| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
[Aim] The aim of this study is to explore the relationship between metformin and Hh signaling pathway, and to clarify the mechanism by which metformin prevents cancers. [Materials and methods] Expression of Shh was examined at protein and mRNA level with or without metformin by real-time PCR, immunoblotting and immunestaining. Phosphorylation of AMPK was detected by immunoblotting. [Results] Shh expression was decreased at protein and mRNA level by addition of metformin in BxPC3 cells. Phosphorylation of AMPK was increased by metformin and decreased by dorsomorphin. Interestingly, Shh expression was increased by dorsomorphin. This effect was further confirmed by siRNA of AMPK. [Conclusion] Metformin inhibits production of Shh in BxPC3 through phosphorylation of AMPK. Activation of AMPK might be one of the routes to link metformin and suppression of Shh.
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