| Project/Area Number |
24592046
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Tohoku University |
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Principal Investigator |
MOTOYOSHI NAOTAKA 東北大学, 医学(系)研究科(研究院), 非常勤講師 (40375093)
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| Co-Investigator(Renkei-kenkyūsha) |
SAIKI Yoshikatsu 東北大学, 大学院医学系研究科, 教授 (50372298)
KAWAMOTO Shunsuke 東北大学, 大学院医学系研究科, 准教授 (20400244)
ABE Takaaki 東北大学, 大学院医学系研究科, 教授 (80292209)
NAKAYAMA Keiko 東北大学, 医学系研究科, 教授 (60294972)
OKAMURA Masashi 東北大学, 医学系研究科, 特任助手 (60547397)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
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| Keywords | 腎虚血再灌流障害 / 腎保護 / ユビキチン・プロテアソーム系 / PPAR-γ / LXR-α / 心臓大血管外科 / 心臓大血管周術期腎保護 / ユビチキン・プロテアソーム系 / ＰＰＡＲ－γ |
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| Outline of Final Research Achievements |
We have established the porcine renal ischemia-reperfusion injury model clamping bilateral renal arteries. The pigs were allocated in the two groups; control and Bortezomib (proteasome inhibitor) administration group. We assessed the degrees of kidney damage and expression of PPAR-γ and LXR-α (PPAR-γ’s target gene) in the kidney comparing the Bortezomib administration group with control group. Serum Neutrophil gelatinase-associated lipocalin (NGAL) was used as an indicator of acute kidney injury. Histopathological changings were evaluated by the pathological scoring. Qualification and quantification of the expression of PPAR-γ, LXR-α, and downstream regulatory factors(NFκB, AP-1, NFAT and STAT-3) were evaluated by immunohistochemical staining and real-time RT-PCR method.
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