Creation of transplant heart with decellulalized heart and mesenchymal stem cell

• Project/Area Number: 24592072
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Keio University
• Principal Investigator: KITAHARA HIROTO 慶應義塾大学, 医学部, 助教 (70594729)
• Co-Investigator(Kenkyū-buntansha): OKAMOTO Kazuma 慶應義塾大学, 医学部, 講師 (10338064)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 脱細胞化 / 再細胞化 / 異所性移植 / 移植・再生医療 / 再生医学 / 循環器・高血圧 / 重症心不全 / 細胞移植 / ヒト幹細胞

Outline of Final Research Achievements

At present, the only treatment for severe heart failure is heart transplantation. However, shortage of donor heart has raised a long waiting list and limited its benefit. As an alternative of heart transplantation, regeneration of heart with organ decellularization technique has been applied. Our ultimate goal is to create a whole beating heart fabricated based on an organ scaffold for human heart transplantation.
A porcine heart was harvested and decellularized by perfusion. The whole-heart scaffold was transplanted in a pig by surgical anastomosis using vessel grafts. The transplanted heart scaffold was harvested on day three after transplantation. Histological report showed that blood clot was accumulated in coronary artery. However, blood perfusion was maintained through left to right intra-cardiac shunt.

Research Products

• [Journal Article] Nonvisualized Type III Endoleak Masquerading as Endotension: A Case Report. (2015)
  • Author(s): 3.Yoshitake A, Hachiya T, Itoh T, Kitahara H, Kasai M, Kawaguchi S, Shimizu H.
  • Journal Title: Ann Vasc Surg. Volume: 29(3)
  • Peer Reviewed
• [Journal Article] Management of Right Atrial Hemangioma in a Patient with Antithrombin Deficiency. (2015)
  • Author(s): 2.Kitahara H, Kudo M, Okamoto M, Inaba Y, Sasaki A, Shimizu H.
  • Journal Title: Ann Thorac Surg. Volume: 99(3)
  • Peer Reviewed
• [Journal Article] A Case of In-Site Reconstruction with a Rifampicin-Bonded Gelatin-Sealed Woven Dacron Graft for Prosthetic Graft Infection with Pseudoaneurysms after Ascending Aortic Replacement for Type A Dissection. (2014)
  • Author(s): 4.Iida Y, Ito T, Kitahara H, Takebe M, Nemoto A, Nagumo M, Saito K, Yamaya T, Kanno H, Misumi T.
  • Journal Title: Ann Vasc Dis. Volume: 7(1) Pages: 68-71
  • Peer Reviewed
• [Journal Article] 弁形成術（僧帽弁閉鎖不全） (2014)
  • Author(s): 岡本一真、 四津良平
  • Journal Title: 成人病と生活習慣病 Volume: 44(7) Pages: 842-847
  • Peer Reviewed
• [Journal Article] 人工腱索再建を軸とした僧帽弁形成 (2014)
  • Author(s): 岡本一真、工藤樹彦、四津良平
  • Journal Title: 心臓 Volume: 46(8) Pages: 1050-1053
  • NAID: 130005091757
  • Peer Reviewed
• [Journal Article] 森論文に対するEditorial Comment (2014)
  • Author(s): 岡本一真
  • Journal Title: 心臓 Volume: 46(6) Pages: 797-798
  • NAID: 130005086857
  • Peer Reviewed
• [Journal Article] 用語解説 ステントレス生体弁 Normo (2014)
  • Author(s): 岡本一真
  • Journal Title: 日本循環器学会専門医誌 Volume: 22(2) Pages: 192-192
  • Peer Reviewed
• [Journal Article] 村松論文に対するEditorial Comment (2014)
  • Author(s): 岡本一真
  • Journal Title: 心臓 Volume: 46(10) Pages: 1398-1398
  • NAID: 130005102580
  • Peer Reviewed
• [Journal Article] A case of in-situ reconstruction with a rifampicin-bonded gelatin-sealed woven dacron graft for prosthetic graft infection with pseudoaneurysms after ascending aortic replacement for type a dissection. (2014)
  • Author(s): Iida Y, Ito T, Kitahara H, Takebe M, Nemoto A, Nagumo M, Saito K, Yamaya T,Kanno H, Misumi T.
  • Journal Title: Ann Vasc Dis Volume: 7(1) Pages: 68-71
  • NAID: 130004713587
  • Peer Reviewed
• [Journal Article] Recurrent Mitral Regurgitation Due to Ruptured ePTFE Neochordae after Mitral Valve Repair by the Loop Technique.A Report of Case. (2014)
  • Author(s): Kudo M, Yozu R, Okamoto K.
  • Journal Title: Ann Thorac Cardiovasc Surg Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Multidisciplinary approach to the treatment of cardiac AA amyloidosis and aortic stenosis due to Castleman's disease: A hybrid therapy withtocilizumab and aortic valve replacement. (2014)
  • Author(s): Shirakawa K, Egashira T, Ieda M, Kawaguchi S, Okamoto K, Kudo M, Yokoyama K,Tsuruta H, Murata M, Mikami S, Anzai A, Hayashida K, Kohno T, Maekawa Y, Sano M, Yozu R, Fukuda K.
  • Journal Title: Int J Cardiol. Volume: 173(2）
  • Peer Reviewed
• [Journal Article] Successful detection and surgical treatment of cardiac hemangioma with right ventricular invasion. (2013)
  • Author(s): Iida Y, Ito T, Kitahara H, Takebe M, Nemoto A, Shimokawa R, Yoshitake A,Misumi T.
  • Journal Title: Ann Thorac Surg Volume: 96(4) Pages: 1481-3
  • Peer Reviewed
• [Presentation] 僧帽弁Beating heart evaluation (2014)
  • Author(s): 岡本一真
  • Organizer: 第19回日本Advanced Heart & Vascular Surgery/OPCAB研究会 アカデミック学術集会
  • Place of Presentation: 東京女子医科大学弥生記念講堂（東京都新宿区）
  • Year and Date: 2014-12-11
• [Presentation] MICSの合併症 (2014)
  • Author(s): 岡本一真
  • Organizer: CCT 2014 Surgical
  • Place of Presentation: 神戸国際展示場・ポートピアホテル（兵庫県神戸市）
  • Year and Date: 2014-10-31 – 2014-11-01
• [Presentation] Investigation of predictive value for aortic regurgitation after transcatheter aortic valve implantation (2014)
  • Author(s): Kitahara H, Yoshitake A, Hachiya T, Okamoto K, Kawaguchi S, Inaba Y, Hayashida K, Inohara T, Yashima F, Fukuda K, Shimizu H
  • Organizer: Complex Cardiovascular Therapeutics,
  • Place of Presentation: 神戸国際展示場・ポートピアホテル（兵庫県神戸市）
  • Year and Date: 2014-10-30 – 2014-11-01
• [Presentation] 内視鏡手術技術の心臓血管領域への応用 (2014)
  • Author(s): 岡本一真、工藤樹彦
  • Organizer: 第27回日本内視鏡外科学会
  • Place of Presentation: ホテルメトロポリタン盛岡〈NEWWING/本館〉・アイーナ・マリオス(岩手県盛岡市)
  • Year and Date: 2014-10-02 – 2014-10-04
• [Presentation] Endovascular Treatment For Primary Aortobronchial Fistulas (2014)
  • Author(s): Kitahara H, Yoshitake A, Hachiya T, Kawaguchi S, Haida H, Hirano A, Shimizu H
  • Organizer: The 67th Annual Scientific Meeting of The Japanese Association for Thoracic Surgery
  • Place of Presentation: 福岡国際会議場・福岡サンパレスホテル＆ホール(福岡県博多市)
  • Year and Date: 2014-09-30 – 2014-10-03
• [Presentation] The use of Loop in Loop technique in mitral valve repair (2014)
  • Author(s): Kazuma Okamoto
  • Organizer: 2014 Shanghai Heart Valve Summit
  • Place of Presentation: Radisson Blu Aqua Hotel（AMERICA/CHICAGO)
  • Year and Date: 2014-09-18 – 2014-09-20
• [Presentation] Surgical treatment for annuloaortic ectasia with marfan syndrome (2014)
  • Author(s): Kitahara H, Shimizu H, Yoshitake A, Kawaguchi S, Ito T, Hirano A, Kasai M, Yozu R
  • Organizer: The 42nd Annual Meeting of Japanese Society for Vascular Surgery
  • Place of Presentation: リンクステーションホール青森【青森市文化会館】・ホテル青森（青森県青森市）
  • Year and Date: 2014-05-21 – 2014-05-23
• [Presentation] Comparison between Transcatheter and Minimally Invasive Surgical Closure of Secundum Atrial Septal Defect in Adults (2014)
  • Author(s): Masaki Kodaira, Kazuma Okamoto,Akio Kawamura, Kentaro Hayashida, Shinsuke Yuasa, Yuichiro Maekawa,Rhohei Yozu, Keiichi Fukuda
  • Organizer: Euro PCR 2014
  • Place of Presentation: Palais Des Congrès de Paris（FRANCE/Paris)
  • Year and Date: 2014-05-20 – 2014-05-23
• [Presentation] Surgical Treatment for an Infective Endocarditis with Aortic Annular Abscess (2014)
  • Author(s): Kitahara H, Kudo M, Shimizu H, Okamoto K, Kawaguchi S, Ito T, Hirano A, Kasai M, Yozu R
  • Organizer: 22nd Annual Meeting of the Asian Society for Cardiovascular and Thoracic Surgery22nd Annual Meeting of the Asian Society for Cardiovascular and Thoracic Surgery
  • Place of Presentation: ISTANBUL CONVENTION &EXHIBITION CENTER(ISTANBUL/TURKEY)
  • Year and Date: 2014-04-03 – 2014-04-06
• [Presentation] Setting up a MICS program, MINIMALLY INVASIVE CARDIAC (2014)
  • Author(s): Kazuma Okamoto
  • Organizer: 22nd Annual Meeting of the Asian
  • Place of Presentation: ISTANBUL CONVENTION &EXHIBITION CENTER(ISTANBUL/TURKEY)
  • Year and Date: 2014-04-03 – 2014-04-06
• [Presentation] 小切開僧帽弁手術の現状と将来展望 (2014)
  • Author(s): 岡本一真
  • Organizer: 第102回生涯教育研修セミナー
  • Place of Presentation: 東京會舘（東京）
  • Invited
• [Presentation] 安全な右小開胸僧帽弁手術の確立 (2014)
  • Author(s): 岡本一真、工藤樹彦、川口新治、北原大翔、伊藤隆仁、平野暁教、河西未央、四津良平
  • Organizer: 第44回日本心臓血管外科学会学術総会
  • Place of Presentation: ホテル日航熊本（熊本）
• [Presentation] Minimally Invasive MV Repair in Barlow’s Disease. (2014)
  • Author(s): 岡本一真
  • Organizer: 第44回日本心臓血管外科学会学術集会ランチョンセミナー
  • Place of Presentation: ホテル日航熊本（熊本）
  • Invited
• [Presentation] How would you operate? Young surgeon vs. Experienced surgeon Strategy and Technique. (2014)
  • Author(s): 岡本一真
  • Organizer: 2014 Heart Valve Conference
  • Place of Presentation: グランフロント大阪（大阪）
• [Presentation] VIDEO-ASSISTED CARDIAC SURGERY VIA RIGHT MINI-THORACOTOMY (2013)
  • Author(s): Kazuma Okamoto, Mikihiko Kudo, Hidenobu Takaki, Shinji Kawaguchi, Hiroto Kitahara,Atsushi Nemoto, Shinichi Fukuhara, Ryohei Yozu.
  • Organizer: The 21st Annual Meeting of the Asian Society for Cardiovascular and Thoracic Surgery in 2013
  • Place of Presentation: 神戸インターナショナルカンファレンスセンター（神戸）
• [Presentation] OUTCOME OF AORITC VALVE REPLACEMENT WITH 17-MM SJM REGENT PROSTHETIC VALVE FOR PATIENT WITHA SMALL AORTIC ANNULUS IN ADULT POPULATION. (2013)
  • Author(s): Hiroto Kitahara, Kazuma Okamoto, Mikihiko Kudo, Shinji Kawaguchi, Takahito Ito,Hidenobu Takaki, Hirohumi Haida, Ryohei Yozu.
  • Organizer: The 21st Annual Meeting of the Asian Society for Cardiovascular and Thoracic Surgery in 2013
  • Place of Presentation: 神戸インターナショナルカンファレンスセンター（神戸）
• [Presentation] Non-Resectional Mitral Valve Repair for Barlow's Type Pathology (VIDEO). (2013)
  • Author(s): H.Kitahara, K.Okamoto, M.Kudo, A.Hirano, S.Kawaguchi, H.Takaki, H.Haida, T.Ito,Y.Inaba, R.Yozu.
  • Organizer: AmericanAssociation for Thoracic Surgery, 2013 MITRAL CONCLAVE
  • Place of Presentation: ニューヨーク（アメリカ）
• [Presentation] Endoscopic-Assisted Mini-Thoracotomy Approach As A Routine Approach for Mitral Valve Surgery Society for Heart Valve Disease. (2013)
  • Author(s): Kazuma Okamoto, Mikihiko Kudo, Hiroto Kitahara, Atsushi Nemoto, Hironobu Takaki, Shinji Kawaguchi, Ryohei Yozu.
  • Organizer: 7th Biennial Congress
  • Place of Presentation: ベニス(イタリア）
• [Presentation] 先天性動脈疾患に対するステントグラフト治療の有用性の検討 (2013)
  • Author(s): 北原大翔、志水秀行、吉武明弘、川口新治、伊藤隆仁、平野暁教、河西未央、川口聡、四津良平
  • Organizer: 第54回日本脈管学会総会
  • Place of Presentation: ステーションコンファレンス(東京）
• [Presentation] What do we need to know about anatomy before starting TAVI. (2013)
  • Author(s): Kazuma Okamoto
  • Organizer: CCT2013
  • Place of Presentation: 神戸国際展示場（神戸）
• [Presentation] 内視鏡補助下右小開胸心臓手術の標準手術化～593例の経験から～ ワークショップ 「低侵襲心臓手術の展望」 (2013)
  • Author(s): 岡本一真、高木秀暢、工藤樹彦、四津良平
  • Organizer: 第113回日本外科学会
  • Place of Presentation: 福岡国際会議場（福岡）
• [Presentation] 僧帽弁形成術の成績安定のための工夫とその治療成績 人工腱索再建を中心とした低侵襲僧帽弁形成術 (2013)
  • Author(s): 工藤 樹彦、岡本 一真、山邉 健太郎、高木 秀暢、川口 新治、伊藤 隆仁、灰田 周史、平野暁教、四津良平
  • Organizer: 第113回日本外科学会
  • Place of Presentation: 福岡国際会議場（福岡）
• [Presentation] MICS-MITRAL advantage and pitfalls MICS or MACS? ～提示を中心に。情報の共有を図る～ (2013)
  • Author(s): 岡本一真
  • Organizer: Japan MICS summit in Osaka
  • Place of Presentation: リーガロイヤルホテル大阪（大阪）
• [Presentation] 内視鏡補助下右小開胸心臓手術の現在と未来 シンポジウム「小切開心臓手術（MICS法）の可能性と安全性」 (2013)
  • Author(s): 岡本一真、工藤樹彦、四津良平
  • Organizer: 第61回日本心臓病学会学術集会
  • Place of Presentation: ホテル日航熊本（熊本）
• [Presentation] 金属アレルギー患者に生体弁を用いた大動脈弁置換術を冠動脈バイパス術を施行した１例 (2013)
  • Author(s): 北原大翔、伊藤努、飯田泰功、根本淳、武部元次郎、三角隆彦
  • Organizer: 第１６１回日本胸部外科学会関東甲信越地方会
  • Place of Presentation: 群馬
• [Book] Annual Review 循環器 2013 (2013)
  • Author(s): 岡本一真、四津良平
  • Total Pages: 285
  • Publisher: 中外医学社
• [Book] 日本医事新報 (2013)
  • Author(s): 岡本一真、四津良平
  • Total Pages: 213
  • Publisher: 日本医事新報社
• [Book] ハートナーシング (2013)
  • Author(s): 岡本一真
  • Total Pages: 128
  • Publisher: メディカ出版