Role of ASC in cardiac allograft rejection
| Project/Area Number |
24592054
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Shinshu University |
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Principal Investigator |
SETO Tatsuichiro 信州大学, 学術研究院医学系(医学部附属病院), 講師 (70362118)
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| Co-Investigator(Kenkyū-buntansha) |
GOMIBUCHI Toshihito 信州大学, 医学部附属病院, 医員 (90597668)
OHASHI Noburo 信州大学, 医学部附属病院, 医員 (80623272)
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| Co-Investigator(Renkei-kenkyūsha) |
TANIGUCHI Shunichiro 信州大学, 学術研究院医学系, 教授 (60117166)
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| Research Collaborator |
KAMIJO Shinobu 信州大学, 医学部, 技能補佐員
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | インフラマソーム / IL-1β / ASC / 心移植 |
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| Outline of Final Research Achievements |
We used a murine heterotopic cardiac transplantation model using ASC knockout mice. There is no difference in graft survival of fully mismatched model compared to control group. However we found that graft survival of miner mismatched model significantly prolonged compared to control group. Donor hearts were harvested for examination on Days 21 and 42. In ASC knockout group, a slight infiltration of inflammatory cells was observed in the graft and less myocardial injury was seen. The intimal hyperplasia of ASC knockout group was significantly reduced compared to control group. The expression of IL-1β, IL-18 and other inflammatory cytokines were analyzed by immunohistochemistry, real-time reverse transcript-polymerase chain reaction and ELISA.
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Report
(4 results)
Research Products
(4 results)
