳島 ルネッサンスリゾートナルト
• [Presentation] 膵IPMNの悪性化におけるEZH2の臨床的意義 (2012)
  • Author(s): 黒木秀幸
  • Organizer: 第23回日本消化器癌発生学会
  • Place of Presentation: 徳島 ルネッサンスリゾートナルト